Femoral µCT Analysis, Mechanical Testing and Immunolocalization of Bone Proteins in β-Hydroxy β-Methylbutyrate (HMB) Supplemented Spiny Mouse in a Model of Pregnancy and Lactation-Associated Osteoporosis
A metabolite of leucine, ß-hydroxy-ß-methylbutyrate (HMB), used as a dietary supplement effects muscle tissue gain and bone tissue quality. Since there are no studies on the effects of HMB during pregnancy yet, the aim of the current study was to determine the effects of HMB supplementation during p...
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MDPI AG
2021
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oai:doaj.org-article:334a62f6925f4012a98d9a3e0e6daf502021-11-11T17:29:06ZFemoral µCT Analysis, Mechanical Testing and Immunolocalization of Bone Proteins in β-Hydroxy β-Methylbutyrate (HMB) Supplemented Spiny Mouse in a Model of Pregnancy and Lactation-Associated Osteoporosis10.3390/jcm102148082077-0383https://doaj.org/article/334a62f6925f4012a98d9a3e0e6daf502021-10-01T00:00:00Zhttps://www.mdpi.com/2077-0383/10/21/4808https://doaj.org/toc/2077-0383A metabolite of leucine, ß-hydroxy-ß-methylbutyrate (HMB), used as a dietary supplement effects muscle tissue gain and bone tissue quality. Since there are no studies on the effects of HMB during pregnancy yet, the aim of the current study was to determine the effects of HMB supplementation during pregnancy on osteoporotic bone quality postpartum and post-lactation using spiny mice (<i>Acomys cahirinus</i>) as the animal models. The six-month-old dams were divided into four groups: pregnant and lactating controls, and pregnant and lactating HMB-treated (during the second trimester of pregnancy) females. The intensity of the immunoreaction of osteocalcin (OC), osteoprotegerin (OPG), bone morphogenetic protein 2 (BMP-2), tissue inhibitor of metalloproteinases 2 (TIMP-2), matrix metalloproteinase 8 and 13 (MMP-8 and MMP-13) and proteins involved in bone turnover, was measured in femoral trabecular and compact bone, as well as in the hyaline and epiphyseal cartilage of the femora. The analysis of the trabecular bone microarchitecture showed that the administration of HMB to pregnant females, by influencing the proteins responsible for bone cell activity and collagen remodeling, can provide protection from bone loss. Based on the results of the current study it can be assumed that HMB administration to pregnant females has a more positive impact on trabecular than compact bone.Ewa TomaszewskaSiemowit MuszyńskiJanine DonaldsonPiotr DobrowolskiDeepesh K. P. ChandAgnieszka Tomczyk-WarunekMonika Hułas-StasiakIwona PuzioKrzysztof LamorskiCezary SławińskiMirosław JabłońskiTomasz BlicharskiMDPI AGarticleβ-hydroxy β-methylbutyratepregnancylactationbonebone qualitybone proteinsMedicineRENJournal of Clinical Medicine, Vol 10, Iss 4808, p 4808 (2021) |
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EN |
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β-hydroxy β-methylbutyrate pregnancy lactation bone bone quality bone proteins Medicine R |
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β-hydroxy β-methylbutyrate pregnancy lactation bone bone quality bone proteins Medicine R Ewa Tomaszewska Siemowit Muszyński Janine Donaldson Piotr Dobrowolski Deepesh K. P. Chand Agnieszka Tomczyk-Warunek Monika Hułas-Stasiak Iwona Puzio Krzysztof Lamorski Cezary Sławiński Mirosław Jabłoński Tomasz Blicharski Femoral µCT Analysis, Mechanical Testing and Immunolocalization of Bone Proteins in β-Hydroxy β-Methylbutyrate (HMB) Supplemented Spiny Mouse in a Model of Pregnancy and Lactation-Associated Osteoporosis |
| description |
A metabolite of leucine, ß-hydroxy-ß-methylbutyrate (HMB), used as a dietary supplement effects muscle tissue gain and bone tissue quality. Since there are no studies on the effects of HMB during pregnancy yet, the aim of the current study was to determine the effects of HMB supplementation during pregnancy on osteoporotic bone quality postpartum and post-lactation using spiny mice (<i>Acomys cahirinus</i>) as the animal models. The six-month-old dams were divided into four groups: pregnant and lactating controls, and pregnant and lactating HMB-treated (during the second trimester of pregnancy) females. The intensity of the immunoreaction of osteocalcin (OC), osteoprotegerin (OPG), bone morphogenetic protein 2 (BMP-2), tissue inhibitor of metalloproteinases 2 (TIMP-2), matrix metalloproteinase 8 and 13 (MMP-8 and MMP-13) and proteins involved in bone turnover, was measured in femoral trabecular and compact bone, as well as in the hyaline and epiphyseal cartilage of the femora. The analysis of the trabecular bone microarchitecture showed that the administration of HMB to pregnant females, by influencing the proteins responsible for bone cell activity and collagen remodeling, can provide protection from bone loss. Based on the results of the current study it can be assumed that HMB administration to pregnant females has a more positive impact on trabecular than compact bone. |
| format |
article |
| author |
Ewa Tomaszewska Siemowit Muszyński Janine Donaldson Piotr Dobrowolski Deepesh K. P. Chand Agnieszka Tomczyk-Warunek Monika Hułas-Stasiak Iwona Puzio Krzysztof Lamorski Cezary Sławiński Mirosław Jabłoński Tomasz Blicharski |
| author_facet |
Ewa Tomaszewska Siemowit Muszyński Janine Donaldson Piotr Dobrowolski Deepesh K. P. Chand Agnieszka Tomczyk-Warunek Monika Hułas-Stasiak Iwona Puzio Krzysztof Lamorski Cezary Sławiński Mirosław Jabłoński Tomasz Blicharski |
| author_sort |
Ewa Tomaszewska |
| title |
Femoral µCT Analysis, Mechanical Testing and Immunolocalization of Bone Proteins in β-Hydroxy β-Methylbutyrate (HMB) Supplemented Spiny Mouse in a Model of Pregnancy and Lactation-Associated Osteoporosis |
| title_short |
Femoral µCT Analysis, Mechanical Testing and Immunolocalization of Bone Proteins in β-Hydroxy β-Methylbutyrate (HMB) Supplemented Spiny Mouse in a Model of Pregnancy and Lactation-Associated Osteoporosis |
| title_full |
Femoral µCT Analysis, Mechanical Testing and Immunolocalization of Bone Proteins in β-Hydroxy β-Methylbutyrate (HMB) Supplemented Spiny Mouse in a Model of Pregnancy and Lactation-Associated Osteoporosis |
| title_fullStr |
Femoral µCT Analysis, Mechanical Testing and Immunolocalization of Bone Proteins in β-Hydroxy β-Methylbutyrate (HMB) Supplemented Spiny Mouse in a Model of Pregnancy and Lactation-Associated Osteoporosis |
| title_full_unstemmed |
Femoral µCT Analysis, Mechanical Testing and Immunolocalization of Bone Proteins in β-Hydroxy β-Methylbutyrate (HMB) Supplemented Spiny Mouse in a Model of Pregnancy and Lactation-Associated Osteoporosis |
| title_sort |
femoral µct analysis, mechanical testing and immunolocalization of bone proteins in β-hydroxy β-methylbutyrate (hmb) supplemented spiny mouse in a model of pregnancy and lactation-associated osteoporosis |
| publisher |
MDPI AG |
| publishDate |
2021 |
| url |
https://doaj.org/article/334a62f6925f4012a98d9a3e0e6daf50 |
| work_keys_str_mv |
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