Microfluidic sorting of intrinsically magnetic cells under visual control

Abstract Magnetic cell sorting provides a valuable complementary mechanism to fluorescent techniques, especially if its parameters can be fine-tuned. In addition, there has recently been growing interest in studying naturally occurring magnetic cells and genetic engineering of cells to render them m...

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Autores principales:	Ahne Myklatun, Michele Cappetta, Michael Winklhofer, Vasilis Ntziachristos, Gil G. Westmeyer
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3397d3d133b84384a8f0bd8a1be92177
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spelling	oai:doaj.org-article:3397d3d133b84384a8f0bd8a1be921772021-12-02T11:52:15ZMicrofluidic sorting of intrinsically magnetic cells under visual control10.1038/s41598-017-06946-x2045-2322https://doaj.org/article/3397d3d133b84384a8f0bd8a1be921772017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06946-xhttps://doaj.org/toc/2045-2322Abstract Magnetic cell sorting provides a valuable complementary mechanism to fluorescent techniques, especially if its parameters can be fine-tuned. In addition, there has recently been growing interest in studying naturally occurring magnetic cells and genetic engineering of cells to render them magnetic in order to control molecular processes via magnetic fields. For such approaches, contamination-free magnetic separation is an essential capability. We here present a robust and tunable microfluidic sorting system in which magnetic gradients of up to 1700 T/m can be applied to cells flowing through a sorting channel by reversible magnetization of ferrofluids. Visual control of the sorting process allowed us to optimize sorting efficiencies for a large range of sizes and magnetic moments of cells. Using automated quantification based on imaging of fluorescent markers, we showed that macrophages containing phagocytosed magnetic nanoparticles, with cellular magnetic dipole moments on the order of 10 fAm2, could be sorted with an efficiency of 90 ± 1%. Furthermore, we successfully sorted intrinsically magnetic magnetotactic bacteria with magnetic moments of 0.1 fAm2. In distinction to column-based magnetic sorting devices, microfluidic systems can prevent sample contact with superparamagnetic material. This ensures contamination-free separation of naturally occurring or bioengineered magnetic cells and is essential for downstream characterization of their properties.Ahne MyklatunMichele CappettaMichael WinklhoferVasilis NtziachristosGil G. WestmeyerNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ahne Myklatun Michele Cappetta Michael Winklhofer Vasilis Ntziachristos Gil G. Westmeyer Microfluidic sorting of intrinsically magnetic cells under visual control
description	Abstract Magnetic cell sorting provides a valuable complementary mechanism to fluorescent techniques, especially if its parameters can be fine-tuned. In addition, there has recently been growing interest in studying naturally occurring magnetic cells and genetic engineering of cells to render them magnetic in order to control molecular processes via magnetic fields. For such approaches, contamination-free magnetic separation is an essential capability. We here present a robust and tunable microfluidic sorting system in which magnetic gradients of up to 1700 T/m can be applied to cells flowing through a sorting channel by reversible magnetization of ferrofluids. Visual control of the sorting process allowed us to optimize sorting efficiencies for a large range of sizes and magnetic moments of cells. Using automated quantification based on imaging of fluorescent markers, we showed that macrophages containing phagocytosed magnetic nanoparticles, with cellular magnetic dipole moments on the order of 10 fAm2, could be sorted with an efficiency of 90 ± 1%. Furthermore, we successfully sorted intrinsically magnetic magnetotactic bacteria with magnetic moments of 0.1 fAm2. In distinction to column-based magnetic sorting devices, microfluidic systems can prevent sample contact with superparamagnetic material. This ensures contamination-free separation of naturally occurring or bioengineered magnetic cells and is essential for downstream characterization of their properties.
format	article
author	Ahne Myklatun Michele Cappetta Michael Winklhofer Vasilis Ntziachristos Gil G. Westmeyer
author_facet	Ahne Myklatun Michele Cappetta Michael Winklhofer Vasilis Ntziachristos Gil G. Westmeyer
author_sort	Ahne Myklatun
title	Microfluidic sorting of intrinsically magnetic cells under visual control
title_short	Microfluidic sorting of intrinsically magnetic cells under visual control
title_full	Microfluidic sorting of intrinsically magnetic cells under visual control
title_fullStr	Microfluidic sorting of intrinsically magnetic cells under visual control
title_full_unstemmed	Microfluidic sorting of intrinsically magnetic cells under visual control
title_sort	microfluidic sorting of intrinsically magnetic cells under visual control
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/3397d3d133b84384a8f0bd8a1be92177
work_keys_str_mv	AT ahnemyklatun microfluidicsortingofintrinsicallymagneticcellsundervisualcontrol AT michelecappetta microfluidicsortingofintrinsicallymagneticcellsundervisualcontrol AT michaelwinklhofer microfluidicsortingofintrinsicallymagneticcellsundervisualcontrol AT vasilisntziachristos microfluidicsortingofintrinsicallymagneticcellsundervisualcontrol AT gilgwestmeyer microfluidicsortingofintrinsicallymagneticcellsundervisualcontrol
_version_	1718395095235952640

Microfluidic sorting of intrinsically magnetic cells under visual control

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