Viscosity-adjustable grease matrices for serial nanocrystallography

Abstract Serial femtosecond crystallography (SFX) has enabled determination of room temperature structures of proteins with minimum radiation damage. A highly viscous grease matrix acting as a crystal carrier for serial sample loading at a low flow rate of ~0.5 μl min−1 was introduced into the beam...

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Autores principales:	Michihiro Sugahara, Koji Motomura, Mamoru Suzuki, Tetsuya Masuda, Yasumasa Joti, Keiji Numata, Kensuke Tono, Makina Yabashi, Tetsuya Ishikawa
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3403055837a84cb49e5f3f07b62f9720
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spelling	oai:doaj.org-article:3403055837a84cb49e5f3f07b62f97202021-12-02T14:16:42ZViscosity-adjustable grease matrices for serial nanocrystallography10.1038/s41598-020-57675-72045-2322https://doaj.org/article/3403055837a84cb49e5f3f07b62f97202020-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-57675-7https://doaj.org/toc/2045-2322Abstract Serial femtosecond crystallography (SFX) has enabled determination of room temperature structures of proteins with minimum radiation damage. A highly viscous grease matrix acting as a crystal carrier for serial sample loading at a low flow rate of ~0.5 μl min−1 was introduced into the beam path of X-ray free-electron laser. This matrix makes it possible to determine the protein structure with a sample consumption of less than 1 mg of the protein. The viscosity of the matrix is an important factor in maintaining a continuous and stable sample column from a nozzle of a high viscosity micro-extrusion injector for serial sample loading. Using conventional commercial grease (an oil-based, viscous agent) with insufficient control of viscosity in a matrix often gives an unexpectedly low viscosity, providing an unstable sample stream, with effects such as curling of the stream. Adjustment of the grease viscosity is extremely difficult since the commercial grease contains unknown compounds, which may act as unexpected inhibitors of proteins. This study introduces two novel grease matrix carriers comprising known compounds with a viscosity higher than that of conventional greases, to determine the proteinase K structure from nano-/microcrystals.Michihiro SugaharaKoji MotomuraMamoru SuzukiTetsuya MasudaYasumasa JotiKeiji NumataKensuke TonoMakina YabashiTetsuya IshikawaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-11 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Michihiro Sugahara Koji Motomura Mamoru Suzuki Tetsuya Masuda Yasumasa Joti Keiji Numata Kensuke Tono Makina Yabashi Tetsuya Ishikawa Viscosity-adjustable grease matrices for serial nanocrystallography
description	Abstract Serial femtosecond crystallography (SFX) has enabled determination of room temperature structures of proteins with minimum radiation damage. A highly viscous grease matrix acting as a crystal carrier for serial sample loading at a low flow rate of ~0.5 μl min−1 was introduced into the beam path of X-ray free-electron laser. This matrix makes it possible to determine the protein structure with a sample consumption of less than 1 mg of the protein. The viscosity of the matrix is an important factor in maintaining a continuous and stable sample column from a nozzle of a high viscosity micro-extrusion injector for serial sample loading. Using conventional commercial grease (an oil-based, viscous agent) with insufficient control of viscosity in a matrix often gives an unexpectedly low viscosity, providing an unstable sample stream, with effects such as curling of the stream. Adjustment of the grease viscosity is extremely difficult since the commercial grease contains unknown compounds, which may act as unexpected inhibitors of proteins. This study introduces two novel grease matrix carriers comprising known compounds with a viscosity higher than that of conventional greases, to determine the proteinase K structure from nano-/microcrystals.
format	article
author	Michihiro Sugahara Koji Motomura Mamoru Suzuki Tetsuya Masuda Yasumasa Joti Keiji Numata Kensuke Tono Makina Yabashi Tetsuya Ishikawa
author_facet	Michihiro Sugahara Koji Motomura Mamoru Suzuki Tetsuya Masuda Yasumasa Joti Keiji Numata Kensuke Tono Makina Yabashi Tetsuya Ishikawa
author_sort	Michihiro Sugahara
title	Viscosity-adjustable grease matrices for serial nanocrystallography
title_short	Viscosity-adjustable grease matrices for serial nanocrystallography
title_full	Viscosity-adjustable grease matrices for serial nanocrystallography
title_fullStr	Viscosity-adjustable grease matrices for serial nanocrystallography
title_full_unstemmed	Viscosity-adjustable grease matrices for serial nanocrystallography
title_sort	viscosity-adjustable grease matrices for serial nanocrystallography
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/3403055837a84cb49e5f3f07b62f9720
work_keys_str_mv	AT michihirosugahara viscosityadjustablegreasematricesforserialnanocrystallography AT kojimotomura viscosityadjustablegreasematricesforserialnanocrystallography AT mamorusuzuki viscosityadjustablegreasematricesforserialnanocrystallography AT tetsuyamasuda viscosityadjustablegreasematricesforserialnanocrystallography AT yasumasajoti viscosityadjustablegreasematricesforserialnanocrystallography AT keijinumata viscosityadjustablegreasematricesforserialnanocrystallography AT kensuketono viscosityadjustablegreasematricesforserialnanocrystallography AT makinayabashi viscosityadjustablegreasematricesforserialnanocrystallography AT tetsuyaishikawa viscosityadjustablegreasematricesforserialnanocrystallography
_version_	1718391658265968640

Viscosity-adjustable grease matrices for serial nanocrystallography

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