Homeostatic plasticity and burst activity are mediated by hyperpolarization-activated cation currents and T-type calcium channels in neuronal cultures

Abstract Homeostatic plasticity stabilizes neuronal networks by adjusting the responsiveness of neurons according to their global activity and the intensity of the synaptic inputs. We investigated the homeostatic regulation of hyperpolarization-activated cyclic nucleotide-gated (HCN) and T-type calc...

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Autores principales:	Anikó Rátkai, Krisztián Tárnok, Hajar El Aouad, Brigitta Micska, Katalin Schlett, Attila Szücs
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/334952c2bf854dcea3da2c611ef90029
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spelling	oai:doaj.org-article:334952c2bf854dcea3da2c611ef900292021-12-02T10:44:08ZHomeostatic plasticity and burst activity are mediated by hyperpolarization-activated cation currents and T-type calcium channels in neuronal cultures10.1038/s41598-021-82775-32045-2322https://doaj.org/article/334952c2bf854dcea3da2c611ef900292021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-82775-3https://doaj.org/toc/2045-2322Abstract Homeostatic plasticity stabilizes neuronal networks by adjusting the responsiveness of neurons according to their global activity and the intensity of the synaptic inputs. We investigated the homeostatic regulation of hyperpolarization-activated cyclic nucleotide-gated (HCN) and T-type calcium (CaV3) channels in dissociated and organotypic slice cultures. After 48 h blocking of neuronal activity by tetrodotoxin (TTX), our patch-clamp experiments revealed an increase in the depolarizing voltage sag and post-inhibitory rebound mediated by HCN and CaV3 channels, respectively. All HCN subunits (HCN1 to 4) and T-type Ca-channel subunits (CaV3.1, 3.2 and 3.3) were expressed in both control and activity-deprived hippocampal cultures. Elevated expression levels of CaV3.1 mRNA and a selective increase in the expression of TRIP8b exon 4 isoforms, known to regulate HCN channel localization, were also detected in TTX-treated cultured hippocampal neurons. Immunohistochemical staining in TTX-treated organotypic slices verified a more proximal translocation of HCN1 channels in CA1 pyramidal neurons. Computational modeling also implied that HCN and T-type calcium channels have important role in the regulation of synchronized bursting evoked by previous activity-deprivation. Thus, our findings indicate that HCN and T-type Ca-channels contribute to the homeostatic regulation of excitability and integrative properties of hippocampal neurons.Anikó RátkaiKrisztián TárnokHajar El AouadBrigitta MicskaKatalin SchlettAttila SzücsNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-17 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Anikó Rátkai Krisztián Tárnok Hajar El Aouad Brigitta Micska Katalin Schlett Attila Szücs Homeostatic plasticity and burst activity are mediated by hyperpolarization-activated cation currents and T-type calcium channels in neuronal cultures
description	Abstract Homeostatic plasticity stabilizes neuronal networks by adjusting the responsiveness of neurons according to their global activity and the intensity of the synaptic inputs. We investigated the homeostatic regulation of hyperpolarization-activated cyclic nucleotide-gated (HCN) and T-type calcium (CaV3) channels in dissociated and organotypic slice cultures. After 48 h blocking of neuronal activity by tetrodotoxin (TTX), our patch-clamp experiments revealed an increase in the depolarizing voltage sag and post-inhibitory rebound mediated by HCN and CaV3 channels, respectively. All HCN subunits (HCN1 to 4) and T-type Ca-channel subunits (CaV3.1, 3.2 and 3.3) were expressed in both control and activity-deprived hippocampal cultures. Elevated expression levels of CaV3.1 mRNA and a selective increase in the expression of TRIP8b exon 4 isoforms, known to regulate HCN channel localization, were also detected in TTX-treated cultured hippocampal neurons. Immunohistochemical staining in TTX-treated organotypic slices verified a more proximal translocation of HCN1 channels in CA1 pyramidal neurons. Computational modeling also implied that HCN and T-type calcium channels have important role in the regulation of synchronized bursting evoked by previous activity-deprivation. Thus, our findings indicate that HCN and T-type Ca-channels contribute to the homeostatic regulation of excitability and integrative properties of hippocampal neurons.
format	article
author	Anikó Rátkai Krisztián Tárnok Hajar El Aouad Brigitta Micska Katalin Schlett Attila Szücs
author_facet	Anikó Rátkai Krisztián Tárnok Hajar El Aouad Brigitta Micska Katalin Schlett Attila Szücs
author_sort	Anikó Rátkai
title	Homeostatic plasticity and burst activity are mediated by hyperpolarization-activated cation currents and T-type calcium channels in neuronal cultures
title_short	Homeostatic plasticity and burst activity are mediated by hyperpolarization-activated cation currents and T-type calcium channels in neuronal cultures
title_full	Homeostatic plasticity and burst activity are mediated by hyperpolarization-activated cation currents and T-type calcium channels in neuronal cultures
title_fullStr	Homeostatic plasticity and burst activity are mediated by hyperpolarization-activated cation currents and T-type calcium channels in neuronal cultures
title_full_unstemmed	Homeostatic plasticity and burst activity are mediated by hyperpolarization-activated cation currents and T-type calcium channels in neuronal cultures
title_sort	homeostatic plasticity and burst activity are mediated by hyperpolarization-activated cation currents and t-type calcium channels in neuronal cultures
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/334952c2bf854dcea3da2c611ef90029
work_keys_str_mv	AT anikoratkai homeostaticplasticityandburstactivityaremediatedbyhyperpolarizationactivatedcationcurrentsandttypecalciumchannelsinneuronalcultures AT krisztiantarnok homeostaticplasticityandburstactivityaremediatedbyhyperpolarizationactivatedcationcurrentsandttypecalciumchannelsinneuronalcultures AT hajarelaouad homeostaticplasticityandburstactivityaremediatedbyhyperpolarizationactivatedcationcurrentsandttypecalciumchannelsinneuronalcultures AT brigittamicska homeostaticplasticityandburstactivityaremediatedbyhyperpolarizationactivatedcationcurrentsandttypecalciumchannelsinneuronalcultures AT katalinschlett homeostaticplasticityandburstactivityaremediatedbyhyperpolarizationactivatedcationcurrentsandttypecalciumchannelsinneuronalcultures AT attilaszucs homeostaticplasticityandburstactivityaremediatedbyhyperpolarizationactivatedcationcurrentsandttypecalciumchannelsinneuronalcultures
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Homeostatic plasticity and burst activity are mediated by hyperpolarization-activated cation currents and T-type calcium channels in neuronal cultures

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