Synthesis of Nm-PHB (nanomelanin-polyhydroxy butyrate) nanocomposite film and its protective effect against biofilm-forming multi drug resistant Staphylococcus aureus

Abstract Melanin is a dark brown ubiquitous photosynthetic pigment which have many varied and ever expanding applications in fabrication of radio-protective materials, food packaging, cosmetics and in medicine. In this study, melanin production in a Pseudomonas sp. which was isolated from the marine...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	George Seghal Kiran, Stephen A. Jackson, Sethu Priyadharsini, Alan D. W. Dobson, Joseph Selvin
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3350f62ba8f249ffb703fe593c0b760d
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:3350f62ba8f249ffb703fe593c0b760d
record_format	dspace
spelling	oai:doaj.org-article:3350f62ba8f249ffb703fe593c0b760d2021-12-02T15:05:10ZSynthesis of Nm-PHB (nanomelanin-polyhydroxy butyrate) nanocomposite film and its protective effect against biofilm-forming multi drug resistant Staphylococcus aureus10.1038/s41598-017-08816-y2045-2322https://doaj.org/article/3350f62ba8f249ffb703fe593c0b760d2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08816-yhttps://doaj.org/toc/2045-2322Abstract Melanin is a dark brown ubiquitous photosynthetic pigment which have many varied and ever expanding applications in fabrication of radio-protective materials, food packaging, cosmetics and in medicine. In this study, melanin production in a Pseudomonas sp. which was isolated from the marine sponge Tetyrina citirna was optimized employing one-factor at a time experiments and characterized for chemical nature and stability. Following sonication nucleated nanomelanin (Nm) particles were formed and evaluated for antibacterial and antioxidant properties. Nanocomposite film was fabricated using combinations (% w/v) of polyhydroxy butyrate-nanomelanin (PHB:Nm) blended with 1% glycerol. The Nm was found to be spherical in shape with a diameter of 100–140 nm and showed strong antimicrobial activity against both Gram positive and Gram negative bacteria. The Nm-PHB nanocomposite film was homogeneous, smooth, without any cracks, and flexible. XRD and DSC data indicated that the film was crystalline in nature, and was thermostable up to 281.87 °C. This study represents the first report on the synthesis of Nm and fabrication of Nm-PHB nanocomposite film which show strong protective effect against multidrug resistant Staphyloccoccus aureus. Thus this Nm-PHB nanocomposite film may find utility as packaging material for food products by protecting the food products from oxidation and bacterial contamination.George Seghal KiranStephen A. JacksonSethu PriyadharsiniAlan D. W. DobsonJoseph SelvinNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q George Seghal Kiran Stephen A. Jackson Sethu Priyadharsini Alan D. W. Dobson Joseph Selvin Synthesis of Nm-PHB (nanomelanin-polyhydroxy butyrate) nanocomposite film and its protective effect against biofilm-forming multi drug resistant Staphylococcus aureus
description	Abstract Melanin is a dark brown ubiquitous photosynthetic pigment which have many varied and ever expanding applications in fabrication of radio-protective materials, food packaging, cosmetics and in medicine. In this study, melanin production in a Pseudomonas sp. which was isolated from the marine sponge Tetyrina citirna was optimized employing one-factor at a time experiments and characterized for chemical nature and stability. Following sonication nucleated nanomelanin (Nm) particles were formed and evaluated for antibacterial and antioxidant properties. Nanocomposite film was fabricated using combinations (% w/v) of polyhydroxy butyrate-nanomelanin (PHB:Nm) blended with 1% glycerol. The Nm was found to be spherical in shape with a diameter of 100–140 nm and showed strong antimicrobial activity against both Gram positive and Gram negative bacteria. The Nm-PHB nanocomposite film was homogeneous, smooth, without any cracks, and flexible. XRD and DSC data indicated that the film was crystalline in nature, and was thermostable up to 281.87 °C. This study represents the first report on the synthesis of Nm and fabrication of Nm-PHB nanocomposite film which show strong protective effect against multidrug resistant Staphyloccoccus aureus. Thus this Nm-PHB nanocomposite film may find utility as packaging material for food products by protecting the food products from oxidation and bacterial contamination.
format	article
author	George Seghal Kiran Stephen A. Jackson Sethu Priyadharsini Alan D. W. Dobson Joseph Selvin
author_facet	George Seghal Kiran Stephen A. Jackson Sethu Priyadharsini Alan D. W. Dobson Joseph Selvin
author_sort	George Seghal Kiran
title	Synthesis of Nm-PHB (nanomelanin-polyhydroxy butyrate) nanocomposite film and its protective effect against biofilm-forming multi drug resistant Staphylococcus aureus
title_short	Synthesis of Nm-PHB (nanomelanin-polyhydroxy butyrate) nanocomposite film and its protective effect against biofilm-forming multi drug resistant Staphylococcus aureus
title_full	Synthesis of Nm-PHB (nanomelanin-polyhydroxy butyrate) nanocomposite film and its protective effect against biofilm-forming multi drug resistant Staphylococcus aureus
title_fullStr	Synthesis of Nm-PHB (nanomelanin-polyhydroxy butyrate) nanocomposite film and its protective effect against biofilm-forming multi drug resistant Staphylococcus aureus
title_full_unstemmed	Synthesis of Nm-PHB (nanomelanin-polyhydroxy butyrate) nanocomposite film and its protective effect against biofilm-forming multi drug resistant Staphylococcus aureus
title_sort	synthesis of nm-phb (nanomelanin-polyhydroxy butyrate) nanocomposite film and its protective effect against biofilm-forming multi drug resistant staphylococcus aureus
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/3350f62ba8f249ffb703fe593c0b760d
work_keys_str_mv	AT georgeseghalkiran synthesisofnmphbnanomelaninpolyhydroxybutyratenanocompositefilmanditsprotectiveeffectagainstbiofilmformingmultidrugresistantstaphylococcusaureus AT stephenajackson synthesisofnmphbnanomelaninpolyhydroxybutyratenanocompositefilmanditsprotectiveeffectagainstbiofilmformingmultidrugresistantstaphylococcusaureus AT sethupriyadharsini synthesisofnmphbnanomelaninpolyhydroxybutyratenanocompositefilmanditsprotectiveeffectagainstbiofilmformingmultidrugresistantstaphylococcusaureus AT alandwdobson synthesisofnmphbnanomelaninpolyhydroxybutyratenanocompositefilmanditsprotectiveeffectagainstbiofilmformingmultidrugresistantstaphylococcusaureus AT josephselvin synthesisofnmphbnanomelaninpolyhydroxybutyratenanocompositefilmanditsprotectiveeffectagainstbiofilmformingmultidrugresistantstaphylococcusaureus
_version_	1718388883882770432

Synthesis of Nm-PHB (nanomelanin-polyhydroxy butyrate) nanocomposite film and its protective effect against biofilm-forming multi drug resistant Staphylococcus aureus

Ejemplares similares