Restraint Stress in Hypertensive Rats Activates the Intestinal Macrophages and Reduces Intestinal Barrier Accompanied by Intestinal Flora Dysbiosis

Tiantian Wang,1 Lei Gao,2 Zejun Yang,3 Feifei Wang,3 Yuexin Guo,4 Boya Wang,5 Rongxuan Hua,3 Hongwei Shang,6 Jingdong Xu1 1Department of Physiology and Pathophysiology, Basic Medical College, Capital Medical University, Beijing, 100069, People’s Republic of China; 2Department of Biomedical...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Wang T, Gao L, Yang Z, Wang F, Guo Y, Wang B, Hua R, Shang H, Xu J
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	hypertension restraint stress intestinal flora juvenile rat intestinal barrier. Pathology RB1-214 Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/3343226eada84f3c8bb2905ab9f569ab
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:3343226eada84f3c8bb2905ab9f569ab
record_format	dspace
spelling	oai:doaj.org-article:3343226eada84f3c8bb2905ab9f569ab2021-12-02T17:04:18ZRestraint Stress in Hypertensive Rats Activates the Intestinal Macrophages and Reduces Intestinal Barrier Accompanied by Intestinal Flora Dysbiosis1178-7031https://doaj.org/article/3343226eada84f3c8bb2905ab9f569ab2021-03-01T00:00:00Zhttps://www.dovepress.com/restraint-stress-in-hypertensive-rats-activates-the-intestinal-macroph-peer-reviewed-article-JIRhttps://doaj.org/toc/1178-7031Tiantian Wang,1 Lei Gao,2 Zejun Yang,3 Feifei Wang,3 Yuexin Guo,4 Boya Wang,5 Rongxuan Hua,3 Hongwei Shang,6 Jingdong Xu1 1Department of Physiology and Pathophysiology, Basic Medical College, Capital Medical University, Beijing, 100069, People’s Republic of China; 2Department of Biomedical Informatics, School of Biomedical Engineering, Capital Medical University, Beijing, 100069, People’s Republic of China; 3Department of Clinical Medicine, Basic Medical College, Capital Medical University, Beijing, 100069, People’s Republic of China; 4Department of Oral Medicine, Basic Medical College, Capital Medical University, Beijing, 100069, People’s Republic of China; 5Eight Program of Clinical Medicine, Peking University Health Science Center, Beijing, 100081, People’s Republic of China; 6Experimental Center for Morphological Research Platform, Capital Medical University, Beijing, 100069, People’s Republic of ChinaCorrespondence: Jingdong Xu Tel +86-10-83911469Email xujingdong@ccmu.edu.cnPurpose: Hypertension (HTN) is a major risk factor for cardiovascular disease. In recent years, there were numerous studies on the function of stress in HTN. However, the gut dysbiosis linked to hypertension in animal models under stress is still incompletely understood. Purpose of this study is to use multiple determination method to determine the juvenile stage intestinal bacteria, cytokines and changes in hormone levels.Methods: Four groups of juvenile male spontaneously hypertensive rats (SHRs) and age-matched male Wistar-Kyoto (WKY) rats were randomly selected as control and experimental groups. Rats in the two stress groups were exposed to restraint stress for 3 hours per day for 7 consecutive days. In one day three times in the method of non-invasive type tail-cuff monitoring blood pressure. The detailed mechanism was illuminated based on the intestinal change using immunohistochemical and immunofluorescence staining and the stress-related hormone and inflammation factors were analyzed via ELISA method. The integrity of the epithelial barrier was assessed using FITC/HRP and the expression levels of proteins associated with the tight junction was detected by Western blot. The alteration of stress-related intestinal flora from ileocecal junction and distal colon were also analyzed using its 16S rDNA sequencing.Results: The results indicate that acute stress rapidly increases mean arterial pressure which is positive correlation to hormone concentration, especially in SHR-stress group. Meanwhile, stress promoted the enhancement of epithelial permeability accompanied with a reduced expression of the tight junction-related protein and the macrophages (M&phis;) aggregation to the lamina propria. There were remarkable significant increase of stress-related hormones and pro-inflammatory factor interleukin (IL)-6 along with a decrease in the diversity of intestinal flora and an imbalance in the F/B ratio.Conclusion: Our results reveal that stress accompanied with HTN could significantly disrupt the domino effect between intestinal flora and homeostasis.Keywords: hypertension, restraint stress, intestinal flora, juvenile rat, intestinal barrier, macrophagesWang TGao LYang ZWang FGuo YWang BHua RShang HXu JDove Medical Pressarticlehypertensionrestraint stressintestinal florajuvenile ratintestinal barrier.PathologyRB1-214Therapeutics. PharmacologyRM1-950ENJournal of Inflammation Research, Vol Volume 14, Pp 1085-1110 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	hypertension restraint stress intestinal flora juvenile rat intestinal barrier. Pathology RB1-214 Therapeutics. Pharmacology RM1-950
spellingShingle	hypertension restraint stress intestinal flora juvenile rat intestinal barrier. Pathology RB1-214 Therapeutics. Pharmacology RM1-950 Wang T Gao L Yang Z Wang F Guo Y Wang B Hua R Shang H Xu J Restraint Stress in Hypertensive Rats Activates the Intestinal Macrophages and Reduces Intestinal Barrier Accompanied by Intestinal Flora Dysbiosis
