Video-rate multi-color structured illumination microscopy with simultaneous real-time reconstruction

Sequential acquisition and image reconstruction in super-resolved structured illumination microscopy (SR-SIM) is time-consuming. Here the authors optimise both acquisition and reconstruction software to achieve multicolour SR-SIM at video frame-rates with reconstructed images displaying with only mi...

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Autores principales: Andreas Markwirth, Mario Lachetta, Viola Mönkemöller, Rainer Heintzmann, Wolfgang Hübner, Thomas Huser, Marcel Müller
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2019
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Acceso en línea:https://doaj.org/article/33ab98ebac7444389260e1e9acdee889
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Sumario:Sequential acquisition and image reconstruction in super-resolved structured illumination microscopy (SR-SIM) is time-consuming. Here the authors optimise both acquisition and reconstruction software to achieve multicolour SR-SIM at video frame-rates with reconstructed images displaying with only milliseconds delay during the experiment.