Antibacterial abilities and biocompatibilities of Ti-Ag alloys with nanotubular coatings

Xingwang Liu,1 Ang Tian,2 Junhua You,3 Hangzhou Zhang,4 Lin Wu,5 Xizhuang Bai,1 Zeming Lei,1 Xiaoguo Shi,2 Xiangxin Xue,2 Hanning Wang4 1Department of Orthopedics, The People’s Hospital of China Medical University, 2Liaoning Provincial Universities Key Laboratory of Boron Resource Ecologi...

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Autores principales:	Liu X, Tian A, You J, Zhang H, Wu L, Bai X, Lei Z, Shi X, Xue X, Wang H
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2016
Materias:	titanium silver nanotube antibacterial biocompatibility Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/333d2e2f96c54835bbc9937deee0d0e9
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spelling	oai:doaj.org-article:333d2e2f96c54835bbc9937deee0d0e92021-12-02T05:24:00ZAntibacterial abilities and biocompatibilities of Ti-Ag alloys with nanotubular coatings1178-2013https://doaj.org/article/333d2e2f96c54835bbc9937deee0d0e92016-11-01T00:00:00Zhttps://www.dovepress.com/antibacterial-abilities-and-biocompatibilities-of-ti-ag-alloys-with-na-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Xingwang Liu,1 Ang Tian,2 Junhua You,3 Hangzhou Zhang,4 Lin Wu,5 Xizhuang Bai,1 Zeming Lei,1 Xiaoguo Shi,2 Xiangxin Xue,2 Hanning Wang4 1Department of Orthopedics, The People’s Hospital of China Medical University, 2Liaoning Provincial Universities Key Laboratory of Boron Resource Ecological Utilization Technology and Boron Materials, Northeastern University, 3School of Materials Science and Engineering, Shenyang University of Technology, 4Department of Sports Medicine and Joint Surgery, The First Affiliated Hospital of China Medical University, 5Department of Prosthodontics, School of Stomatology, China Medical University, Shenyang, People’s Republic of China Purpose: To endow implants with both short- and long-term antibacterial activities without impairing their biocompatibility, novel Ti–Ag alloy substrates with different proportions of Ag (1, 2, and 4 wt% Ag) were generated with nanotubular coverings (TiAg-NT). Methods: Unlike commercial pure Ti and titania nanotube, the TiAg-NT samples exhibited short-term antibacterial activity against Staphylococcus aureus (S. aureus), as confirmed by scanning electron microscopy and double staining with SYTO 9 and propidium iodide. A film applicator coating assay and a zone of inhibition assay were performed to investigate the long-term antibacterial activities of the samples. The cellular viability and cytotoxicity were evaluated through a Cell Counting Kit-8 assay. Annexin V-FITC/propidium iodide double staining was used to assess the level of MG63 cell apoptosis on each sample. Results: All of the TiAg-NT samples, particularly the nanotube-coated Ti–Ag alloy with 2 wt% Ag (Ti2%Ag-NT), could effectively inhibit bacterial adhesion and kill the majority of adhered S. aureus on the first day of culture. Additionally, the excellent antibacterial abilities exhibited by the TiAg-NT samples were sustained for at least 30 days. Although Ti2%Ag-NT had less biocompatibility than titania nanotube, its performance was satisfactory, as demonstrated by the higher cellular viability and lower cell apoptosis rate obtained with it compared with those achieved with commercial pure Ti. The Ti1%Ag-NT and Ti4%Ag-NT samples did not yield good cell viability. Conclusion: This study indicates that the TiAg-NT samples can prevent biofilm formation and maintain their antibacterial ability for at least 1 month. Ti2%Ag-NT exhibited better antibacterial ability and biocompatibility than commercial pure Ti, which could be attributed to the synergistic effect of the presence of Ag (2 wt%) and the morphology of the nanotubes. Ti2%Ag-NT may offer a potential implant material that is capable of preventing implant-related infection. Keywords: titanium, silver, nanotube, antibacterial, biocompatibilityLiu XTian AYou JZhang HWu LBai XLei ZShi XXue XWang HDove Medical PressarticletitaniumsilvernanotubeantibacterialbiocompatibilityMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 11, Pp 5743-5755 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	titanium silver nanotube antibacterial biocompatibility Medicine (General) R5-920
