Satellite cells senescence in limb muscle of severe patients with COPD.

<h4>Rationale</h4>The maintenance of peripheral muscle mass may be compromised in chronic obstructive pulmonary disease (COPD) due to premature cellular senescence and exhaustion of the regenerative potential of the muscles.<h4>Methods</h4>Vastus lateralis biopsies were obtai...

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Autores principales:	Marie-Eve Thériault, Marie-Ève Paré, François Maltais, Richard Debigaré
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/33c50c8a58ff4bc89bb0dccce3c1a558
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spelling	oai:doaj.org-article:33c50c8a58ff4bc89bb0dccce3c1a5582021-11-18T07:15:33ZSatellite cells senescence in limb muscle of severe patients with COPD.1932-620310.1371/journal.pone.0039124https://doaj.org/article/33c50c8a58ff4bc89bb0dccce3c1a5582012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22720047/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Rationale</h4>The maintenance of peripheral muscle mass may be compromised in chronic obstructive pulmonary disease (COPD) due to premature cellular senescence and exhaustion of the regenerative potential of the muscles.<h4>Methods</h4>Vastus lateralis biopsies were obtained from patients with COPD (n = 16) and healthy subjects (n = 7). Satellite cell number and the proportion of central nuclei, as a marker of muscle regenerative events, were assessed on cryosections. Telomere lengths, used as a marker of cellular senescence, were determined using Southern blot analyses.<h4>Results</h4>Central nuclei proportion was significantly higher in patients with COPD with a preserved muscle mass compared to controls and patients with COPD with muscle atrophy (p<0.001). In COPD, maximal telomere length was significantly decreased compared to controls (p<0.05). Similarly, minimal telomere length was significantly reduced in GOLD III-IV patients with muscle atrophy compared to controls (p<0.005). Minimal, mean and maximum telomere lengths correlated with mid-thigh muscle cross-sectional area (MTCSA) (R = 0.523, p = 0.005; R = 0.435, p = 0.019 and R = 0.491, p = 0.009, respectively).<h4>Conclusions</h4>Evidence of increased regenerative events was seen in GOLD III-IV patients with preserved muscle mass. Shortening of telomeres in GOLD III-IV patients with muscle atrophy is consistent with an increased number of senescent satellite cells and an exhausted muscle regenerative capacity, compromising the maintenance of muscle mass in these individuals.Marie-Eve ThériaultMarie-Ève ParéFrançois MaltaisRichard DebigaréPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 6, p e39124 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Marie-Eve Thériault Marie-Ève Paré François Maltais Richard Debigaré Satellite cells senescence in limb muscle of severe patients with COPD.
description	<h4>Rationale</h4>The maintenance of peripheral muscle mass may be compromised in chronic obstructive pulmonary disease (COPD) due to premature cellular senescence and exhaustion of the regenerative potential of the muscles.<h4>Methods</h4>Vastus lateralis biopsies were obtained from patients with COPD (n = 16) and healthy subjects (n = 7). Satellite cell number and the proportion of central nuclei, as a marker of muscle regenerative events, were assessed on cryosections. Telomere lengths, used as a marker of cellular senescence, were determined using Southern blot analyses.<h4>Results</h4>Central nuclei proportion was significantly higher in patients with COPD with a preserved muscle mass compared to controls and patients with COPD with muscle atrophy (p<0.001). In COPD, maximal telomere length was significantly decreased compared to controls (p<0.05). Similarly, minimal telomere length was significantly reduced in GOLD III-IV patients with muscle atrophy compared to controls (p<0.005). Minimal, mean and maximum telomere lengths correlated with mid-thigh muscle cross-sectional area (MTCSA) (R = 0.523, p = 0.005; R = 0.435, p = 0.019 and R = 0.491, p = 0.009, respectively).<h4>Conclusions</h4>Evidence of increased regenerative events was seen in GOLD III-IV patients with preserved muscle mass. Shortening of telomeres in GOLD III-IV patients with muscle atrophy is consistent with an increased number of senescent satellite cells and an exhausted muscle regenerative capacity, compromising the maintenance of muscle mass in these individuals.
format	article
author	Marie-Eve Thériault Marie-Ève Paré François Maltais Richard Debigaré
author_facet	Marie-Eve Thériault Marie-Ève Paré François Maltais Richard Debigaré
author_sort	Marie-Eve Thériault
title	Satellite cells senescence in limb muscle of severe patients with COPD.
title_short	Satellite cells senescence in limb muscle of severe patients with COPD.
title_full	Satellite cells senescence in limb muscle of severe patients with COPD.
title_fullStr	Satellite cells senescence in limb muscle of severe patients with COPD.
title_full_unstemmed	Satellite cells senescence in limb muscle of severe patients with COPD.
title_sort	satellite cells senescence in limb muscle of severe patients with copd.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/33c50c8a58ff4bc89bb0dccce3c1a558
work_keys_str_mv	AT marieevetheriault satellitecellssenescenceinlimbmuscleofseverepatientswithcopd AT marieevepare satellitecellssenescenceinlimbmuscleofseverepatientswithcopd AT francoismaltais satellitecellssenescenceinlimbmuscleofseverepatientswithcopd AT richarddebigare satellitecellssenescenceinlimbmuscleofseverepatientswithcopd
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Satellite cells senescence in limb muscle of severe patients with COPD.

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