A proteomic analysis of an in vitro knock-out of miR-200c

Abstract Loss of miR-200c is correlated to advanced cancer-subtypes due to increased EMT and decreased treatment efficacy by chemotherapeutics. As miRNAs regulate a multitude of targets, the analysis of differentially expressed proteins upon a genomic knock-out (KO) is of interest. In this study, we...

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Autores principales:	Bojan Ljepoja, Jonathan García-Roman, Ann-Katrin Sommer, Thomas Fröhlich, Georg J. Arnold, Ernst Wagner, Andreas Roidl
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/33c8ee14553a400c8741f1a8882b0995
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spelling	oai:doaj.org-article:33c8ee14553a400c8741f1a8882b09952021-12-02T12:32:35ZA proteomic analysis of an in vitro knock-out of miR-200c10.1038/s41598-018-25240-y2045-2322https://doaj.org/article/33c8ee14553a400c8741f1a8882b09952018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-25240-yhttps://doaj.org/toc/2045-2322Abstract Loss of miR-200c is correlated to advanced cancer-subtypes due to increased EMT and decreased treatment efficacy by chemotherapeutics. As miRNAs regulate a multitude of targets, the analysis of differentially expressed proteins upon a genomic knock-out (KO) is of interest. In this study, we generated a TALENs KO of miR-200c in MCF7 breast cancer cells, excluded its compensation by family-members and evaluated the impact on the proteome by analyzing three individual KO-clones. We identified 26 key proteins and a variety of enrichments in metabolic and cytoskeletal pathways. In six of these targets (AGR2, FLNA/B, ALDH7A1, SCIN, GSTM3) the differential expression was additionally detected at mRNA level. Together, these alterations in protein abundance accounted for the observed biological phenotypes, i.e. increased migration and chemoresistance and altered metabolism, found in the miR-200c-KO clones. These findings provide novel insights into miR-200c and pave the way for further studies.Bojan LjepojaJonathan García-RomanAnn-Katrin SommerThomas FröhlichGeorg J. ArnoldErnst WagnerAndreas RoidlNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-15 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Bojan Ljepoja Jonathan García-Roman Ann-Katrin Sommer Thomas Fröhlich Georg J. Arnold Ernst Wagner Andreas Roidl A proteomic analysis of an in vitro knock-out of miR-200c
description	Abstract Loss of miR-200c is correlated to advanced cancer-subtypes due to increased EMT and decreased treatment efficacy by chemotherapeutics. As miRNAs regulate a multitude of targets, the analysis of differentially expressed proteins upon a genomic knock-out (KO) is of interest. In this study, we generated a TALENs KO of miR-200c in MCF7 breast cancer cells, excluded its compensation by family-members and evaluated the impact on the proteome by analyzing three individual KO-clones. We identified 26 key proteins and a variety of enrichments in metabolic and cytoskeletal pathways. In six of these targets (AGR2, FLNA/B, ALDH7A1, SCIN, GSTM3) the differential expression was additionally detected at mRNA level. Together, these alterations in protein abundance accounted for the observed biological phenotypes, i.e. increased migration and chemoresistance and altered metabolism, found in the miR-200c-KO clones. These findings provide novel insights into miR-200c and pave the way for further studies.
format	article
author	Bojan Ljepoja Jonathan García-Roman Ann-Katrin Sommer Thomas Fröhlich Georg J. Arnold Ernst Wagner Andreas Roidl
author_facet	Bojan Ljepoja Jonathan García-Roman Ann-Katrin Sommer Thomas Fröhlich Georg J. Arnold Ernst Wagner Andreas Roidl
author_sort	Bojan Ljepoja
title	A proteomic analysis of an in vitro knock-out of miR-200c
title_short	A proteomic analysis of an in vitro knock-out of miR-200c
title_full	A proteomic analysis of an in vitro knock-out of miR-200c
title_fullStr	A proteomic analysis of an in vitro knock-out of miR-200c
title_full_unstemmed	A proteomic analysis of an in vitro knock-out of miR-200c
title_sort	proteomic analysis of an in vitro knock-out of mir-200c
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/33c8ee14553a400c8741f1a8882b0995
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A proteomic analysis of an in vitro knock-out of miR-200c

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