Complex-Type N-Glycans Influence the Root Hair Landscape of Arabidopsis Seedlings by Altering the Auxin Output

Roots supply plants with nutrients and water, besides anchoring them in the soil. The primary root with its lateral roots constitutes the central skeleton of the root system. In particular, root hairs increase the root surface, which is critical for optimizing uptake efficiency. During root-cell gro...

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Autores principales:	Manuel Frank, Heidi Kaulfürst-Soboll, Kerstin Fischer, Antje von Schaewen
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	plant hormones N-glycosylation root hairs auxin root development Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/33decb51ed4d438d86624531c4540b43
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spelling	oai:doaj.org-article:33decb51ed4d438d86624531c4540b432021-12-01T20:21:04ZComplex-Type N-Glycans Influence the Root Hair Landscape of Arabidopsis Seedlings by Altering the Auxin Output1664-462X10.3389/fpls.2021.635714https://doaj.org/article/33decb51ed4d438d86624531c4540b432021-02-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fpls.2021.635714/fullhttps://doaj.org/toc/1664-462XRoots supply plants with nutrients and water, besides anchoring them in the soil. The primary root with its lateral roots constitutes the central skeleton of the root system. In particular, root hairs increase the root surface, which is critical for optimizing uptake efficiency. During root-cell growth and development, many proteins that are components of, e.g., the cell wall and plasma membrane are constitutively transported through the secretory system and become posttranslationally modified. Here, the best-studied posttranslational modification is protein N-glycosylation. While alterations in the attachment/modification of N-glycans within the ER lumen results in severe developmental defects, the impact of Golgi-localized complex N-glycan modification, particularly on root development, has not been studied in detail. We report that impairment of complex-type N-glycosylation results in a differential response to synthetic phytohormones with earlier and increased root-hair elongation. Application of either the cytokinin BAP, the auxin NAA, or the ethylene precursor ACC revealed an interaction of auxin with complex N-glycosylation during root-hair development. Especially in gntI mutant seedlings, the early block of complex N-glycan formation resulted in an increased auxin sensitivity. RNA-seq experiments suggest that gntI roots have permanently elevated nutrient-, hypoxia-, and defense-stress responses, which might be a consequence of the altered auxin responsiveness.Manuel FrankManuel FrankHeidi Kaulfürst-SobollKerstin FischerAntje von SchaewenFrontiers Media S.A.articleplant hormonesN-glycosylationroot hairsauxinroot developmentPlant cultureSB1-1110ENFrontiers in Plant Science, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	plant hormones N-glycosylation root hairs auxin root development Plant culture SB1-1110
spellingShingle	plant hormones N-glycosylation root hairs auxin root development Plant culture SB1-1110 Manuel Frank Manuel Frank Heidi Kaulfürst-Soboll Kerstin Fischer Antje von Schaewen Complex-Type N-Glycans Influence the Root Hair Landscape of Arabidopsis Seedlings by Altering the Auxin Output
description	Roots supply plants with nutrients and water, besides anchoring them in the soil. The primary root with its lateral roots constitutes the central skeleton of the root system. In particular, root hairs increase the root surface, which is critical for optimizing uptake efficiency. During root-cell growth and development, many proteins that are components of, e.g., the cell wall and plasma membrane are constitutively transported through the secretory system and become posttranslationally modified. Here, the best-studied posttranslational modification is protein N-glycosylation. While alterations in the attachment/modification of N-glycans within the ER lumen results in severe developmental defects, the impact of Golgi-localized complex N-glycan modification, particularly on root development, has not been studied in detail. We report that impairment of complex-type N-glycosylation results in a differential response to synthetic phytohormones with earlier and increased root-hair elongation. Application of either the cytokinin BAP, the auxin NAA, or the ethylene precursor ACC revealed an interaction of auxin with complex N-glycosylation during root-hair development. Especially in gntI mutant seedlings, the early block of complex N-glycan formation resulted in an increased auxin sensitivity. RNA-seq experiments suggest that gntI roots have permanently elevated nutrient-, hypoxia-, and defense-stress responses, which might be a consequence of the altered auxin responsiveness.
format	article
author	Manuel Frank Manuel Frank Heidi Kaulfürst-Soboll Kerstin Fischer Antje von Schaewen
author_facet	Manuel Frank Manuel Frank Heidi Kaulfürst-Soboll Kerstin Fischer Antje von Schaewen
author_sort	Manuel Frank
title	Complex-Type N-Glycans Influence the Root Hair Landscape of Arabidopsis Seedlings by Altering the Auxin Output
title_short	Complex-Type N-Glycans Influence the Root Hair Landscape of Arabidopsis Seedlings by Altering the Auxin Output
title_full	Complex-Type N-Glycans Influence the Root Hair Landscape of Arabidopsis Seedlings by Altering the Auxin Output
title_fullStr	Complex-Type N-Glycans Influence the Root Hair Landscape of Arabidopsis Seedlings by Altering the Auxin Output
title_full_unstemmed	Complex-Type N-Glycans Influence the Root Hair Landscape of Arabidopsis Seedlings by Altering the Auxin Output
title_sort	complex-type n-glycans influence the root hair landscape of arabidopsis seedlings by altering the auxin output
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/33decb51ed4d438d86624531c4540b43
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Complex-Type N-Glycans Influence the Root Hair Landscape of Arabidopsis Seedlings by Altering the Auxin Output

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