Escherichia coli O127 group 4 capsule proteins assemble at the outer membrane.
Enteropathogenic Escherichia coli O127 is encapsulated by a protective layer of polysaccharide made of the same strain specific O-antigen as the serotype lipopolysaccharide. Seven genes encoding capsule export functions comprise the group 4 capsule (gfc) operon. Genes gfcE, etk and etp encode homolo...
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2021
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oai:doaj.org-article:333b0ce93bb94bbeb53fe28f8142ebd62021-12-02T20:13:06ZEscherichia coli O127 group 4 capsule proteins assemble at the outer membrane.1932-620310.1371/journal.pone.0259900https://doaj.org/article/333b0ce93bb94bbeb53fe28f8142ebd62021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0259900https://doaj.org/toc/1932-6203Enteropathogenic Escherichia coli O127 is encapsulated by a protective layer of polysaccharide made of the same strain specific O-antigen as the serotype lipopolysaccharide. Seven genes encoding capsule export functions comprise the group 4 capsule (gfc) operon. Genes gfcE, etk and etp encode homologs of the group 1 capsule secretion system but the upstream gfcABCD genes encode unknown functions specific to group 4 capsule export. We have developed an expression system for the large-scale production of the outer membrane protein GfcD. Contrary to annotations, we find that GfcD is a non-acylated integral membrane protein. Circular dichroism spectroscopy, light-scattering data, and the HHomp server suggested that GfcD is a monomeric β-barrel with 26 β-strands and an internal globular domain. We identified a set of novel protein-protein interactions between GfcB, GfcC, and GfcD, both in vivo and in vitro, and quantified the binding properties with isothermal calorimetry and biolayer interferometry. GfcC and GfcB form a high-affinity heterodimer with a KD near 100 nM. This heterodimer binds to GfcD (KD = 28 μM) significantly better than either GfcB or GfcC alone. These gfc proteins may form a complex at the outer membrane for group 4 capsule secretion or for a yet unknown function.Matthew R LarsonKassia BiddleAdam GormanSarah BoutomIlan RosenshineMark A SaperPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 11, p e0259900 (2021) |
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Medicine R Science Q Matthew R Larson Kassia Biddle Adam Gorman Sarah Boutom Ilan Rosenshine Mark A Saper Escherichia coli O127 group 4 capsule proteins assemble at the outer membrane. |
description |
Enteropathogenic Escherichia coli O127 is encapsulated by a protective layer of polysaccharide made of the same strain specific O-antigen as the serotype lipopolysaccharide. Seven genes encoding capsule export functions comprise the group 4 capsule (gfc) operon. Genes gfcE, etk and etp encode homologs of the group 1 capsule secretion system but the upstream gfcABCD genes encode unknown functions specific to group 4 capsule export. We have developed an expression system for the large-scale production of the outer membrane protein GfcD. Contrary to annotations, we find that GfcD is a non-acylated integral membrane protein. Circular dichroism spectroscopy, light-scattering data, and the HHomp server suggested that GfcD is a monomeric β-barrel with 26 β-strands and an internal globular domain. We identified a set of novel protein-protein interactions between GfcB, GfcC, and GfcD, both in vivo and in vitro, and quantified the binding properties with isothermal calorimetry and biolayer interferometry. GfcC and GfcB form a high-affinity heterodimer with a KD near 100 nM. This heterodimer binds to GfcD (KD = 28 μM) significantly better than either GfcB or GfcC alone. These gfc proteins may form a complex at the outer membrane for group 4 capsule secretion or for a yet unknown function. |
format |
article |
author |
Matthew R Larson Kassia Biddle Adam Gorman Sarah Boutom Ilan Rosenshine Mark A Saper |
author_facet |
Matthew R Larson Kassia Biddle Adam Gorman Sarah Boutom Ilan Rosenshine Mark A Saper |
author_sort |
Matthew R Larson |
title |
Escherichia coli O127 group 4 capsule proteins assemble at the outer membrane. |
title_short |
Escherichia coli O127 group 4 capsule proteins assemble at the outer membrane. |
title_full |
Escherichia coli O127 group 4 capsule proteins assemble at the outer membrane. |
title_fullStr |
Escherichia coli O127 group 4 capsule proteins assemble at the outer membrane. |
title_full_unstemmed |
Escherichia coli O127 group 4 capsule proteins assemble at the outer membrane. |
title_sort |
escherichia coli o127 group 4 capsule proteins assemble at the outer membrane. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2021 |
url |
https://doaj.org/article/333b0ce93bb94bbeb53fe28f8142ebd6 |
work_keys_str_mv |
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1718374814656233472 |