The Wnt pathway controls cell death engulfment, spindle orientation, and migration through CED-10/Rac.

Wnt signalling pathways have extremely diverse functions in animals, including induction of cell fates or tumours, guidance of cell movements during gastrulation, and the induction of cell polarity. Wnt can induce polar changes in cellular morphology by a remodelling of the cytoskeleton. However, ho...

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Autores principales:	Juan Cabello, Lukas J Neukomm, Ufuk Günesdogan, Katharina Burkart, Steve J Charette, Günter Lochnit, Michael O Hengartner, Ralf Schnabel
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/3361d2f9b06647a58398e9686b03e445
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spelling	oai:doaj.org-article:3361d2f9b06647a58398e9686b03e4452021-11-25T05:34:23ZThe Wnt pathway controls cell death engulfment, spindle orientation, and migration through CED-10/Rac.1544-91731545-788510.1371/journal.pbio.1000297https://doaj.org/article/3361d2f9b06647a58398e9686b03e4452010-02-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20126385/pdf/?tool=EBIhttps://doaj.org/toc/1544-9173https://doaj.org/toc/1545-7885Wnt signalling pathways have extremely diverse functions in animals, including induction of cell fates or tumours, guidance of cell movements during gastrulation, and the induction of cell polarity. Wnt can induce polar changes in cellular morphology by a remodelling of the cytoskeleton. However, how activation of the Frizzled receptor induces cytoskeleton rearrangement is not well understood. We show, by an in depth 4-D microscopy analysis, that the Caenorhabditis elegans Wnt pathway signals to CED-10/Rac via two separate branches to regulate modulation of the cytoskeleton in different cellular situations. Apoptotic cell clearance and migration of the distal tip cell require the MOM-5/Fz receptor, GSK-3 kinase, and APC/APR-1, which activate the CED-2/5/12 branch of the engulfment machinery. MOM-5 (Frizzled) thus can function as an engulfment receptor in C. elegans. Our epistatic analyses also suggest that the two partially redundant signalling pathways defined earlier for engulfment may act in a single pathway in early embryos. By contrast, rearrangement of mitotic spindles requires the MOM-5/Fz receptor, GSK-3 kinase, and beta-catenins, but not the downstream factors LIT-1/NLK or POP-1/Tcf. Taken together, our results indicate that in multiple developmental processes, CED-10/Rac can link polar signals mediated by the Wnt pathway to rearrangements of the cytoskeleton.Juan CabelloLukas J NeukommUfuk GünesdoganKatharina BurkartSteve J CharetteGünter LochnitMichael O HengartnerRalf SchnabelPublic Library of Science (PLoS)articleBiology (General)QH301-705.5ENPLoS Biology, Vol 8, Iss 2, p e1000297 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Juan Cabello Lukas J Neukomm Ufuk Günesdogan Katharina Burkart Steve J Charette Günter Lochnit Michael O Hengartner Ralf Schnabel The Wnt pathway controls cell death engulfment, spindle orientation, and migration through CED-10/Rac.
description	Wnt signalling pathways have extremely diverse functions in animals, including induction of cell fates or tumours, guidance of cell movements during gastrulation, and the induction of cell polarity. Wnt can induce polar changes in cellular morphology by a remodelling of the cytoskeleton. However, how activation of the Frizzled receptor induces cytoskeleton rearrangement is not well understood. We show, by an in depth 4-D microscopy analysis, that the Caenorhabditis elegans Wnt pathway signals to CED-10/Rac via two separate branches to regulate modulation of the cytoskeleton in different cellular situations. Apoptotic cell clearance and migration of the distal tip cell require the MOM-5/Fz receptor, GSK-3 kinase, and APC/APR-1, which activate the CED-2/5/12 branch of the engulfment machinery. MOM-5 (Frizzled) thus can function as an engulfment receptor in C. elegans. Our epistatic analyses also suggest that the two partially redundant signalling pathways defined earlier for engulfment may act in a single pathway in early embryos. By contrast, rearrangement of mitotic spindles requires the MOM-5/Fz receptor, GSK-3 kinase, and beta-catenins, but not the downstream factors LIT-1/NLK or POP-1/Tcf. Taken together, our results indicate that in multiple developmental processes, CED-10/Rac can link polar signals mediated by the Wnt pathway to rearrangements of the cytoskeleton.
format	article
author	Juan Cabello Lukas J Neukomm Ufuk Günesdogan Katharina Burkart Steve J Charette Günter Lochnit Michael O Hengartner Ralf Schnabel
author_facet	Juan Cabello Lukas J Neukomm Ufuk Günesdogan Katharina Burkart Steve J Charette Günter Lochnit Michael O Hengartner Ralf Schnabel
author_sort	Juan Cabello
title	The Wnt pathway controls cell death engulfment, spindle orientation, and migration through CED-10/Rac.
title_short	The Wnt pathway controls cell death engulfment, spindle orientation, and migration through CED-10/Rac.
title_full	The Wnt pathway controls cell death engulfment, spindle orientation, and migration through CED-10/Rac.
title_fullStr	The Wnt pathway controls cell death engulfment, spindle orientation, and migration through CED-10/Rac.
title_full_unstemmed	The Wnt pathway controls cell death engulfment, spindle orientation, and migration through CED-10/Rac.
title_sort	wnt pathway controls cell death engulfment, spindle orientation, and migration through ced-10/rac.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/3361d2f9b06647a58398e9686b03e445
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The Wnt pathway controls cell death engulfment, spindle orientation, and migration through CED-10/Rac.

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