lncRNA myocardial infarction-associated transcript (MIAT) knockdown alleviates LPS-induced chondrocytes inflammatory injury via regulating miR-488-3p/sex determining region Y-related HMG-box 11 (SOX11) axis

Long noncoding RNA (lncRNA) has been shown to be involved in the development of osteoarthritis (OA), an age-related bone and joint disease. However, the function and possible molecular mechanism of lncRNA myocardial infarction-associated transcript (MIAT) in lipopolysaccharide (LPS)-induced chondroc...

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Autores principales:	Pan Weiwei, Wang Haibao, Ruan Jianwei, Zheng Wenbiao, Chen Fanghu, Kong Jinsong, Wang Yong
Formato:	article
Lenguaje:	EN
Publicado:	De Gruyter 2021
Materias:	osteoarthritis lncrna miat mir-488-3p sox11 inflammatory injury Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/337eea4ac2d74ff3baec03f4cd5c3f6c
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spelling	oai:doaj.org-article:337eea4ac2d74ff3baec03f4cd5c3f6c2021-12-05T14:10:40ZlncRNA myocardial infarction-associated transcript (MIAT) knockdown alleviates LPS-induced chondrocytes inflammatory injury via regulating miR-488-3p/sex determining region Y-related HMG-box 11 (SOX11) axis2391-541210.1515/biol-2021-0023https://doaj.org/article/337eea4ac2d74ff3baec03f4cd5c3f6c2021-05-01T00:00:00Zhttps://doi.org/10.1515/biol-2021-0023https://doaj.org/toc/2391-5412Long noncoding RNA (lncRNA) has been shown to be involved in the development of osteoarthritis (OA), an age-related bone and joint disease. However, the function and possible molecular mechanism of lncRNA myocardial infarction-associated transcript (MIAT) in lipopolysaccharide (LPS)-induced chondrocytes injury model remain unexplored. Cell viability and apoptosis were detected by methyl thiazolyl tetrazolium (MTT) and flow cytometry, respectively. Western blot was used to detect protein expression. The concentrations of inflammatory factors were estimated by enzyme-linked immunosorbent assay (ELISA). Abundances of MIAT, microRNA-488-3p (miR-488-3p), and sex determining region Y-related HMG-box 11 (SOX11) were examined by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR). Dual-luciferase reporter and RNA immunoprecipitation (RIP) assays were performed to analyze the interaction between miR-488-3p and MIAT or SOX11. LPS caused chondrocytes injury by reducing cell activity and increasing apoptosis rate and inflammatory factor secretions. Higher levels of MIAT and SOX11 and lower miR-488-3p were observed in LPS-treated C28/I2 cells. Importantly, knockdown of MIAT attenuated the LPS-induced cell injury by targeting miR-488-3p, and miR-488-3p overexpression weakened the LPS-induced cell injury by targeting SOX11. Additionally, repression of MIAT inactivated the LPS-induced NF-κB signaling pathway by decreasing SOX11 and increasing miR-488-3p. Knockdown of MIAT alleviated the LPS-induced chondrocytes injury by inhibiting the NF-κB signaling pathway mediated by the miR-488-3p/SOX11 axis.Pan WeiweiWang HaibaoRuan JianweiZheng WenbiaoChen FanghuKong JinsongWang YongDe Gruyterarticleosteoarthritislncrna miatmir-488-3psox11inflammatory injuryBiology (General)QH301-705.5ENOpen Life Sciences, Vol 16, Iss 1, Pp 511-522 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	osteoarthritis lncrna miat mir-488-3p sox11 inflammatory injury Biology (General) QH301-705.5
spellingShingle	osteoarthritis lncrna miat mir-488-3p sox11 inflammatory injury Biology (General) QH301-705.5 Pan Weiwei Wang Haibao Ruan Jianwei Zheng Wenbiao Chen Fanghu Kong Jinsong Wang Yong lncRNA myocardial infarction-associated transcript (MIAT) knockdown alleviates LPS-induced chondrocytes inflammatory injury via regulating miR-488-3p/sex determining region Y-related HMG-box 11 (SOX11) axis
