Dynamic Urinary Proteome Changes in Ovalbumin-Induced Asthma Mouse Model Using Data-Independent Acquisition Proteomics

Weiwei Qin,1,2 Ting Wang,2 Guangwei Liu,3 Lixin Sun,1 Wei Han,4 Youhe Gao2 1Department of Anesthesiology, Qingdao Municipal Hospital, Qingdao University, Qingdao, 266071, People’s Republic of China; 2Department of Biochemistry and Molecular Biology, Gene Engineering Drug and Biotechnology Beijing Ke...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Qin W, Wang T, Liu G, Sun L, Han W, Gao Y
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	ova-induced asthma mice urine proteome data-independent acquisition Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/333aedec988a49508ff68ddd72bf615b
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:333aedec988a49508ff68ddd72bf615b
record_format	dspace
spelling	oai:doaj.org-article:333aedec988a49508ff68ddd72bf615b2021-11-09T18:40:35ZDynamic Urinary Proteome Changes in Ovalbumin-Induced Asthma Mouse Model Using Data-Independent Acquisition Proteomics1178-6965https://doaj.org/article/333aedec988a49508ff68ddd72bf615b2021-11-01T00:00:00Zhttps://www.dovepress.com/dynamic-urinary-proteome-changes-in-ovalbumin-induced-asthma-mouse-mod-peer-reviewed-fulltext-article-JAAhttps://doaj.org/toc/1178-6965Weiwei Qin,1,2 Ting Wang,2 Guangwei Liu,3 Lixin Sun,1 Wei Han,4 Youhe Gao2 1Department of Anesthesiology, Qingdao Municipal Hospital, Qingdao University, Qingdao, 266071, People’s Republic of China; 2Department of Biochemistry and Molecular Biology, Gene Engineering Drug and Biotechnology Beijing Key Laboratory, Beijing Normal University, Beijing, 100875, People’s Republic of China; 3Key Laboratory of Cell Proliferation and Regulation Biology, Ministry of Education, Institute of Cell Biology, College of Life Sciences, Beijing Normal University, Beijing, 100875, People’s Republic of China; 4Department of Respiratory Medicine, Qingdao Municipal Hospital, Qingdao University, Qingdao, 266071, People’s Republic of ChinaCorrespondence: Youhe Gao; Wei Han Tel +86 10 58804382; +86 532 85937809Email gaoyouhe@bnu.edu.cn; sallyhan1@163.comBackground: In this work, we aim to investigate dynamic urinary proteome changes during asthma development and to identify potential urinary protein biomarkers for the diagnosis of asthma.Methods: An ovalbumin (OVA)-induced mouse model was used to mimic asthma. The urinary proteome from asthma and control mice was determined using data-independent acquisition combined with high-resolution tandem mass spectrometry.Results: Overall, 331 proteins were identified, among which 53 were differentially expressed (26, 24, 14 and 20 on days 2, 8, 15 and 18, respectively; 1.5-fold change, adjust P< 0.05). Gene Ontology annotation of the differential proteins showed that the acute-phase response, innate immune response, B cell receptor signaling pathway, and complement activation were significantly enriched. Protein–protein interaction network revealed that these differential proteins were partially biologically connected in OVA-induced asthma, as a group. On days 2 and 8, after two episodes of OVA sensitization, six differential proteins (CRAMP, ECP, HP, F2, AGP1, and CFB) were also reported to be closely associated with asthma. These proteins may hold the potential for the early screening of asthma. On days 15 and 18, after challenged with 1% OVA by inhalation, seven differential proteins (VDBP, HP, CTSE, PIGR, AAT, TRFE, and HPX) were also reported to be closely associated with asthma. Thus, these proteins hold the potential to be biomarkers for the diagnosis of asthma attack.Conclusion: Our results indicate that the urinary proteome could reflect dynamic pathophysiological changes in asthma progression.Keywords: OVA-induced asthma, mice, urine, proteome, data-independent acquisitionQin WWang TLiu GSun LHan WGao YDove Medical Pressarticleova-induced asthmamiceurineproteomedata-independent acquisitionImmunologic diseases. AllergyRC581-607ENJournal of Asthma and Allergy, Vol Volume 14, Pp 1355-1366 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	ova-induced asthma mice urine proteome data-independent acquisition Immunologic diseases. Allergy RC581-607
spellingShingle	ova-induced asthma mice urine proteome data-independent acquisition Immunologic diseases. Allergy RC581-607 Qin W Wang T Liu G Sun L Han W Gao Y Dynamic Urinary Proteome Changes in Ovalbumin-Induced Asthma Mouse Model Using Data-Independent Acquisition Proteomics