spellingShingle	titanium silver nanotube antibacterial biocompatibility Medicine (General) R5-920 Liu X Tian A You J Zhang H Wu L Bai X Lei Z Shi X Xue X Wang H Antibacterial abilities and biocompatibilities of Ti-Ag alloys with nanotubular coatings
description	Xingwang Liu,1 Ang Tian,2 Junhua You,3 Hangzhou Zhang,4 Lin Wu,5 Xizhuang Bai,1 Zeming Lei,1 Xiaoguo Shi,2 Xiangxin Xue,2 Hanning Wang4 1Department of Orthopedics, The People’s Hospital of China Medical University, 2Liaoning Provincial Universities Key Laboratory of Boron Resource Ecological Utilization Technology and Boron Materials, Northeastern University, 3School of Materials Science and Engineering, Shenyang University of Technology, 4Department of Sports Medicine and Joint Surgery, The First Affiliated Hospital of China Medical University, 5Department of Prosthodontics, School of Stomatology, China Medical University, Shenyang, People’s Republic of China Purpose: To endow implants with both short- and long-term antibacterial activities without impairing their biocompatibility, novel Ti–Ag alloy substrates with different proportions of Ag (1, 2, and 4 wt% Ag) were generated with nanotubular coverings (TiAg-NT). Methods: Unlike commercial pure Ti and titania nanotube, the TiAg-NT samples exhibited short-term antibacterial activity against Staphylococcus aureus (S. aureus), as confirmed by scanning electron microscopy and double staining with SYTO 9 and propidium iodide. A film applicator coating assay and a zone of inhibition assay were performed to investigate the long-term antibacterial activities of the samples. The cellular viability and cytotoxicity were evaluated through a Cell Counting Kit-8 assay. Annexin V-FITC/propidium iodide double staining was used to assess the level of MG63 cell apoptosis on each sample. Results: All of the TiAg-NT samples, particularly the nanotube-coated Ti–Ag alloy with 2 wt% Ag (Ti2%Ag-NT), could effectively inhibit bacterial adhesion and kill the majority of adhered S. aureus on the first day of culture. Additionally, the excellent antibacterial abilities exhibited by the TiAg-NT samples were sustained for at least 30 days. Although Ti2%Ag-NT had less biocompatibility than titania nanotube, its performance was satisfactory, as demonstrated by the higher cellular viability and lower cell apoptosis rate obtained with it compared with those achieved with commercial pure Ti. The Ti1%Ag-NT and Ti4%Ag-NT samples did not yield good cell viability. Conclusion: This study indicates that the TiAg-NT samples can prevent biofilm formation and maintain their antibacterial ability for at least 1 month. Ti2%Ag-NT exhibited better antibacterial ability and biocompatibility than commercial pure Ti, which could be attributed to the synergistic effect of the presence of Ag (2 wt%) and the morphology of the nanotubes. Ti2%Ag-NT may offer a potential implant material that is capable of preventing implant-related infection. Keywords: titanium, silver, nanotube, antibacterial, biocompatibility
format	article
author	Liu X Tian A You J Zhang H Wu L Bai X Lei Z Shi X Xue X Wang H
author_facet	Liu X Tian A You J Zhang H Wu L Bai X Lei Z Shi X Xue X Wang H
author_sort	Liu X
title	Antibacterial abilities and biocompatibilities of Ti-Ag alloys with nanotubular coatings
title_short	Antibacterial abilities and biocompatibilities of Ti-Ag alloys with nanotubular coatings
title_full	Antibacterial abilities and biocompatibilities of Ti-Ag alloys with nanotubular coatings
title_fullStr	Antibacterial abilities and biocompatibilities of Ti-Ag alloys with nanotubular coatings
title_full_unstemmed	Antibacterial abilities and biocompatibilities of Ti-Ag alloys with nanotubular coatings
title_sort	antibacterial abilities and biocompatibilities of ti-ag alloys with nanotubular coatings
publisher	Dove Medical Press
publishDate	2016
url	https://doaj.org/article/333d2e2f96c54835bbc9937deee0d0e9
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Antibacterial abilities and biocompatibilities of Ti-Ag alloys with nanotubular coatings

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