description	Long noncoding RNA (lncRNA) has been shown to be involved in the development of osteoarthritis (OA), an age-related bone and joint disease. However, the function and possible molecular mechanism of lncRNA myocardial infarction-associated transcript (MIAT) in lipopolysaccharide (LPS)-induced chondrocytes injury model remain unexplored. Cell viability and apoptosis were detected by methyl thiazolyl tetrazolium (MTT) and flow cytometry, respectively. Western blot was used to detect protein expression. The concentrations of inflammatory factors were estimated by enzyme-linked immunosorbent assay (ELISA). Abundances of MIAT, microRNA-488-3p (miR-488-3p), and sex determining region Y-related HMG-box 11 (SOX11) were examined by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR). Dual-luciferase reporter and RNA immunoprecipitation (RIP) assays were performed to analyze the interaction between miR-488-3p and MIAT or SOX11. LPS caused chondrocytes injury by reducing cell activity and increasing apoptosis rate and inflammatory factor secretions. Higher levels of MIAT and SOX11 and lower miR-488-3p were observed in LPS-treated C28/I2 cells. Importantly, knockdown of MIAT attenuated the LPS-induced cell injury by targeting miR-488-3p, and miR-488-3p overexpression weakened the LPS-induced cell injury by targeting SOX11. Additionally, repression of MIAT inactivated the LPS-induced NF-κB signaling pathway by decreasing SOX11 and increasing miR-488-3p. Knockdown of MIAT alleviated the LPS-induced chondrocytes injury by inhibiting the NF-κB signaling pathway mediated by the miR-488-3p/SOX11 axis.
format	article
author	Pan Weiwei Wang Haibao Ruan Jianwei Zheng Wenbiao Chen Fanghu Kong Jinsong Wang Yong
author_facet	Pan Weiwei Wang Haibao Ruan Jianwei Zheng Wenbiao Chen Fanghu Kong Jinsong Wang Yong
author_sort	Pan Weiwei
title	lncRNA myocardial infarction-associated transcript (MIAT) knockdown alleviates LPS-induced chondrocytes inflammatory injury via regulating miR-488-3p/sex determining region Y-related HMG-box 11 (SOX11) axis
title_short	lncRNA myocardial infarction-associated transcript (MIAT) knockdown alleviates LPS-induced chondrocytes inflammatory injury via regulating miR-488-3p/sex determining region Y-related HMG-box 11 (SOX11) axis
title_full	lncRNA myocardial infarction-associated transcript (MIAT) knockdown alleviates LPS-induced chondrocytes inflammatory injury via regulating miR-488-3p/sex determining region Y-related HMG-box 11 (SOX11) axis
title_fullStr	lncRNA myocardial infarction-associated transcript (MIAT) knockdown alleviates LPS-induced chondrocytes inflammatory injury via regulating miR-488-3p/sex determining region Y-related HMG-box 11 (SOX11) axis
title_full_unstemmed	lncRNA myocardial infarction-associated transcript (MIAT) knockdown alleviates LPS-induced chondrocytes inflammatory injury via regulating miR-488-3p/sex determining region Y-related HMG-box 11 (SOX11) axis
title_sort	lncrna myocardial infarction-associated transcript (miat) knockdown alleviates lps-induced chondrocytes inflammatory injury via regulating mir-488-3p/sex determining region y-related hmg-box 11 (sox11) axis
publisher	De Gruyter
publishDate	2021
url	https://doaj.org/article/337eea4ac2d74ff3baec03f4cd5c3f6c
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lncRNA myocardial infarction-associated transcript (MIAT) knockdown alleviates LPS-induced chondrocytes inflammatory injury via regulating miR-488-3p/sex determining region Y-related HMG-box 11 (SOX11) axis

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