Modeling undernutrition with enteropathy in mice

Abstract Undernutrition is a global health issue leading to 1 out 5 all deaths in children under 5 years. Undernutrition is often associated with environmental enteric dysfunction (EED), a syndrome associated with increased intestinal permeability and gut inflammation. We aimed to develop a novel mu...

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Autores principales:	Emmeline Salameh, Marine Jarbeau, Fanny B. Morel, Mamane Zeilani, Moutaz Aziz, Pierre Déchelotte, Rachel Marion-Letellier
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/33b32b979bd0443bbf98f2a5a612b9c5
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spelling	oai:doaj.org-article:33b32b979bd0443bbf98f2a5a612b9c52021-12-02T18:48:01ZModeling undernutrition with enteropathy in mice10.1038/s41598-020-72705-02045-2322https://doaj.org/article/33b32b979bd0443bbf98f2a5a612b9c52020-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-72705-0https://doaj.org/toc/2045-2322Abstract Undernutrition is a global health issue leading to 1 out 5 all deaths in children under 5 years. Undernutrition is often associated with environmental enteric dysfunction (EED), a syndrome associated with increased intestinal permeability and gut inflammation. We aimed to develop a novel murine model of undernutrition with these EED features. Post-weaning mice were fed with low-protein diet (LP) alone or combined with a gastrointestinal insult trigger (indomethacin or liposaccharides). Growth, intestinal permeability and inflammation were assessed. LP diet induced stunting and wasting in post-weaning mice but did not impact gut barrier. We therefore combined LP diet with a single administration of indomethacin or liposaccharides (LPS). Indomethacin increased fecal calprotectin production while LPS did not. To amplify indomethacin effects, we investigated its repeated administration in addition to LP diet and mice exhibited stunting and wasting with intestinal hyperpermeability and gut inflammation. The combination of 3-weeks LP diet with repeated oral indomethacin administration induced wasting, stunting and gut barrier dysfunction as observed in undernourished children with EED. As noninvasive methods for investigating gut function in undernourished children are scarce, the present pre-clinical model provides an affordable tool to attempt to elucidate pathophysiological processes involved in EED and to identify novel therapeutic strategies.Emmeline SalamehMarine JarbeauFanny B. MorelMamane ZeilaniMoutaz AzizPierre DéchelotteRachel Marion-LetellierNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-15 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Emmeline Salameh Marine Jarbeau Fanny B. Morel Mamane Zeilani Moutaz Aziz Pierre Déchelotte Rachel Marion-Letellier Modeling undernutrition with enteropathy in mice
description	Abstract Undernutrition is a global health issue leading to 1 out 5 all deaths in children under 5 years. Undernutrition is often associated with environmental enteric dysfunction (EED), a syndrome associated with increased intestinal permeability and gut inflammation. We aimed to develop a novel murine model of undernutrition with these EED features. Post-weaning mice were fed with low-protein diet (LP) alone or combined with a gastrointestinal insult trigger (indomethacin or liposaccharides). Growth, intestinal permeability and inflammation were assessed. LP diet induced stunting and wasting in post-weaning mice but did not impact gut barrier. We therefore combined LP diet with a single administration of indomethacin or liposaccharides (LPS). Indomethacin increased fecal calprotectin production while LPS did not. To amplify indomethacin effects, we investigated its repeated administration in addition to LP diet and mice exhibited stunting and wasting with intestinal hyperpermeability and gut inflammation. The combination of 3-weeks LP diet with repeated oral indomethacin administration induced wasting, stunting and gut barrier dysfunction as observed in undernourished children with EED. As noninvasive methods for investigating gut function in undernourished children are scarce, the present pre-clinical model provides an affordable tool to attempt to elucidate pathophysiological processes involved in EED and to identify novel therapeutic strategies.
format	article
author	Emmeline Salameh Marine Jarbeau Fanny B. Morel Mamane Zeilani Moutaz Aziz Pierre Déchelotte Rachel Marion-Letellier
author_facet	Emmeline Salameh Marine Jarbeau Fanny B. Morel Mamane Zeilani Moutaz Aziz Pierre Déchelotte Rachel Marion-Letellier
author_sort	Emmeline Salameh
title	Modeling undernutrition with enteropathy in mice
title_short	Modeling undernutrition with enteropathy in mice
title_full	Modeling undernutrition with enteropathy in mice
title_fullStr	Modeling undernutrition with enteropathy in mice
title_full_unstemmed	Modeling undernutrition with enteropathy in mice
title_sort	modeling undernutrition with enteropathy in mice
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/33b32b979bd0443bbf98f2a5a612b9c5
work_keys_str_mv	AT emmelinesalameh modelingundernutritionwithenteropathyinmice AT marinejarbeau modelingundernutritionwithenteropathyinmice AT fannybmorel modelingundernutritionwithenteropathyinmice AT mamanezeilani modelingundernutritionwithenteropathyinmice AT moutazaziz modelingundernutritionwithenteropathyinmice AT pierredechelotte modelingundernutritionwithenteropathyinmice AT rachelmarionletellier modelingundernutritionwithenteropathyinmice
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Modeling undernutrition with enteropathy in mice

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