Video-rate multi-color structured illumination microscopy with simultaneous real-time reconstruction

Sequential acquisition and image reconstruction in super-resolved structured illumination microscopy (SR-SIM) is time-consuming. Here the authors optimise both acquisition and reconstruction software to achieve multicolour SR-SIM at video frame-rates with reconstructed images displaying with only mi...

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Autores principales: Andreas Markwirth, Mario Lachetta, Viola Mönkemöller, Rainer Heintzmann, Wolfgang Hübner, Thomas Huser, Marcel Müller
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2019
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Acceso en línea:https://doaj.org/article/33ab98ebac7444389260e1e9acdee889
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spelling oai:doaj.org-article:33ab98ebac7444389260e1e9acdee8892021-12-02T16:57:10ZVideo-rate multi-color structured illumination microscopy with simultaneous real-time reconstruction10.1038/s41467-019-12165-x2041-1723https://doaj.org/article/33ab98ebac7444389260e1e9acdee8892019-09-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-12165-xhttps://doaj.org/toc/2041-1723Sequential acquisition and image reconstruction in super-resolved structured illumination microscopy (SR-SIM) is time-consuming. Here the authors optimise both acquisition and reconstruction software to achieve multicolour SR-SIM at video frame-rates with reconstructed images displaying with only milliseconds delay during the experiment.Andreas MarkwirthMario LachettaViola MönkemöllerRainer HeintzmannWolfgang HübnerThomas HuserMarcel MüllerNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-11 (2019)
institution DOAJ
collection DOAJ
language EN
topic Science
Q
spellingShingle Science
Q
Andreas Markwirth
Mario Lachetta
Viola Mönkemöller
Rainer Heintzmann
Wolfgang Hübner
Thomas Huser
Marcel Müller
Video-rate multi-color structured illumination microscopy with simultaneous real-time reconstruction
description Sequential acquisition and image reconstruction in super-resolved structured illumination microscopy (SR-SIM) is time-consuming. Here the authors optimise both acquisition and reconstruction software to achieve multicolour SR-SIM at video frame-rates with reconstructed images displaying with only milliseconds delay during the experiment.
format article
author Andreas Markwirth
Mario Lachetta
Viola Mönkemöller
Rainer Heintzmann
Wolfgang Hübner
Thomas Huser
Marcel Müller
author_facet Andreas Markwirth
Mario Lachetta
Viola Mönkemöller
Rainer Heintzmann
Wolfgang Hübner
Thomas Huser
Marcel Müller
author_sort Andreas Markwirth
title Video-rate multi-color structured illumination microscopy with simultaneous real-time reconstruction
title_short Video-rate multi-color structured illumination microscopy with simultaneous real-time reconstruction
title_full Video-rate multi-color structured illumination microscopy with simultaneous real-time reconstruction
title_fullStr Video-rate multi-color structured illumination microscopy with simultaneous real-time reconstruction
title_full_unstemmed Video-rate multi-color structured illumination microscopy with simultaneous real-time reconstruction
title_sort video-rate multi-color structured illumination microscopy with simultaneous real-time reconstruction
publisher Nature Portfolio
publishDate 2019
url https://doaj.org/article/33ab98ebac7444389260e1e9acdee889
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