Ultrasmall superparamagnetic nanoparticles targeting E-selectin: synthesis and effects in mice in vitro and in vivo

Lijuan Liu,* Lu Liu,* Yin Li, Xiaoxin Huang, Donglian Gu, Bo Wei, Danke Su, Guanqiao JinCentre of Imaging Diagnosis, Affiliated Tumor Hospital of Guangxi Medical University, Nanning, Guangxi, People’s Republic of China*These authors contributed equally to this work Purpose: We developed a...

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Autores principales:	Liu L, Li Y, Huang X, Gu D, Wei B, Su D, Jin G
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	USPIO E-selectin molecular imaging nasopharyngeal carcinoma MR imaging Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/33c28f3b641a42d8904861c70b44c5e1
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spelling	oai:doaj.org-article:33c28f3b641a42d8904861c70b44c5e12021-12-02T06:41:28ZUltrasmall superparamagnetic nanoparticles targeting E-selectin: synthesis and effects in mice in vitro and in vivo1178-2013https://doaj.org/article/33c28f3b641a42d8904861c70b44c5e12019-06-01T00:00:00Zhttps://www.dovepress.com/ultrasmall-superparamagnetic-nanoparticles-targeting-e-selectin-synthe-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Lijuan Liu,* Lu Liu,* Yin Li, Xiaoxin Huang, Donglian Gu, Bo Wei, Danke Su, Guanqiao JinCentre of Imaging Diagnosis, Affiliated Tumor Hospital of Guangxi Medical University, Nanning, Guangxi, People’s Republic of ChinaThese authors contributed equally to this work Purpose: We developed a contrast agent for targeting E-selectin expression. We detected the agent using magnetic resonance imaging (MRI) in vivo in nude mice that had undergone nasopharyngeal carcinoma (NPC) metastasis.Methods: Sialyl Lewis X (sLeX) was conjugated with ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles. Hydrodynamic size, polydispersity index, and ζ-potential of USPIO–polyethylene glycol (PEG) nanoparticles and USPIO-PEG-sLeX nanoparticles were measured. Component changes in nanoparticles of USPIO, USPIO-PEG, and USPIO-PEG-sLeX were analyzed by thermogravimetric analysis and Fourier-transform infrared spectroscopy. A model of NPC metastasis to inguinal lymph nodes in nude mice was used to investigate characteristics of the USPIO-PEG-sLeX nanoparticles in vivo. We investigated the ability of the T2 value, change in T2* value (ΔT2* value), and enhancement rate (ER) to assess accumulation of USPIO-PEG-sLeX nanoparticles quantitatively in mice of a metastasis group and control group. Four MRI scans were undertaken for each mouse. The first scan (t0) was done before administration of USPIO-PEG-sLeX nanoparticles (0.1 mL) via the tail vein. The other scans were carried out at 0 (t1), 1 (t2), and 2 hours (t3) postinjection. The mean optical density was used to reflect E-selectin expression.Results: sLeX was labeled onto USPIO successfully. In vivo, there were significant interactions between the groups and time for T2* values after administration of USPIO-PEG-sLeX nanoparticles. Six parameters (T2* at t2, ΔT2* at t1, ΔT2* at t2, ER at t1, ER at t2, and ER at t3) were correlated with the mean optical density.Conclusion: USPIO-PEG-sLeX nanoparticles can be used to assess E-selectin expression quantitatively. Use of such molecular probes could enable detection of early metastasis of NPC, more accurate staging, and treatment monitoring.Keywords: molecular MRI, contrast agent, E-selectin, Sialyl Lewis X, nasopharyngeal carcinoma, iron oxideLiu LLiu LLi YHuang XGu DWei BSu DJin GDove Medical PressarticleUSPIOE-selectinmolecular imagingnasopharyngeal carcinomaMR imagingMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 4517-4528 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	USPIO E-selectin molecular imaging nasopharyngeal carcinoma MR imaging Medicine (General) R5-920
