MicroRNA-140-3p represses the proliferation, migration, invasion and angiogenesis of lung adenocarcinoma cells via targeting TYMS (Thymidylate Synthetase)

MicroRNA (miR)-140-3p has been proved to repress lung adenocarcinoma (LUAD), and our study aims to further evaluate the mechanism. Bioinformatic analyses were performed. The viability, proliferation, migration, invasion and angiogenesis of transfected LUAD cells were all determined via Cell Counting...

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Autores principales:	Shanzhi Wan, Zhimin Liu, Yang Chen, Zhitao Mai, Mingming Jiang, Qingguo Di, Baohua Sun
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	lung adenocarcinoma microrna-140-3p proliferation metastasis angiogenesis thymidylate synthetase Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/339c2728e55e404388911ee22047dd0e
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spelling	oai:doaj.org-article:339c2728e55e404388911ee22047dd0e2021-11-26T11:19:50ZMicroRNA-140-3p represses the proliferation, migration, invasion and angiogenesis of lung adenocarcinoma cells via targeting TYMS (Thymidylate Synthetase)2165-59792165-598710.1080/21655979.2021.2009422https://doaj.org/article/339c2728e55e404388911ee22047dd0e2021-11-01T00:00:00Zhttp://dx.doi.org/10.1080/21655979.2021.2009422https://doaj.org/toc/2165-5979https://doaj.org/toc/2165-5987MicroRNA (miR)-140-3p has been proved to repress lung adenocarcinoma (LUAD), and our study aims to further evaluate the mechanism. Bioinformatic analyses were performed. The viability, proliferation, migration, invasion and angiogenesis of transfected LUAD cells were all determined via Cell Counting Kit-8, colony formation, Scratch, Transwell, and tube formation assays. The targeting relationship between miR-140-3p and Thymidylate Synthetase (TYMS) was confirmed by dual-luciferase reporter (DLR) assay. Relative expressions of miR-140-3p, TYMS, metastasis- (E-Cadherin, N-Cadherin, Vimentin), angiogenesis- (vascular endothelial growth factor (VEGF)), and apoptosis-related factors (cleaved Caspase-3, B-cell lymphoma-2 (Bcl-2), Bcl-2-associated X protein (Bax)) were quantified by quantitative real-time polymerase chain reaction or Western blot. TYMS was high-expressed yet miR-140-3p was low-expressed in LUAD cells. Upregulation of miR-140-3p inhibited TYMS expression, viability, colony formation, migration, invasion, and tube length within LUAD cells, while downregulation of miR-140-3p did oppositely. Silenced TYMS, the downstream target gene of miR-140-3p, reversed the effects of miR-140-3p downregulation on TYMS expression, cell viability, colony formation, migration, invasion, and tube length as well as the metastasis-, apoptosis- and angiogenesis-related proteins in LUAD cells. Upregulation of miR-140-3p inhibited the proliferation, migration, invasion and angiogenesis of LUAD cells via targeting TYMS.Shanzhi WanZhimin LiuYang ChenZhitao MaiMingming JiangQingguo DiBaohua SunTaylor & Francis Grouparticlelung adenocarcinomamicrorna-140-3pproliferationmetastasisangiogenesisthymidylate synthetaseBiotechnologyTP248.13-248.65ENBioengineered, Vol 0, Iss 0 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	lung adenocarcinoma microrna-140-3p proliferation metastasis angiogenesis thymidylate synthetase Biotechnology TP248.13-248.65
spellingShingle	lung adenocarcinoma microrna-140-3p proliferation metastasis angiogenesis thymidylate synthetase Biotechnology TP248.13-248.65 Shanzhi Wan Zhimin Liu Yang Chen Zhitao Mai Mingming Jiang Qingguo Di Baohua Sun MicroRNA-140-3p represses the proliferation, migration, invasion and angiogenesis of lung adenocarcinoma cells via targeting TYMS (Thymidylate Synthetase)
description	MicroRNA (miR)-140-3p has been proved to repress lung adenocarcinoma (LUAD), and our study aims to further evaluate the mechanism. Bioinformatic analyses were performed. The viability, proliferation, migration, invasion and angiogenesis of transfected LUAD cells were all determined via Cell Counting Kit-8, colony formation, Scratch, Transwell, and tube formation assays. The targeting relationship between miR-140-3p and Thymidylate Synthetase (TYMS) was confirmed by dual-luciferase reporter (DLR) assay. Relative expressions of miR-140-3p, TYMS, metastasis- (E-Cadherin, N-Cadherin, Vimentin), angiogenesis- (vascular endothelial growth factor (VEGF)), and apoptosis-related factors (cleaved Caspase-3, B-cell lymphoma-2 (Bcl-2), Bcl-2-associated X protein (Bax)) were quantified by quantitative real-time polymerase chain reaction or Western blot. TYMS was high-expressed yet miR-140-3p was low-expressed in LUAD cells. Upregulation of miR-140-3p inhibited TYMS expression, viability, colony formation, migration, invasion, and tube length within LUAD cells, while downregulation of miR-140-3p did oppositely. Silenced TYMS, the downstream target gene of miR-140-3p, reversed the effects of miR-140-3p downregulation on TYMS expression, cell viability, colony formation, migration, invasion, and tube length as well as the metastasis-, apoptosis- and angiogenesis-related proteins in LUAD cells. Upregulation of miR-140-3p inhibited the proliferation, migration, invasion and angiogenesis of LUAD cells via targeting TYMS.
format	article
author	Shanzhi Wan Zhimin Liu Yang Chen Zhitao Mai Mingming Jiang Qingguo Di Baohua Sun
author_facet	Shanzhi Wan Zhimin Liu Yang Chen Zhitao Mai Mingming Jiang Qingguo Di Baohua Sun
author_sort	Shanzhi Wan
title	MicroRNA-140-3p represses the proliferation, migration, invasion and angiogenesis of lung adenocarcinoma cells via targeting TYMS (Thymidylate Synthetase)
title_short	MicroRNA-140-3p represses the proliferation, migration, invasion and angiogenesis of lung adenocarcinoma cells via targeting TYMS (Thymidylate Synthetase)
title_full	MicroRNA-140-3p represses the proliferation, migration, invasion and angiogenesis of lung adenocarcinoma cells via targeting TYMS (Thymidylate Synthetase)
title_fullStr	MicroRNA-140-3p represses the proliferation, migration, invasion and angiogenesis of lung adenocarcinoma cells via targeting TYMS (Thymidylate Synthetase)
title_full_unstemmed	MicroRNA-140-3p represses the proliferation, migration, invasion and angiogenesis of lung adenocarcinoma cells via targeting TYMS (Thymidylate Synthetase)
title_sort	microrna-140-3p represses the proliferation, migration, invasion and angiogenesis of lung adenocarcinoma cells via targeting tyms (thymidylate synthetase)
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/339c2728e55e404388911ee22047dd0e
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MicroRNA-140-3p represses the proliferation, migration, invasion and angiogenesis of lung adenocarcinoma cells via targeting TYMS (Thymidylate Synthetase)

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