description	Weiwei Qin,1,2 Ting Wang,2 Guangwei Liu,3 Lixin Sun,1 Wei Han,4 Youhe Gao2 1Department of Anesthesiology, Qingdao Municipal Hospital, Qingdao University, Qingdao, 266071, People’s Republic of China; 2Department of Biochemistry and Molecular Biology, Gene Engineering Drug and Biotechnology Beijing Key Laboratory, Beijing Normal University, Beijing, 100875, People’s Republic of China; 3Key Laboratory of Cell Proliferation and Regulation Biology, Ministry of Education, Institute of Cell Biology, College of Life Sciences, Beijing Normal University, Beijing, 100875, People’s Republic of China; 4Department of Respiratory Medicine, Qingdao Municipal Hospital, Qingdao University, Qingdao, 266071, People’s Republic of ChinaCorrespondence: Youhe Gao; Wei Han Tel +86 10 58804382; +86 532 85937809Email gaoyouhe@bnu.edu.cn; sallyhan1@163.comBackground: In this work, we aim to investigate dynamic urinary proteome changes during asthma development and to identify potential urinary protein biomarkers for the diagnosis of asthma.Methods: An ovalbumin (OVA)-induced mouse model was used to mimic asthma. The urinary proteome from asthma and control mice was determined using data-independent acquisition combined with high-resolution tandem mass spectrometry.Results: Overall, 331 proteins were identified, among which 53 were differentially expressed (26, 24, 14 and 20 on days 2, 8, 15 and 18, respectively; 1.5-fold change, adjust P< 0.05). Gene Ontology annotation of the differential proteins showed that the acute-phase response, innate immune response, B cell receptor signaling pathway, and complement activation were significantly enriched. Protein–protein interaction network revealed that these differential proteins were partially biologically connected in OVA-induced asthma, as a group. On days 2 and 8, after two episodes of OVA sensitization, six differential proteins (CRAMP, ECP, HP, F2, AGP1, and CFB) were also reported to be closely associated with asthma. These proteins may hold the potential for the early screening of asthma. On days 15 and 18, after challenged with 1% OVA by inhalation, seven differential proteins (VDBP, HP, CTSE, PIGR, AAT, TRFE, and HPX) were also reported to be closely associated with asthma. Thus, these proteins hold the potential to be biomarkers for the diagnosis of asthma attack.Conclusion: Our results indicate that the urinary proteome could reflect dynamic pathophysiological changes in asthma progression.Keywords: OVA-induced asthma, mice, urine, proteome, data-independent acquisition
format	article
author	Qin W Wang T Liu G Sun L Han W Gao Y
author_facet	Qin W Wang T Liu G Sun L Han W Gao Y
author_sort	Qin W
title	Dynamic Urinary Proteome Changes in Ovalbumin-Induced Asthma Mouse Model Using Data-Independent Acquisition Proteomics
title_short	Dynamic Urinary Proteome Changes in Ovalbumin-Induced Asthma Mouse Model Using Data-Independent Acquisition Proteomics
title_full	Dynamic Urinary Proteome Changes in Ovalbumin-Induced Asthma Mouse Model Using Data-Independent Acquisition Proteomics
title_fullStr	Dynamic Urinary Proteome Changes in Ovalbumin-Induced Asthma Mouse Model Using Data-Independent Acquisition Proteomics
title_full_unstemmed	Dynamic Urinary Proteome Changes in Ovalbumin-Induced Asthma Mouse Model Using Data-Independent Acquisition Proteomics
title_sort	dynamic urinary proteome changes in ovalbumin-induced asthma mouse model using data-independent acquisition proteomics
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/333aedec988a49508ff68ddd72bf615b
work_keys_str_mv	AT qinw dynamicurinaryproteomechangesinovalbumininducedasthmamousemodelusingdataindependentacquisitionproteomics AT wangt dynamicurinaryproteomechangesinovalbumininducedasthmamousemodelusingdataindependentacquisitionproteomics AT liug dynamicurinaryproteomechangesinovalbumininducedasthmamousemodelusingdataindependentacquisitionproteomics AT sunl dynamicurinaryproteomechangesinovalbumininducedasthmamousemodelusingdataindependentacquisitionproteomics AT hanw dynamicurinaryproteomechangesinovalbumininducedasthmamousemodelusingdataindependentacquisitionproteomics AT gaoy dynamicurinaryproteomechangesinovalbumininducedasthmamousemodelusingdataindependentacquisitionproteomics
_version_	1718440825732464640

Dynamic Urinary Proteome Changes in Ovalbumin-Induced Asthma Mouse Model Using Data-Independent Acquisition Proteomics

Ejemplares similares