The new psychoactive substances 25H-NBOMe and 25H-NBOH induce abnormal development in the zebrafish embryo and interact in the DNA major groove

Toxicological effects of 25H-NBOMe and 25H-NBOH recreational drugs on zebrafish embryos and larvae at the end of 96 h exposure period were demonstrated. 25H-NBOH and 25H-NBOMe caused high embryo mortality at 80 and 100 µg mL−1, respectively. According to the decrease in the concentration tested, let...

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Autores principales:	Wellington Alves de Barros, Camila da Silva Nunes, Juliana Alves da Costa Ribeiro Souza, Igor José dos Santos Nascimento, Isis Martins Figueiredo, Thiago Mendonça de Aquino, Leonardo Vieira, Davi Farias, Josué Carinhanha Caldas Santos, Ângelo de Fátima
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Phenethylamines Recreational drugs DNA interaction Malformation Embryo mortality Toxicology. Poisons RA1190-1270
Acceso en línea:	https://doaj.org/article/33d8723e2d5e4a208fac2691c0f4621d
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spelling	oai:doaj.org-article:33d8723e2d5e4a208fac2691c0f4621d2021-11-30T04:17:39ZThe new psychoactive substances 25H-NBOMe and 25H-NBOH induce abnormal development in the zebrafish embryo and interact in the DNA major groove2666-027X10.1016/j.crtox.2021.11.002https://doaj.org/article/33d8723e2d5e4a208fac2691c0f4621d2021-01-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2666027X21000402https://doaj.org/toc/2666-027XToxicological effects of 25H-NBOMe and 25H-NBOH recreational drugs on zebrafish embryos and larvae at the end of 96 h exposure period were demonstrated. 25H-NBOH and 25H-NBOMe caused high embryo mortality at 80 and 100 µg mL−1, respectively. According to the decrease in the concentration tested, lethality decreased while non-lethal effects were predominant up to 10 and 50 µg mL−1 of 25H-NBOH and 25H-NBOMe, respectively, including spine malformation, egg hatching delay, body malformation, otolith malformation, pericardial edema, and blood clotting. We can disclose that these drugs have an affinity for DNA in vitro using biophysical spectroscopic assays and molecular modeling methods. The experiments demonstrated that 25H-NBOH and 25H-NBOMe bind to the unclassical major groove of ctDNA with a binding constant of 27.00 × 104 M−1 and 5.27 × 104 M−1, respectively. Furthermore, these interactions lead to conformational changes in the DNA structure. Therefore, the results observed in the zebrafish embryos and DNA may be correlated.Wellington Alves de BarrosCamila da Silva NunesJuliana Alves da Costa Ribeiro SouzaIgor José dos Santos NascimentoIsis Martins FigueiredoThiago Mendonça de AquinoLeonardo VieiraDavi FariasJosué Carinhanha Caldas SantosÂngelo de FátimaElsevierarticlePhenethylaminesRecreational drugsDNA interactionMalformationEmbryo mortalityToxicology. PoisonsRA1190-1270ENCurrent Research in Toxicology, Vol 2, Iss , Pp 386-398 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Phenethylamines Recreational drugs DNA interaction Malformation Embryo mortality Toxicology. Poisons RA1190-1270
spellingShingle	Phenethylamines Recreational drugs DNA interaction Malformation Embryo mortality Toxicology. Poisons RA1190-1270 Wellington Alves de Barros Camila da Silva Nunes Juliana Alves da Costa Ribeiro Souza Igor José dos Santos Nascimento Isis Martins Figueiredo Thiago Mendonça de Aquino Leonardo Vieira Davi Farias Josué Carinhanha Caldas Santos Ângelo de Fátima The new psychoactive substances 25H-NBOMe and 25H-NBOH induce abnormal development in the zebrafish embryo and interact in the DNA major groove
description	Toxicological effects of 25H-NBOMe and 25H-NBOH recreational drugs on zebrafish embryos and larvae at the end of 96 h exposure period were demonstrated. 25H-NBOH and 25H-NBOMe caused high embryo mortality at 80 and 100 µg mL−1, respectively. According to the decrease in the concentration tested, lethality decreased while non-lethal effects were predominant up to 10 and 50 µg mL−1 of 25H-NBOH and 25H-NBOMe, respectively, including spine malformation, egg hatching delay, body malformation, otolith malformation, pericardial edema, and blood clotting. We can disclose that these drugs have an affinity for DNA in vitro using biophysical spectroscopic assays and molecular modeling methods. The experiments demonstrated that 25H-NBOH and 25H-NBOMe bind to the unclassical major groove of ctDNA with a binding constant of 27.00 × 104 M−1 and 5.27 × 104 M−1, respectively. Furthermore, these interactions lead to conformational changes in the DNA structure. Therefore, the results observed in the zebrafish embryos and DNA may be correlated.
format	article
author	Wellington Alves de Barros Camila da Silva Nunes Juliana Alves da Costa Ribeiro Souza Igor José dos Santos Nascimento Isis Martins Figueiredo Thiago Mendonça de Aquino Leonardo Vieira Davi Farias Josué Carinhanha Caldas Santos Ângelo de Fátima
author_facet	Wellington Alves de Barros Camila da Silva Nunes Juliana Alves da Costa Ribeiro Souza Igor José dos Santos Nascimento Isis Martins Figueiredo Thiago Mendonça de Aquino Leonardo Vieira Davi Farias Josué Carinhanha Caldas Santos Ângelo de Fátima
author_sort	Wellington Alves de Barros
title	The new psychoactive substances 25H-NBOMe and 25H-NBOH induce abnormal development in the zebrafish embryo and interact in the DNA major groove
title_short	The new psychoactive substances 25H-NBOMe and 25H-NBOH induce abnormal development in the zebrafish embryo and interact in the DNA major groove
title_full	The new psychoactive substances 25H-NBOMe and 25H-NBOH induce abnormal development in the zebrafish embryo and interact in the DNA major groove
title_fullStr	The new psychoactive substances 25H-NBOMe and 25H-NBOH induce abnormal development in the zebrafish embryo and interact in the DNA major groove
title_full_unstemmed	The new psychoactive substances 25H-NBOMe and 25H-NBOH induce abnormal development in the zebrafish embryo and interact in the DNA major groove
title_sort	new psychoactive substances 25h-nbome and 25h-nboh induce abnormal development in the zebrafish embryo and interact in the dna major groove
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/33d8723e2d5e4a208fac2691c0f4621d
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The new psychoactive substances 25H-NBOMe and 25H-NBOH induce abnormal development in the zebrafish embryo and interact in the DNA major groove

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