The zebrafish ventricle: A hub of cardiac endothelial cells for in vitro cell behavior studies

Abstract Despite our increasing understanding of zebrafish heart development and regeneration, there is limited information about the distribution of endothelial cells (ECs) in the adult zebrafish heart. Here, we investigate and compare the distribution of cardiac ECs (cECs) in adult mouse and zebra...

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Autores principales:	Chinmoy Patra, Zacharias Kontarakis, Harmandeep Kaur, Amey Rayrikar, Debanjan Mukherjee, Didier Y. R. Stainier
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/338a742f29e9472b8cfbf01b1f352f53
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spelling	oai:doaj.org-article:338a742f29e9472b8cfbf01b1f352f532021-12-02T11:41:11ZThe zebrafish ventricle: A hub of cardiac endothelial cells for in vitro cell behavior studies10.1038/s41598-017-02461-12045-2322https://doaj.org/article/338a742f29e9472b8cfbf01b1f352f532017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02461-1https://doaj.org/toc/2045-2322Abstract Despite our increasing understanding of zebrafish heart development and regeneration, there is limited information about the distribution of endothelial cells (ECs) in the adult zebrafish heart. Here, we investigate and compare the distribution of cardiac ECs (cECs) in adult mouse and zebrafish ventricles. Surprisingly, we find that (i) active coronary vessel growth is present in adult zebrafish, (ii) ~37 and ~39% of cells in the zebrafish heart are ECs and cardiomyocytes, respectively, a composition similar to that seen in mouse. However, we find that in zebrafish, ~36% of the ventricular tissue is covered with ECs, i.e., a substantially larger proportion than in mouse. Capitalising on the high abundance of cECs in zebrafish, we established a protocol to isolate them with high purity using fluorescent transgenic lines. Our approach eliminates side-effects due to antibody utilisation. Moreover, the isolated cECs maintained a high proliferation index even after three passages and were amenable to pharmacological treatments to study cEC migration in vitro. Such primary cultures will be a useful tool for supplementary in vitro studies on the accumulating zebrafish mutant lines as well as the screening of small molecule libraries on cardiac specific endothelial cells.Chinmoy PatraZacharias KontarakisHarmandeep KaurAmey RayrikarDebanjan MukherjeeDidier Y. R. StainierNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Chinmoy Patra Zacharias Kontarakis Harmandeep Kaur Amey Rayrikar Debanjan Mukherjee Didier Y. R. Stainier The zebrafish ventricle: A hub of cardiac endothelial cells for in vitro cell behavior studies
description	Abstract Despite our increasing understanding of zebrafish heart development and regeneration, there is limited information about the distribution of endothelial cells (ECs) in the adult zebrafish heart. Here, we investigate and compare the distribution of cardiac ECs (cECs) in adult mouse and zebrafish ventricles. Surprisingly, we find that (i) active coronary vessel growth is present in adult zebrafish, (ii) ~37 and ~39% of cells in the zebrafish heart are ECs and cardiomyocytes, respectively, a composition similar to that seen in mouse. However, we find that in zebrafish, ~36% of the ventricular tissue is covered with ECs, i.e., a substantially larger proportion than in mouse. Capitalising on the high abundance of cECs in zebrafish, we established a protocol to isolate them with high purity using fluorescent transgenic lines. Our approach eliminates side-effects due to antibody utilisation. Moreover, the isolated cECs maintained a high proliferation index even after three passages and were amenable to pharmacological treatments to study cEC migration in vitro. Such primary cultures will be a useful tool for supplementary in vitro studies on the accumulating zebrafish mutant lines as well as the screening of small molecule libraries on cardiac specific endothelial cells.
format	article
author	Chinmoy Patra Zacharias Kontarakis Harmandeep Kaur Amey Rayrikar Debanjan Mukherjee Didier Y. R. Stainier
author_facet	Chinmoy Patra Zacharias Kontarakis Harmandeep Kaur Amey Rayrikar Debanjan Mukherjee Didier Y. R. Stainier
author_sort	Chinmoy Patra
title	The zebrafish ventricle: A hub of cardiac endothelial cells for in vitro cell behavior studies
title_short	The zebrafish ventricle: A hub of cardiac endothelial cells for in vitro cell behavior studies
title_full	The zebrafish ventricle: A hub of cardiac endothelial cells for in vitro cell behavior studies
title_fullStr	The zebrafish ventricle: A hub of cardiac endothelial cells for in vitro cell behavior studies
title_full_unstemmed	The zebrafish ventricle: A hub of cardiac endothelial cells for in vitro cell behavior studies
title_sort	zebrafish ventricle: a hub of cardiac endothelial cells for in vitro cell behavior studies
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/338a742f29e9472b8cfbf01b1f352f53
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The zebrafish ventricle: A hub of cardiac endothelial cells for in vitro cell behavior studies

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