Development and validation of a real-time PCR for Chlamydia suis diagnosis in swine and humans.

Pigs are the natural host for Chlamydia suis, a pathogen which is phylogenetically highly related to the human pathogen C. trachomatis. Chlamydia suis infections are generally treated with tetracyclines. In 1998, tetracyline resistant C. suis strains emerged on U.S. pig farms and they are currently...

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Autores principales:	Kristien De Puysseleyr, Leentje De Puysseleyr, Julie Geldhof, Eric Cox, Daisy Vanrompay
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/33ce6449bc95431ea0d091be8ac07f37
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spelling	oai:doaj.org-article:33ce6449bc95431ea0d091be8ac07f372021-11-18T08:20:02ZDevelopment and validation of a real-time PCR for Chlamydia suis diagnosis in swine and humans.1932-620310.1371/journal.pone.0096704https://doaj.org/article/33ce6449bc95431ea0d091be8ac07f372014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24816542/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Pigs are the natural host for Chlamydia suis, a pathogen which is phylogenetically highly related to the human pathogen C. trachomatis. Chlamydia suis infections are generally treated with tetracyclines. In 1998, tetracyline resistant C. suis strains emerged on U.S. pig farms and they are currently present in the Belgian, Cypriote, German, Israeli, Italian and Swiss pig industry. Infections with tetracycline resistant C. suis strains are mainly associated with severe reproductive failure leading to marked economical loss. We developed a sensitive and specific TaqMan probe-based C. suis real-time PCR for examining clinical samples of both pigs and humans. The analytical sensitivity of the real-time PCR is 10 rDNA copies/reaction without cross-amplifying DNA of other Chlamydia species. The PCR was successfully validated using conjunctival, pharyngeal and stool samples of slaughterhouse employees, as well as porcine samples from two farms with evidence of reproductive failure and one farm without clinical disease. Chlamydia suis was only detected in diseased pigs and in the eyes of humans. Positive humans had no clinical complaints. PCR results were confirmed by culture in McCoy cells. In addition, Chlamydia suis isolates were also examined by the tet(C) PCR, designed for demonstrating the tetracycline resistance gene tet(C). The tet(C) gene was only present in porcine C. suis isolates.Kristien De PuysseleyrLeentje De PuysseleyrJulie GeldhofEric CoxDaisy VanrompayPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 5, p e96704 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kristien De Puysseleyr Leentje De Puysseleyr Julie Geldhof Eric Cox Daisy Vanrompay Development and validation of a real-time PCR for Chlamydia suis diagnosis in swine and humans.
description	Pigs are the natural host for Chlamydia suis, a pathogen which is phylogenetically highly related to the human pathogen C. trachomatis. Chlamydia suis infections are generally treated with tetracyclines. In 1998, tetracyline resistant C. suis strains emerged on U.S. pig farms and they are currently present in the Belgian, Cypriote, German, Israeli, Italian and Swiss pig industry. Infections with tetracycline resistant C. suis strains are mainly associated with severe reproductive failure leading to marked economical loss. We developed a sensitive and specific TaqMan probe-based C. suis real-time PCR for examining clinical samples of both pigs and humans. The analytical sensitivity of the real-time PCR is 10 rDNA copies/reaction without cross-amplifying DNA of other Chlamydia species. The PCR was successfully validated using conjunctival, pharyngeal and stool samples of slaughterhouse employees, as well as porcine samples from two farms with evidence of reproductive failure and one farm without clinical disease. Chlamydia suis was only detected in diseased pigs and in the eyes of humans. Positive humans had no clinical complaints. PCR results were confirmed by culture in McCoy cells. In addition, Chlamydia suis isolates were also examined by the tet(C) PCR, designed for demonstrating the tetracycline resistance gene tet(C). The tet(C) gene was only present in porcine C. suis isolates.
format	article
author	Kristien De Puysseleyr Leentje De Puysseleyr Julie Geldhof Eric Cox Daisy Vanrompay
author_facet	Kristien De Puysseleyr Leentje De Puysseleyr Julie Geldhof Eric Cox Daisy Vanrompay
author_sort	Kristien De Puysseleyr
title	Development and validation of a real-time PCR for Chlamydia suis diagnosis in swine and humans.
title_short	Development and validation of a real-time PCR for Chlamydia suis diagnosis in swine and humans.
title_full	Development and validation of a real-time PCR for Chlamydia suis diagnosis in swine and humans.
title_fullStr	Development and validation of a real-time PCR for Chlamydia suis diagnosis in swine and humans.
title_full_unstemmed	Development and validation of a real-time PCR for Chlamydia suis diagnosis in swine and humans.
title_sort	development and validation of a real-time pcr for chlamydia suis diagnosis in swine and humans.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/33ce6449bc95431ea0d091be8ac07f37
work_keys_str_mv	AT kristiendepuysseleyr developmentandvalidationofarealtimepcrforchlamydiasuisdiagnosisinswineandhumans AT leentjedepuysseleyr developmentandvalidationofarealtimepcrforchlamydiasuisdiagnosisinswineandhumans AT juliegeldhof developmentandvalidationofarealtimepcrforchlamydiasuisdiagnosisinswineandhumans AT ericcox developmentandvalidationofarealtimepcrforchlamydiasuisdiagnosisinswineandhumans AT daisyvanrompay developmentandvalidationofarealtimepcrforchlamydiasuisdiagnosisinswineandhumans
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Development and validation of a real-time PCR for Chlamydia suis diagnosis in swine and humans.

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