spellingShingle	USPIO E-selectin molecular imaging nasopharyngeal carcinoma MR imaging Medicine (General) R5-920 Liu L Liu L Li Y Huang X Gu D Wei B Su D Jin G Ultrasmall superparamagnetic nanoparticles targeting E-selectin: synthesis and effects in mice in vitro and in vivo
description	Lijuan Liu,* Lu Liu,* Yin Li, Xiaoxin Huang, Donglian Gu, Bo Wei, Danke Su, Guanqiao JinCentre of Imaging Diagnosis, Affiliated Tumor Hospital of Guangxi Medical University, Nanning, Guangxi, People’s Republic of ChinaThese authors contributed equally to this work Purpose: We developed a contrast agent for targeting E-selectin expression. We detected the agent using magnetic resonance imaging (MRI) in vivo in nude mice that had undergone nasopharyngeal carcinoma (NPC) metastasis.Methods: Sialyl Lewis X (sLeX) was conjugated with ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles. Hydrodynamic size, polydispersity index, and ζ-potential of USPIO–polyethylene glycol (PEG) nanoparticles and USPIO-PEG-sLeX nanoparticles were measured. Component changes in nanoparticles of USPIO, USPIO-PEG, and USPIO-PEG-sLeX were analyzed by thermogravimetric analysis and Fourier-transform infrared spectroscopy. A model of NPC metastasis to inguinal lymph nodes in nude mice was used to investigate characteristics of the USPIO-PEG-sLeX nanoparticles in vivo. We investigated the ability of the T2 value, change in T2* value (ΔT2* value), and enhancement rate (ER) to assess accumulation of USPIO-PEG-sLeX nanoparticles quantitatively in mice of a metastasis group and control group. Four MRI scans were undertaken for each mouse. The first scan (t0) was done before administration of USPIO-PEG-sLeX nanoparticles (0.1 mL) via the tail vein. The other scans were carried out at 0 (t1), 1 (t2), and 2 hours (t3) postinjection. The mean optical density was used to reflect E-selectin expression.Results: sLeX was labeled onto USPIO successfully. In vivo, there were significant interactions between the groups and time for T2* values after administration of USPIO-PEG-sLeX nanoparticles. Six parameters (T2* at t2, ΔT2* at t1, ΔT2* at t2, ER at t1, ER at t2, and ER at t3) were correlated with the mean optical density.Conclusion: USPIO-PEG-sLeX nanoparticles can be used to assess E-selectin expression quantitatively. Use of such molecular probes could enable detection of early metastasis of NPC, more accurate staging, and treatment monitoring.Keywords: molecular MRI, contrast agent, E-selectin, Sialyl Lewis X, nasopharyngeal carcinoma, iron oxide
format	article
author	Liu L Liu L Li Y Huang X Gu D Wei B Su D Jin G
author_facet	Liu L Liu L Li Y Huang X Gu D Wei B Su D Jin G
author_sort	Liu L
title	Ultrasmall superparamagnetic nanoparticles targeting E-selectin: synthesis and effects in mice in vitro and in vivo
title_short	Ultrasmall superparamagnetic nanoparticles targeting E-selectin: synthesis and effects in mice in vitro and in vivo
title_full	Ultrasmall superparamagnetic nanoparticles targeting E-selectin: synthesis and effects in mice in vitro and in vivo
title_fullStr	Ultrasmall superparamagnetic nanoparticles targeting E-selectin: synthesis and effects in mice in vitro and in vivo
title_full_unstemmed	Ultrasmall superparamagnetic nanoparticles targeting E-selectin: synthesis and effects in mice in vitro and in vivo
title_sort	ultrasmall superparamagnetic nanoparticles targeting e-selectin: synthesis and effects in mice in vitro and in vivo
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/33c28f3b641a42d8904861c70b44c5e1
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Ultrasmall superparamagnetic nanoparticles targeting E-selectin: synthesis and effects in mice in vitro and in vivo

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