Up-regulation of BRCA1-associated RING Domain 1 Promotes Hepatocellular Carcinoma Progression by Targeting Akt Signaling

Abstract The present study was designed to investigate the potential clinical, pathological, prognostic value, role and mechanism of BRCA1-associated RING Domain 1 (BARD1) in Hepatocellular carcinoma (HCC). Quantitative real-time PCR and immunohistochemistry were performed to evaluate the expression...

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Autores principales:	Yan Liao, Shengguang Yuan, Xinhuang Chen, Pengpeng Zhu, Jun Li, Liling Qin, Weijia Liao
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/33cf714f74d24c2f8e600a36f69d5bc3
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spelling	oai:doaj.org-article:33cf714f74d24c2f8e600a36f69d5bc32021-12-02T16:06:46ZUp-regulation of BRCA1-associated RING Domain 1 Promotes Hepatocellular Carcinoma Progression by Targeting Akt Signaling10.1038/s41598-017-07962-72045-2322https://doaj.org/article/33cf714f74d24c2f8e600a36f69d5bc32017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07962-7https://doaj.org/toc/2045-2322Abstract The present study was designed to investigate the potential clinical, pathological, prognostic value, role and mechanism of BRCA1-associated RING Domain 1 (BARD1) in Hepatocellular carcinoma (HCC). Quantitative real-time PCR and immunohistochemistry were performed to evaluate the expression of BARD1 mRNA and protein. The expression of BARD1 in the HCC tissue samples was markedly higher than that in the adjacent noncancerous liver tissues. Elevated BARD1 expression was positively correlated with tumor-node-metastasis stage, Barcelona-Clinic Liver Cancer stage, hepatitis B surface antigen, large tumor size, serum alpha-fetoprotein levels, and serum aspartate aminotransferase levels. Univariate and multivariate analyses revealed the BARD1 was an independent predictor for decreased progression-free survival and overall survival in HCC. In vitro experiments demonstrated that knocking down BARD1 significantly inhibited the proliferation, invasion and migration of HCC cells. Moreover, silencing BARD1 inhibit the signaling pathway via decreased the levels of Akt, mTOR, and MMP-9 and inhibited the phosphorylation of Akt (Ser473) and mTOR (Ser2248). Collectively, our findings suggest that BARD1 may be a novel diagnostic and prognostic biomarker of HCC, and up-regulation of BARD1 can contribute to HCC progression by targeting Akt signaling.Yan LiaoShengguang YuanXinhuang ChenPengpeng ZhuJun LiLiling QinWeijia LiaoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yan Liao Shengguang Yuan Xinhuang Chen Pengpeng Zhu Jun Li Liling Qin Weijia Liao Up-regulation of BRCA1-associated RING Domain 1 Promotes Hepatocellular Carcinoma Progression by Targeting Akt Signaling
description	Abstract The present study was designed to investigate the potential clinical, pathological, prognostic value, role and mechanism of BRCA1-associated RING Domain 1 (BARD1) in Hepatocellular carcinoma (HCC). Quantitative real-time PCR and immunohistochemistry were performed to evaluate the expression of BARD1 mRNA and protein. The expression of BARD1 in the HCC tissue samples was markedly higher than that in the adjacent noncancerous liver tissues. Elevated BARD1 expression was positively correlated with tumor-node-metastasis stage, Barcelona-Clinic Liver Cancer stage, hepatitis B surface antigen, large tumor size, serum alpha-fetoprotein levels, and serum aspartate aminotransferase levels. Univariate and multivariate analyses revealed the BARD1 was an independent predictor for decreased progression-free survival and overall survival in HCC. In vitro experiments demonstrated that knocking down BARD1 significantly inhibited the proliferation, invasion and migration of HCC cells. Moreover, silencing BARD1 inhibit the signaling pathway via decreased the levels of Akt, mTOR, and MMP-9 and inhibited the phosphorylation of Akt (Ser473) and mTOR (Ser2248). Collectively, our findings suggest that BARD1 may be a novel diagnostic and prognostic biomarker of HCC, and up-regulation of BARD1 can contribute to HCC progression by targeting Akt signaling.
format	article
author	Yan Liao Shengguang Yuan Xinhuang Chen Pengpeng Zhu Jun Li Liling Qin Weijia Liao
author_facet	Yan Liao Shengguang Yuan Xinhuang Chen Pengpeng Zhu Jun Li Liling Qin Weijia Liao
author_sort	Yan Liao
title	Up-regulation of BRCA1-associated RING Domain 1 Promotes Hepatocellular Carcinoma Progression by Targeting Akt Signaling
title_short	Up-regulation of BRCA1-associated RING Domain 1 Promotes Hepatocellular Carcinoma Progression by Targeting Akt Signaling
title_full	Up-regulation of BRCA1-associated RING Domain 1 Promotes Hepatocellular Carcinoma Progression by Targeting Akt Signaling
title_fullStr	Up-regulation of BRCA1-associated RING Domain 1 Promotes Hepatocellular Carcinoma Progression by Targeting Akt Signaling
title_full_unstemmed	Up-regulation of BRCA1-associated RING Domain 1 Promotes Hepatocellular Carcinoma Progression by Targeting Akt Signaling
title_sort	up-regulation of brca1-associated ring domain 1 promotes hepatocellular carcinoma progression by targeting akt signaling
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/33cf714f74d24c2f8e600a36f69d5bc3
work_keys_str_mv	AT yanliao upregulationofbrca1associatedringdomain1promoteshepatocellularcarcinomaprogressionbytargetingaktsignaling AT shengguangyuan upregulationofbrca1associatedringdomain1promoteshepatocellularcarcinomaprogressionbytargetingaktsignaling AT xinhuangchen upregulationofbrca1associatedringdomain1promoteshepatocellularcarcinomaprogressionbytargetingaktsignaling AT pengpengzhu upregulationofbrca1associatedringdomain1promoteshepatocellularcarcinomaprogressionbytargetingaktsignaling AT junli upregulationofbrca1associatedringdomain1promoteshepatocellularcarcinomaprogressionbytargetingaktsignaling AT lilingqin upregulationofbrca1associatedringdomain1promoteshepatocellularcarcinomaprogressionbytargetingaktsignaling AT weijialiao upregulationofbrca1associatedringdomain1promoteshepatocellularcarcinomaprogressionbytargetingaktsignaling
_version_	1718384834726854656

Up-regulation of BRCA1-associated RING Domain 1 Promotes Hepatocellular Carcinoma Progression by Targeting Akt Signaling

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