GLYI and D-LDH play key role in methylglyoxal detoxification and abiotic stress tolerance

Abstract Methylglyoxal(MG) is a potent cytotoxin that is produced as a byproduct of various metabolic reactions in the cell. The major enzymes for MG detoxification are Glyoxalase I(GLYI), Glyoxalase II(GLYII) and D-lactate dehydrogenase(D-LDH). These three enzymes work together and convert MG into...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Muskan Jain, Preeti Nagar, Ayush Sharma, Rituraj Batth, Sakshi Aggarwal, Sumita Kumari, Ananda Mustafiz
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3394687f7df74264bb3857df650f43ec
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:3394687f7df74264bb3857df650f43ec
record_format	dspace
spelling	oai:doaj.org-article:3394687f7df74264bb3857df650f43ec2021-12-02T15:05:17ZGLYI and D-LDH play key role in methylglyoxal detoxification and abiotic stress tolerance10.1038/s41598-018-23806-42045-2322https://doaj.org/article/3394687f7df74264bb3857df650f43ec2018-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-23806-4https://doaj.org/toc/2045-2322Abstract Methylglyoxal(MG) is a potent cytotoxin that is produced as a byproduct of various metabolic reactions in the cell. The major enzymes for MG detoxification are Glyoxalase I(GLYI), Glyoxalase II(GLYII) and D-lactate dehydrogenase(D-LDH). These three enzymes work together and convert MG into D-pyruvate, which directly goes to TCA cycle. Here, a comparative study of the ability of MG detoxification of these three enzymes has been done in both E. coli and yeast. Ectopic expression of these three genes from Arabidopsis in E. coli in presence of different abiotic stress revealed the contribution of each of these genes in detoxifying MG. Yeast mutants of MG detoxification enzymes were also grown in different stress conditions to record the effect of each gene. These mutants were also used for complementation assays using the respective MG detoxifying genes from Arabidopsis in presence of various stress conditions. The MG content and the corresponding growth of cells was measured in all the bacterial as well as yeast strains. This study reveals differential contribution of MG detoxification enzymes in mitigating MG levels and alleviating stress in both prokaryotes as well as eukaryotes. GLYI and D-LDH were found to be key enzymes in MG detoxification under various abiotic stresses.Muskan JainPreeti NagarAyush SharmaRituraj BatthSakshi AggarwalSumita KumariAnanda MustafizNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-9 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Muskan Jain Preeti Nagar Ayush Sharma Rituraj Batth Sakshi Aggarwal Sumita Kumari Ananda Mustafiz GLYI and D-LDH play key role in methylglyoxal detoxification and abiotic stress tolerance
description	Abstract Methylglyoxal(MG) is a potent cytotoxin that is produced as a byproduct of various metabolic reactions in the cell. The major enzymes for MG detoxification are Glyoxalase I(GLYI), Glyoxalase II(GLYII) and D-lactate dehydrogenase(D-LDH). These three enzymes work together and convert MG into D-pyruvate, which directly goes to TCA cycle. Here, a comparative study of the ability of MG detoxification of these three enzymes has been done in both E. coli and yeast. Ectopic expression of these three genes from Arabidopsis in E. coli in presence of different abiotic stress revealed the contribution of each of these genes in detoxifying MG. Yeast mutants of MG detoxification enzymes were also grown in different stress conditions to record the effect of each gene. These mutants were also used for complementation assays using the respective MG detoxifying genes from Arabidopsis in presence of various stress conditions. The MG content and the corresponding growth of cells was measured in all the bacterial as well as yeast strains. This study reveals differential contribution of MG detoxification enzymes in mitigating MG levels and alleviating stress in both prokaryotes as well as eukaryotes. GLYI and D-LDH were found to be key enzymes in MG detoxification under various abiotic stresses.
format	article
author	Muskan Jain Preeti Nagar Ayush Sharma Rituraj Batth Sakshi Aggarwal Sumita Kumari Ananda Mustafiz
author_facet	Muskan Jain Preeti Nagar Ayush Sharma Rituraj Batth Sakshi Aggarwal Sumita Kumari Ananda Mustafiz
author_sort	Muskan Jain
title	GLYI and D-LDH play key role in methylglyoxal detoxification and abiotic stress tolerance
title_short	GLYI and D-LDH play key role in methylglyoxal detoxification and abiotic stress tolerance
title_full	GLYI and D-LDH play key role in methylglyoxal detoxification and abiotic stress tolerance
title_fullStr	GLYI and D-LDH play key role in methylglyoxal detoxification and abiotic stress tolerance
title_full_unstemmed	GLYI and D-LDH play key role in methylglyoxal detoxification and abiotic stress tolerance
title_sort	glyi and d-ldh play key role in methylglyoxal detoxification and abiotic stress tolerance
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/3394687f7df74264bb3857df650f43ec
work_keys_str_mv	AT muskanjain glyianddldhplaykeyroleinmethylglyoxaldetoxificationandabioticstresstolerance AT preetinagar glyianddldhplaykeyroleinmethylglyoxaldetoxificationandabioticstresstolerance AT ayushsharma glyianddldhplaykeyroleinmethylglyoxaldetoxificationandabioticstresstolerance AT riturajbatth glyianddldhplaykeyroleinmethylglyoxaldetoxificationandabioticstresstolerance AT sakshiaggarwal glyianddldhplaykeyroleinmethylglyoxaldetoxificationandabioticstresstolerance AT sumitakumari glyianddldhplaykeyroleinmethylglyoxaldetoxificationandabioticstresstolerance AT anandamustafiz glyianddldhplaykeyroleinmethylglyoxaldetoxificationandabioticstresstolerance
_version_	1718388884101922816

GLYI and D-LDH play key role in methylglyoxal detoxification and abiotic stress tolerance

Ejemplares similares