High throughput error corrected Nanopore single cell transcriptome sequencing

Droplet-based high throughput single cell sequencing techniques can often lose information on transcript splicing and heterogenity. Here the authors introduce ScNaUmi-seq, which uses Oxford Nanopore sequencing and barcoding to generate high accuracy full length sequences.

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Autores principales:	Kevin Lebrigand, Virginie Magnone, Pascal Barbry, Rainer Waldmann
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/338e1aedfdba41a0ba4cd63207452b26
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spelling	oai:doaj.org-article:338e1aedfdba41a0ba4cd63207452b262021-12-02T16:27:46ZHigh throughput error corrected Nanopore single cell transcriptome sequencing10.1038/s41467-020-17800-62041-1723https://doaj.org/article/338e1aedfdba41a0ba4cd63207452b262020-08-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-17800-6https://doaj.org/toc/2041-1723Droplet-based high throughput single cell sequencing techniques can often lose information on transcript splicing and heterogenity. Here the authors introduce ScNaUmi-seq, which uses Oxford Nanopore sequencing and barcoding to generate high accuracy full length sequences.Kevin LebrigandVirginie MagnonePascal BarbryRainer WaldmannNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-8 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Kevin Lebrigand Virginie Magnone Pascal Barbry Rainer Waldmann High throughput error corrected Nanopore single cell transcriptome sequencing
description	Droplet-based high throughput single cell sequencing techniques can often lose information on transcript splicing and heterogenity. Here the authors introduce ScNaUmi-seq, which uses Oxford Nanopore sequencing and barcoding to generate high accuracy full length sequences.
format	article
author	Kevin Lebrigand Virginie Magnone Pascal Barbry Rainer Waldmann
author_facet	Kevin Lebrigand Virginie Magnone Pascal Barbry Rainer Waldmann
author_sort	Kevin Lebrigand
title	High throughput error corrected Nanopore single cell transcriptome sequencing
title_short	High throughput error corrected Nanopore single cell transcriptome sequencing
title_full	High throughput error corrected Nanopore single cell transcriptome sequencing
title_fullStr	High throughput error corrected Nanopore single cell transcriptome sequencing
title_full_unstemmed	High throughput error corrected Nanopore single cell transcriptome sequencing
title_sort	high throughput error corrected nanopore single cell transcriptome sequencing
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/338e1aedfdba41a0ba4cd63207452b26
work_keys_str_mv	AT kevinlebrigand highthroughputerrorcorrectednanoporesinglecelltranscriptomesequencing AT virginiemagnone highthroughputerrorcorrectednanoporesinglecelltranscriptomesequencing AT pascalbarbry highthroughputerrorcorrectednanoporesinglecelltranscriptomesequencing AT rainerwaldmann highthroughputerrorcorrectednanoporesinglecelltranscriptomesequencing
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High throughput error corrected Nanopore single cell transcriptome sequencing

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