A requirement for FGF signalling in the formation of primitive streak-like intermediates from primitive ectoderm in culture.

<h4>Background</h4>Embryonic stem (ES) cells hold considerable promise as a source of cells with therapeutic potential, including cells that can be used for drug screening and in cell replacement therapies. Differentiation of ES cells into the somatic lineages is a regulated process; bef...

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Autores principales:	Zhiqiang Zheng, Robb U de Iongh, Peter D Rathjen, Joy Rathjen
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/33e9ae7416684234b5e9b7b226f0b774
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spelling	oai:doaj.org-article:33e9ae7416684234b5e9b7b226f0b7742021-11-18T06:35:27ZA requirement for FGF signalling in the formation of primitive streak-like intermediates from primitive ectoderm in culture.1932-620310.1371/journal.pone.0012555https://doaj.org/article/33e9ae7416684234b5e9b7b226f0b7742010-09-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20838439/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Embryonic stem (ES) cells hold considerable promise as a source of cells with therapeutic potential, including cells that can be used for drug screening and in cell replacement therapies. Differentiation of ES cells into the somatic lineages is a regulated process; before the promise of these cells can be realised robust and rational methods for directing differentiation into normal, functional and safe cells need to be developed. Previous in vivo studies have implicated fibroblast growth factor (FGF) signalling in lineage specification from pluripotent cells. Although FGF signalling has been suggested as essential for specification of mesoderm and endoderm in vivo and in culture, the exact role of this pathway remains unclear.<h4>Methodology/principal findings</h4>Using a culture model based on early primitive ectoderm-like (EPL) cells we have investigated the role of FGF signalling in the specification of mesoderm. We were unable to demonstrate any mesoderm inductive capability associated with FGF1, 4 or 8 signalling, even when the factors were present at high concentrations, nor any enhancement in mesoderm formation induced by exogenous BMP4. Furthermore, there was no evidence of alteration of mesoderm sub-type formed with addition of FGF1, 4 or 8. Inhibition of endogenous FGF signalling, however, prevented mesoderm and favoured neural differentiation, suggesting FGF signalling was required but not sufficient for the differentiation of primitive ectoderm into primitive streak-like intermediates. The maintenance of ES cell/early epiblast pluripotent marker expression was also observed in cultures when FGF signalling was inhibited.<h4>Conclusions/significance</h4>FGF signalling has been shown to be required for the differentiation of primitive ectoderm to neurectoderm. This, coupled with our observations, suggest FGF signalling is required for differentiation of the primitive ectoderm into the germ lineages at gastrulation.Zhiqiang ZhengRobb U de IonghPeter D RathjenJoy RathjenPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 9, p e12555 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Zhiqiang Zheng Robb U de Iongh Peter D Rathjen Joy Rathjen A requirement for FGF signalling in the formation of primitive streak-like intermediates from primitive ectoderm in culture.
description	<h4>Background</h4>Embryonic stem (ES) cells hold considerable promise as a source of cells with therapeutic potential, including cells that can be used for drug screening and in cell replacement therapies. Differentiation of ES cells into the somatic lineages is a regulated process; before the promise of these cells can be realised robust and rational methods for directing differentiation into normal, functional and safe cells need to be developed. Previous in vivo studies have implicated fibroblast growth factor (FGF) signalling in lineage specification from pluripotent cells. Although FGF signalling has been suggested as essential for specification of mesoderm and endoderm in vivo and in culture, the exact role of this pathway remains unclear.<h4>Methodology/principal findings</h4>Using a culture model based on early primitive ectoderm-like (EPL) cells we have investigated the role of FGF signalling in the specification of mesoderm. We were unable to demonstrate any mesoderm inductive capability associated with FGF1, 4 or 8 signalling, even when the factors were present at high concentrations, nor any enhancement in mesoderm formation induced by exogenous BMP4. Furthermore, there was no evidence of alteration of mesoderm sub-type formed with addition of FGF1, 4 or 8. Inhibition of endogenous FGF signalling, however, prevented mesoderm and favoured neural differentiation, suggesting FGF signalling was required but not sufficient for the differentiation of primitive ectoderm into primitive streak-like intermediates. The maintenance of ES cell/early epiblast pluripotent marker expression was also observed in cultures when FGF signalling was inhibited.<h4>Conclusions/significance</h4>FGF signalling has been shown to be required for the differentiation of primitive ectoderm to neurectoderm. This, coupled with our observations, suggest FGF signalling is required for differentiation of the primitive ectoderm into the germ lineages at gastrulation.
format	article
author	Zhiqiang Zheng Robb U de Iongh Peter D Rathjen Joy Rathjen
author_facet	Zhiqiang Zheng Robb U de Iongh Peter D Rathjen Joy Rathjen
author_sort	Zhiqiang Zheng
title	A requirement for FGF signalling in the formation of primitive streak-like intermediates from primitive ectoderm in culture.
title_short	A requirement for FGF signalling in the formation of primitive streak-like intermediates from primitive ectoderm in culture.
title_full	A requirement for FGF signalling in the formation of primitive streak-like intermediates from primitive ectoderm in culture.
title_fullStr	A requirement for FGF signalling in the formation of primitive streak-like intermediates from primitive ectoderm in culture.
title_full_unstemmed	A requirement for FGF signalling in the formation of primitive streak-like intermediates from primitive ectoderm in culture.
title_sort	requirement for fgf signalling in the formation of primitive streak-like intermediates from primitive ectoderm in culture.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/33e9ae7416684234b5e9b7b226f0b774
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A requirement for FGF signalling in the formation of primitive streak-like intermediates from primitive ectoderm in culture.

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