Protective effect and mechanism of dexmedetomidine on lung injury in diabetic mice with myocardial ischemia reperfusion
Objective: To evaluate the protective effect of dexmedetomidine and its mechanism on the lung after myocardial ischemia reperfusion in diabetic mice. Methods: Adult diabetic db/db mice aged 15 weeks were selected. The sham operation group ( S group), ischemic reperfusion group (IR group), dexmede...
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| Autores principales: | , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Editorial Board of Journal of Hainan Medical University
2021
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/33e37e4aa1824249bbe033321416c6c2 |
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| Sumario: | Objective: To evaluate the protective effect of dexmedetomidine and its mechanism on the
lung after myocardial ischemia reperfusion in diabetic mice. Methods: Adult diabetic db/db
mice aged 15 weeks were selected. The sham operation group ( S group), ischemic reperfusion
group (IR group), dexmedetomidine post-treatment group (POST-D) and dexmedetomidine
pre-treatment group (PRE-D) were set respectively. The IR group of mice underwent ligation
of anterior descending branch of left coronary artery for 30min and readministration for
120min.The post-D group and the pre-D group were intraperitoneally injected with 50 ug/
kg DEX before surgery and during reperfusion, respectively.Orbital blood was extracted at
120min of reperfusion,serum levels of creatine kinase isoenzyme MB and inflammatory
cytokines IL-6, IFN-γand IL-10 were detected, then mice were sacrificed and lung tissue
was taken, the ratio of wet and dry weight (W/D) was determined, lung histopathological
morphology was observed under light microscope, superoxide dismutase (SOD)and
malondialdehyde(MDA)contents were determined, Western Blot was used to detect the
expressions of Sirt1, GRP78 and CHOP in lung tissue. Results: Compared with the S group,
the IR group rats had severe lung injury, including increased lung W/D value, increased serum
CK-MB、 IL-6 and IFN-γ levels, decreased serum IL-10, increased serum MDA content,
decreased SOD activity, down-regulated Sirt1 expression, up-regulated GRP78 and CHOP
expression. Compared with IR group, lung histopathological injury was reduced in post-D
group and pre-D group, lung W/D value was decreased, serum CK-MB、IL-6 and IFN-γ
levels were decreased, serum IL-10 was increased, serum MDA content was reduced, SOD
activity was rised, the expression of Sirt1 was up-regulated, and GRP78 and CHOP were
down-regulated. Compared with the post-D group, the degree of lung tissue injury in the pre-D
group was reduced, but the differences in various indicators were not statistically significant.
Conclusion: Dexmedetomidine can alleviate acute lung injury after myocardial ischemia
reperfusion in diabetic mice, and the mechanism may be related to the up-regulation of Sirt1
and the inhibition of endoplasmic reticulum stress |
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