description	Tiantian Wang,1 Lei Gao,2 Zejun Yang,3 Feifei Wang,3 Yuexin Guo,4 Boya Wang,5 Rongxuan Hua,3 Hongwei Shang,6 Jingdong Xu1 1Department of Physiology and Pathophysiology, Basic Medical College, Capital Medical University, Beijing, 100069, People’s Republic of China; 2Department of Biomedical Informatics, School of Biomedical Engineering, Capital Medical University, Beijing, 100069, People’s Republic of China; 3Department of Clinical Medicine, Basic Medical College, Capital Medical University, Beijing, 100069, People’s Republic of China; 4Department of Oral Medicine, Basic Medical College, Capital Medical University, Beijing, 100069, People’s Republic of China; 5Eight Program of Clinical Medicine, Peking University Health Science Center, Beijing, 100081, People’s Republic of China; 6Experimental Center for Morphological Research Platform, Capital Medical University, Beijing, 100069, People’s Republic of ChinaCorrespondence: Jingdong Xu Tel +86-10-83911469Email xujingdong@ccmu.edu.cnPurpose: Hypertension (HTN) is a major risk factor for cardiovascular disease. In recent years, there were numerous studies on the function of stress in HTN. However, the gut dysbiosis linked to hypertension in animal models under stress is still incompletely understood. Purpose of this study is to use multiple determination method to determine the juvenile stage intestinal bacteria, cytokines and changes in hormone levels.Methods: Four groups of juvenile male spontaneously hypertensive rats (SHRs) and age-matched male Wistar-Kyoto (WKY) rats were randomly selected as control and experimental groups. Rats in the two stress groups were exposed to restraint stress for 3 hours per day for 7 consecutive days. In one day three times in the method of non-invasive type tail-cuff monitoring blood pressure. The detailed mechanism was illuminated based on the intestinal change using immunohistochemical and immunofluorescence staining and the stress-related hormone and inflammation factors were analyzed via ELISA method. The integrity of the epithelial barrier was assessed using FITC/HRP and the expression levels of proteins associated with the tight junction was detected by Western blot. The alteration of stress-related intestinal flora from ileocecal junction and distal colon were also analyzed using its 16S rDNA sequencing.Results: The results indicate that acute stress rapidly increases mean arterial pressure which is positive correlation to hormone concentration, especially in SHR-stress group. Meanwhile, stress promoted the enhancement of epithelial permeability accompanied with a reduced expression of the tight junction-related protein and the macrophages (M&phis;) aggregation to the lamina propria. There were remarkable significant increase of stress-related hormones and pro-inflammatory factor interleukin (IL)-6 along with a decrease in the diversity of intestinal flora and an imbalance in the F/B ratio.Conclusion: Our results reveal that stress accompanied with HTN could significantly disrupt the domino effect between intestinal flora and homeostasis.Keywords: hypertension, restraint stress, intestinal flora, juvenile rat, intestinal barrier, macrophages
format	article
author	Wang T Gao L Yang Z Wang F Guo Y Wang B Hua R Shang H Xu J
author_facet	Wang T Gao L Yang Z Wang F Guo Y Wang B Hua R Shang H Xu J
author_sort	Wang T
title	Restraint Stress in Hypertensive Rats Activates the Intestinal Macrophages and Reduces Intestinal Barrier Accompanied by Intestinal Flora Dysbiosis
title_short	Restraint Stress in Hypertensive Rats Activates the Intestinal Macrophages and Reduces Intestinal Barrier Accompanied by Intestinal Flora Dysbiosis
title_full	Restraint Stress in Hypertensive Rats Activates the Intestinal Macrophages and Reduces Intestinal Barrier Accompanied by Intestinal Flora Dysbiosis
title_fullStr	Restraint Stress in Hypertensive Rats Activates the Intestinal Macrophages and Reduces Intestinal Barrier Accompanied by Intestinal Flora Dysbiosis
title_full_unstemmed	Restraint Stress in Hypertensive Rats Activates the Intestinal Macrophages and Reduces Intestinal Barrier Accompanied by Intestinal Flora Dysbiosis
title_sort	restraint stress in hypertensive rats activates the intestinal macrophages and reduces intestinal barrier accompanied by intestinal flora dysbiosis
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/3343226eada84f3c8bb2905ab9f569ab
work_keys_str_mv	AT wangt restraintstressinhypertensiveratsactivatestheintestinalmacrophagesandreducesintestinalbarrieraccompaniedbyintestinalfloradysbiosis AT gaol restraintstressinhypertensiveratsactivatestheintestinalmacrophagesandreducesintestinalbarrieraccompaniedbyintestinalfloradysbiosis AT yangz restraintstressinhypertensiveratsactivatestheintestinalmacrophagesandreducesintestinalbarrieraccompaniedbyintestinalfloradysbiosis AT wangf restraintstressinhypertensiveratsactivatestheintestinalmacrophagesandreducesintestinalbarrieraccompaniedbyintestinalfloradysbiosis AT guoy restraintstressinhypertensiveratsactivatestheintestinalmacrophagesandreducesintestinalbarrieraccompaniedbyintestinalfloradysbiosis AT wangb restraintstressinhypertensiveratsactivatestheintestinalmacrophagesandreducesintestinalbarrieraccompaniedbyintestinalfloradysbiosis AT huar restraintstressinhypertensiveratsactivatestheintestinalmacrophagesandreducesintestinalbarrieraccompaniedbyintestinalfloradysbiosis AT shangh restraintstressinhypertensiveratsactivatestheintestinalmacrophagesandreducesintestinalbarrieraccompaniedbyintestinalfloradysbiosis AT xuj restraintstressinhypertensiveratsactivatestheintestinalmacrophagesandreducesintestinalbarrieraccompaniedbyintestinalfloradysbiosis
_version_	1718381839306981376

Restraint Stress in Hypertensive Rats Activates the Intestinal Macrophages and Reduces Intestinal Barrier Accompanied by Intestinal Flora Dysbiosis

Ejemplares similares