DNA Barcoding studies on Thrips in India: Cryptic species and Species complexes

Abstract Thrips are one of the major sucking pest and vector of plant viruses causing huge economic loss in agriculture. The accurate identification of thrips is crucial for effective pest management strategies. However, morphology based identification has limitations and warrants integration of mol...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Kaomud Tyagi, Vikas Kumar, Devkant Singha, Kailash Chandra, Boni Amin Laskar, Shantanu Kundu, Rajasree Chakraborty, Sumantika Chatterjee
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/33ac9c1f2abd4d608e1f529f39b2b860
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:33ac9c1f2abd4d608e1f529f39b2b860
record_format	dspace
spelling	oai:doaj.org-article:33ac9c1f2abd4d608e1f529f39b2b8602021-12-02T12:30:13ZDNA Barcoding studies on Thrips in India: Cryptic species and Species complexes10.1038/s41598-017-05112-72045-2322https://doaj.org/article/33ac9c1f2abd4d608e1f529f39b2b8602017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05112-7https://doaj.org/toc/2045-2322Abstract Thrips are one of the major sucking pest and vector of plant viruses causing huge economic loss in agriculture. The accurate identification of thrips is crucial for effective pest management strategies. However, morphology based identification has limitations and warrants integration of molecular data. We attempted the largest DNA barcoding initiative on 370 sequences of 89 thrips morphospecies including 104 novel sequences from 39 morphospecies, including the type specimens of four species. The results of multiple species delimitation methods (BIN, ABGD, GMYC and bPTP) were consistent for 73 species (82%) with their morphological identifications. A total of 107 molecular operational taxonomic units (MOTUs) was recovered for 89 morphospecies by superimposing multiple methods and applying a three level nomenclature system. We detected more than one MOTU in 14 morphospecies indicating to have cryptic diversity including, two major vector species (Frankliniella schultzei and Thrips palmi). However, four morphospecies (Thrips moundi, Thrips carthami, Haplothrips andersi and Haplothrips gowdeyi) showed low genetic distances between them with overlapping in barcode gap that requires further analysis with multiple molecular markers and more specimens from wide geographical areas for better taxonomic judgment. We also presented the advantage of simultaneous use of multiple delimitation methods for detection and identification of cryptic species.Kaomud TyagiVikas KumarDevkant SinghaKailash ChandraBoni Amin LaskarShantanu KunduRajasree ChakrabortySumantika ChatterjeeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kaomud Tyagi Vikas Kumar Devkant Singha Kailash Chandra Boni Amin Laskar Shantanu Kundu Rajasree Chakraborty Sumantika Chatterjee DNA Barcoding studies on Thrips in India: Cryptic species and Species complexes
description	Abstract Thrips are one of the major sucking pest and vector of plant viruses causing huge economic loss in agriculture. The accurate identification of thrips is crucial for effective pest management strategies. However, morphology based identification has limitations and warrants integration of molecular data. We attempted the largest DNA barcoding initiative on 370 sequences of 89 thrips morphospecies including 104 novel sequences from 39 morphospecies, including the type specimens of four species. The results of multiple species delimitation methods (BIN, ABGD, GMYC and bPTP) were consistent for 73 species (82%) with their morphological identifications. A total of 107 molecular operational taxonomic units (MOTUs) was recovered for 89 morphospecies by superimposing multiple methods and applying a three level nomenclature system. We detected more than one MOTU in 14 morphospecies indicating to have cryptic diversity including, two major vector species (Frankliniella schultzei and Thrips palmi). However, four morphospecies (Thrips moundi, Thrips carthami, Haplothrips andersi and Haplothrips gowdeyi) showed low genetic distances between them with overlapping in barcode gap that requires further analysis with multiple molecular markers and more specimens from wide geographical areas for better taxonomic judgment. We also presented the advantage of simultaneous use of multiple delimitation methods for detection and identification of cryptic species.
format	article
author	Kaomud Tyagi Vikas Kumar Devkant Singha Kailash Chandra Boni Amin Laskar Shantanu Kundu Rajasree Chakraborty Sumantika Chatterjee
author_facet	Kaomud Tyagi Vikas Kumar Devkant Singha Kailash Chandra Boni Amin Laskar Shantanu Kundu Rajasree Chakraborty Sumantika Chatterjee
author_sort	Kaomud Tyagi
title	DNA Barcoding studies on Thrips in India: Cryptic species and Species complexes
title_short	DNA Barcoding studies on Thrips in India: Cryptic species and Species complexes
title_full	DNA Barcoding studies on Thrips in India: Cryptic species and Species complexes
title_fullStr	DNA Barcoding studies on Thrips in India: Cryptic species and Species complexes
title_full_unstemmed	DNA Barcoding studies on Thrips in India: Cryptic species and Species complexes
title_sort	dna barcoding studies on thrips in india: cryptic species and species complexes
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/33ac9c1f2abd4d608e1f529f39b2b860
work_keys_str_mv	AT kaomudtyagi dnabarcodingstudiesonthripsinindiacrypticspeciesandspeciescomplexes AT vikaskumar dnabarcodingstudiesonthripsinindiacrypticspeciesandspeciescomplexes AT devkantsingha dnabarcodingstudiesonthripsinindiacrypticspeciesandspeciescomplexes AT kailashchandra dnabarcodingstudiesonthripsinindiacrypticspeciesandspeciescomplexes AT boniaminlaskar dnabarcodingstudiesonthripsinindiacrypticspeciesandspeciescomplexes AT shantanukundu dnabarcodingstudiesonthripsinindiacrypticspeciesandspeciescomplexes AT rajasreechakraborty dnabarcodingstudiesonthripsinindiacrypticspeciesandspeciescomplexes AT sumantikachatterjee dnabarcodingstudiesonthripsinindiacrypticspeciesandspeciescomplexes
_version_	1718394390160867328

DNA Barcoding studies on Thrips in India: Cryptic species and Species complexes

Ejemplares similares