New approaches for the characterization of plastic-associated microbial communities and the discovery of plastic-degrading microorganisms and enzymes

Plastics in the environment represent new substrates for microbial colonization, and recent methodological advances allow for in-depth characterization of plastic-associated microbial communities (PAMCs). Over the past several decades, discovery of plastic degrading enzymes (PDEs) and plastic degrad...

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Autores principales:	V.R. Viljakainen, L.A. Hug
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Plastic degradation Multi-omics Plastic degrading enzyme Plastic degrading microbe Plastisphere Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/33b92a7822004c12b9bf50764b9e606f
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spelling	oai:doaj.org-article:33b92a7822004c12b9bf50764b9e606f2021-11-26T04:26:53ZNew approaches for the characterization of plastic-associated microbial communities and the discovery of plastic-degrading microorganisms and enzymes2001-037010.1016/j.csbj.2021.11.023https://doaj.org/article/33b92a7822004c12b9bf50764b9e606f2021-01-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2001037021004888https://doaj.org/toc/2001-0370Plastics in the environment represent new substrates for microbial colonization, and recent methodological advances allow for in-depth characterization of plastic-associated microbial communities (PAMCs). Over the past several decades, discovery of plastic degrading enzymes (PDEs) and plastic degrading microorganisms (PDMs) has been driven by efforts to understand microbially-mediated plastic degradation in the environment and to discover biocatalysts for plastic processing. In this review, we discuss the evolution of methodology in plastic microbiology and highlight major advancements in the field stemming from computational microbiology. Initial research relied largely on culture-based approaches like clear-zone assays to screen for PDMs and microscopy to characterize PAMCs. New computational tools and sequencing technologies are accelerating discoveries in the field through culture-independent and multi-omic approaches, rapidly generating targets for protein engineering and improving the potential for plastic-waste management.V.R. ViljakainenL.A. HugElsevierarticlePlastic degradationMulti-omicsPlastic degrading enzymePlastic degrading microbePlastisphereBiotechnologyTP248.13-248.65ENComputational and Structural Biotechnology Journal, Vol 19, Iss , Pp 6191-6200 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Plastic degradation Multi-omics Plastic degrading enzyme Plastic degrading microbe Plastisphere Biotechnology TP248.13-248.65
spellingShingle	Plastic degradation Multi-omics Plastic degrading enzyme Plastic degrading microbe Plastisphere Biotechnology TP248.13-248.65 V.R. Viljakainen L.A. Hug New approaches for the characterization of plastic-associated microbial communities and the discovery of plastic-degrading microorganisms and enzymes
description	Plastics in the environment represent new substrates for microbial colonization, and recent methodological advances allow for in-depth characterization of plastic-associated microbial communities (PAMCs). Over the past several decades, discovery of plastic degrading enzymes (PDEs) and plastic degrading microorganisms (PDMs) has been driven by efforts to understand microbially-mediated plastic degradation in the environment and to discover biocatalysts for plastic processing. In this review, we discuss the evolution of methodology in plastic microbiology and highlight major advancements in the field stemming from computational microbiology. Initial research relied largely on culture-based approaches like clear-zone assays to screen for PDMs and microscopy to characterize PAMCs. New computational tools and sequencing technologies are accelerating discoveries in the field through culture-independent and multi-omic approaches, rapidly generating targets for protein engineering and improving the potential for plastic-waste management.
format	article
author	V.R. Viljakainen L.A. Hug
author_facet	V.R. Viljakainen L.A. Hug
author_sort	V.R. Viljakainen
title	New approaches for the characterization of plastic-associated microbial communities and the discovery of plastic-degrading microorganisms and enzymes
title_short	New approaches for the characterization of plastic-associated microbial communities and the discovery of plastic-degrading microorganisms and enzymes
title_full	New approaches for the characterization of plastic-associated microbial communities and the discovery of plastic-degrading microorganisms and enzymes
title_fullStr	New approaches for the characterization of plastic-associated microbial communities and the discovery of plastic-degrading microorganisms and enzymes
title_full_unstemmed	New approaches for the characterization of plastic-associated microbial communities and the discovery of plastic-degrading microorganisms and enzymes
title_sort	new approaches for the characterization of plastic-associated microbial communities and the discovery of plastic-degrading microorganisms and enzymes
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/33b92a7822004c12b9bf50764b9e606f
work_keys_str_mv	AT vrviljakainen newapproachesforthecharacterizationofplasticassociatedmicrobialcommunitiesandthediscoveryofplasticdegradingmicroorganismsandenzymes AT lahug newapproachesforthecharacterizationofplasticassociatedmicrobialcommunitiesandthediscoveryofplasticdegradingmicroorganismsandenzymes
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New approaches for the characterization of plastic-associated microbial communities and the discovery of plastic-degrading microorganisms and enzymes

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