High throughput error corrected Nanopore single cell transcriptome sequencing

Droplet-based high throughput single cell sequencing techniques can often lose information on transcript splicing and heterogenity. Here the authors introduce ScNaUmi-seq, which uses Oxford Nanopore sequencing and barcoding to generate high accuracy full length sequences.

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Auteurs principaux: Kevin Lebrigand, Virginie Magnone, Pascal Barbry, Rainer Waldmann
Format: article
Langue:EN
Publié: Nature Portfolio 2020
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Accès en ligne:https://doaj.org/article/338e1aedfdba41a0ba4cd63207452b26
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Résumé:Droplet-based high throughput single cell sequencing techniques can often lose information on transcript splicing and heterogenity. Here the authors introduce ScNaUmi-seq, which uses Oxford Nanopore sequencing and barcoding to generate high accuracy full length sequences.