Disruption of hmgA by DNA Duplication is Responsible for Hyperpigmentation in a Vibrio anguillarum Strain

Abstract Vibrio anguillarum 531A, isolated from a diseased fish in the Atlantic Ocean, is a mixture composed of about 95 and 5% of highly pigmented cells (strain 531Ad) and cells with normal levels of pigmentation (strain 531Ac), respectively. Analysis of the V. anguillarum 531Ad DNA region encompas...

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Autores principales:	Veronica Batallones, Jennifer Fernandez, Brett Farthing, Jordan Shoemaker, Keizen Li Qian, Kimberly Phan, Eric Fung, Ashley Rivera, Kevin Van, Francesca de la Cruz, Alexandra J. Ferreri, Krystle Burinski, Jackie Zhang, Vicente Lizarraga, Kevin Doan, Kenneth Rocha, German Traglia, Maria S. Ramirez, Marcelo E. Tolmasky
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3335bd8de0424e7a8b235ad9d0c0b6f0
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spelling	oai:doaj.org-article:3335bd8de0424e7a8b235ad9d0c0b6f02021-12-02T15:07:53ZDisruption of hmgA by DNA Duplication is Responsible for Hyperpigmentation in a Vibrio anguillarum Strain10.1038/s41598-019-51126-82045-2322https://doaj.org/article/3335bd8de0424e7a8b235ad9d0c0b6f02019-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-51126-8https://doaj.org/toc/2045-2322Abstract Vibrio anguillarum 531A, isolated from a diseased fish in the Atlantic Ocean, is a mixture composed of about 95 and 5% of highly pigmented cells (strain 531Ad) and cells with normal levels of pigmentation (strain 531Ac), respectively. Analysis of the V. anguillarum 531Ad DNA region encompassing genes involved in the tyrosine metabolism showed a 410-bp duplication within the hmgA gene that results in a frameshift and early termination of translation of the homogentisate 1,2-dioxygenase. We hypothesized that this mutation results in accumulation of homogentisate that is oxidized and polymerized to produce pyomelanin. Introduction in E. coli of recombinant clones carrying the V. anguillarum hppD (4-hydroxyphenylpyruvate-dioxygenase), and a mutated hmgA produced brown colored colonies. Complementation with a recombinant clone harboring hmgA restored the original color to the colonies confirming that in the absence of homogentisate 1,2-dioxygenase the intermediary in tyrosine catabolism homogentisate accumulates and undergoes nonenzymatic oxidation and polymerization resulting in high amounts of the brown pigment. Whole-genome sequence analysis showed that V. anguillarum 531 Ac and 531Ad differ in the hmgA gene mutation and 23 mutations, most of which locate to intergenic regions and insertion sequences.Veronica BatallonesJennifer FernandezBrett FarthingJordan ShoemakerKeizen Li QianKimberly PhanEric FungAshley RiveraKevin VanFrancesca de la CruzAlexandra J. FerreriKrystle BurinskiJackie ZhangVicente LizarragaKevin DoanKenneth RochaGerman TragliaMaria S. RamirezMarcelo E. TolmaskyNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-10 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Veronica Batallones Jennifer Fernandez Brett Farthing Jordan Shoemaker Keizen Li Qian Kimberly Phan Eric Fung Ashley Rivera Kevin Van Francesca de la Cruz Alexandra J. Ferreri Krystle Burinski Jackie Zhang Vicente Lizarraga Kevin Doan Kenneth Rocha German Traglia Maria S. Ramirez Marcelo E. Tolmasky Disruption of hmgA by DNA Duplication is Responsible for Hyperpigmentation in a Vibrio anguillarum Strain
description	Abstract Vibrio anguillarum 531A, isolated from a diseased fish in the Atlantic Ocean, is a mixture composed of about 95 and 5% of highly pigmented cells (strain 531Ad) and cells with normal levels of pigmentation (strain 531Ac), respectively. Analysis of the V. anguillarum 531Ad DNA region encompassing genes involved in the tyrosine metabolism showed a 410-bp duplication within the hmgA gene that results in a frameshift and early termination of translation of the homogentisate 1,2-dioxygenase. We hypothesized that this mutation results in accumulation of homogentisate that is oxidized and polymerized to produce pyomelanin. Introduction in E. coli of recombinant clones carrying the V. anguillarum hppD (4-hydroxyphenylpyruvate-dioxygenase), and a mutated hmgA produced brown colored colonies. Complementation with a recombinant clone harboring hmgA restored the original color to the colonies confirming that in the absence of homogentisate 1,2-dioxygenase the intermediary in tyrosine catabolism homogentisate accumulates and undergoes nonenzymatic oxidation and polymerization resulting in high amounts of the brown pigment. Whole-genome sequence analysis showed that V. anguillarum 531 Ac and 531Ad differ in the hmgA gene mutation and 23 mutations, most of which locate to intergenic regions and insertion sequences.
format	article
author	Veronica Batallones Jennifer Fernandez Brett Farthing Jordan Shoemaker Keizen Li Qian Kimberly Phan Eric Fung Ashley Rivera Kevin Van Francesca de la Cruz Alexandra J. Ferreri Krystle Burinski Jackie Zhang Vicente Lizarraga Kevin Doan Kenneth Rocha German Traglia Maria S. Ramirez Marcelo E. Tolmasky
author_facet	Veronica Batallones Jennifer Fernandez Brett Farthing Jordan Shoemaker Keizen Li Qian Kimberly Phan Eric Fung Ashley Rivera Kevin Van Francesca de la Cruz Alexandra J. Ferreri Krystle Burinski Jackie Zhang Vicente Lizarraga Kevin Doan Kenneth Rocha German Traglia Maria S. Ramirez Marcelo E. Tolmasky
author_sort	Veronica Batallones
title	Disruption of hmgA by DNA Duplication is Responsible for Hyperpigmentation in a Vibrio anguillarum Strain
title_short	Disruption of hmgA by DNA Duplication is Responsible for Hyperpigmentation in a Vibrio anguillarum Strain
title_full	Disruption of hmgA by DNA Duplication is Responsible for Hyperpigmentation in a Vibrio anguillarum Strain
title_fullStr	Disruption of hmgA by DNA Duplication is Responsible for Hyperpigmentation in a Vibrio anguillarum Strain
title_full_unstemmed	Disruption of hmgA by DNA Duplication is Responsible for Hyperpigmentation in a Vibrio anguillarum Strain
title_sort	disruption of hmga by dna duplication is responsible for hyperpigmentation in a vibrio anguillarum strain
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/3335bd8de0424e7a8b235ad9d0c0b6f0
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Disruption of hmgA by DNA Duplication is Responsible for Hyperpigmentation in a Vibrio anguillarum Strain

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