Combined Magnetomotive ultrasound, PET/CT, and MR imaging of 68Ga-labelled superparamagnetic iron oxide nanoparticles in rat sentinel lymph nodes in vivo

Abstract Current methods for intra-surgical guidance to localize metastases at cancer surgery are based on radioactive tracers that cause logistical challenges. We propose the use of a novel ultrasound-based method, magnetomotive ultrasound (MMUS) imaging that employ a nanoparticle-based contrast ag...

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Autores principales:	Maria Evertsson, Pontus Kjellman, Magnus Cinthio, Roger Andersson, Thuy A Tran, Rene in’t Zandt, Gustav Grafström, Hanna Toftevall, Sarah Fredriksson, Christian Ingvar, Sven-Erik Strand, Tomas Jansson
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/33e54c43556c4f1d9a93e51daff32821
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spelling	oai:doaj.org-article:33e54c43556c4f1d9a93e51daff328212021-12-02T16:06:03ZCombined Magnetomotive ultrasound, PET/CT, and MR imaging of 68Ga-labelled superparamagnetic iron oxide nanoparticles in rat sentinel lymph nodes in vivo10.1038/s41598-017-04396-z2045-2322https://doaj.org/article/33e54c43556c4f1d9a93e51daff328212017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04396-zhttps://doaj.org/toc/2045-2322Abstract Current methods for intra-surgical guidance to localize metastases at cancer surgery are based on radioactive tracers that cause logistical challenges. We propose the use of a novel ultrasound-based method, magnetomotive ultrasound (MMUS) imaging that employ a nanoparticle-based contrast agent that also may be used for pre-operative PET/MRI imaging. Since MMUS is radiation free, this eliminates the dependence between pre- and intra-operative imaging and the radiation exposure for the surgical staff. This study investigates a hypothetical clinical scenario of pre-operative PET imaging, combined with intra-operative MMUS imaging, implemented in a sentinel lymph node (SLN) rat model. At one-hour post injection of 68Ga-labelled magnetic nanoparticles, six animals were imaged with combined PET/CT. After two or four days, the same animals were imaged with MMUS. In addition, ex-vivo MRI was used to evaluate the amount of nanoparticles in each single SLN. All SLNs were detectable by PET. Four out of six SLNs could be detected with MMUS, and for these MMUS and MRI measurements were in close agreement. The MRI measurements revealed that the two SLNs undetectable with MMUS contained the lowest nanoparticle concentrations. This study shows that MMUS can complement standard pre-operative imaging by providing bedside real-time images with high spatial resolution.Maria EvertssonPontus KjellmanMagnus CinthioRoger AnderssonThuy A TranRene in’t ZandtGustav GrafströmHanna ToftevallSarah FredrikssonChristian IngvarSven-Erik StrandTomas JanssonNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Maria Evertsson Pontus Kjellman Magnus Cinthio Roger Andersson Thuy A Tran Rene in’t Zandt Gustav Grafström Hanna Toftevall Sarah Fredriksson Christian Ingvar Sven-Erik Strand Tomas Jansson Combined Magnetomotive ultrasound, PET/CT, and MR imaging of 68Ga-labelled superparamagnetic iron oxide nanoparticles in rat sentinel lymph nodes in vivo
description	Abstract Current methods for intra-surgical guidance to localize metastases at cancer surgery are based on radioactive tracers that cause logistical challenges. We propose the use of a novel ultrasound-based method, magnetomotive ultrasound (MMUS) imaging that employ a nanoparticle-based contrast agent that also may be used for pre-operative PET/MRI imaging. Since MMUS is radiation free, this eliminates the dependence between pre- and intra-operative imaging and the radiation exposure for the surgical staff. This study investigates a hypothetical clinical scenario of pre-operative PET imaging, combined with intra-operative MMUS imaging, implemented in a sentinel lymph node (SLN) rat model. At one-hour post injection of 68Ga-labelled magnetic nanoparticles, six animals were imaged with combined PET/CT. After two or four days, the same animals were imaged with MMUS. In addition, ex-vivo MRI was used to evaluate the amount of nanoparticles in each single SLN. All SLNs were detectable by PET. Four out of six SLNs could be detected with MMUS, and for these MMUS and MRI measurements were in close agreement. The MRI measurements revealed that the two SLNs undetectable with MMUS contained the lowest nanoparticle concentrations. This study shows that MMUS can complement standard pre-operative imaging by providing bedside real-time images with high spatial resolution.
format	article
author	Maria Evertsson Pontus Kjellman Magnus Cinthio Roger Andersson Thuy A Tran Rene in’t Zandt Gustav Grafström Hanna Toftevall Sarah Fredriksson Christian Ingvar Sven-Erik Strand Tomas Jansson
author_facet	Maria Evertsson Pontus Kjellman Magnus Cinthio Roger Andersson Thuy A Tran Rene in’t Zandt Gustav Grafström Hanna Toftevall Sarah Fredriksson Christian Ingvar Sven-Erik Strand Tomas Jansson
author_sort	Maria Evertsson
title	Combined Magnetomotive ultrasound, PET/CT, and MR imaging of 68Ga-labelled superparamagnetic iron oxide nanoparticles in rat sentinel lymph nodes in vivo
title_short	Combined Magnetomotive ultrasound, PET/CT, and MR imaging of 68Ga-labelled superparamagnetic iron oxide nanoparticles in rat sentinel lymph nodes in vivo
title_full	Combined Magnetomotive ultrasound, PET/CT, and MR imaging of 68Ga-labelled superparamagnetic iron oxide nanoparticles in rat sentinel lymph nodes in vivo
title_fullStr	Combined Magnetomotive ultrasound, PET/CT, and MR imaging of 68Ga-labelled superparamagnetic iron oxide nanoparticles in rat sentinel lymph nodes in vivo
title_full_unstemmed	Combined Magnetomotive ultrasound, PET/CT, and MR imaging of 68Ga-labelled superparamagnetic iron oxide nanoparticles in rat sentinel lymph nodes in vivo
title_sort	combined magnetomotive ultrasound, pet/ct, and mr imaging of 68ga-labelled superparamagnetic iron oxide nanoparticles in rat sentinel lymph nodes in vivo
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/33e54c43556c4f1d9a93e51daff32821
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Combined Magnetomotive ultrasound, PET/CT, and MR imaging of 68Ga-labelled superparamagnetic iron oxide nanoparticles in rat sentinel lymph nodes in vivo

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