The Cannabinoid Receptor Interacting Proteins 1 of zebrafish are not required for morphological development, viability or fertility

Abstract The Cannabinoid Receptor Interacting Protein 1 (Cnrip1) was discovered as an interactor with the intracellular region of Cannabinoid Receptor 1 (CB1R, also known as Cnr1 or CB1). Functional assays in mouse show cannabinoid sensitivity changes and Cnrip1 has recently been suggested to contro...

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Autores principales:	Laura Fin, Giorgia Bergamin, Roberto A. Steiner, Simon M. Hughes
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/340f674628fa4d7b958bba46b011b214
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spelling	oai:doaj.org-article:340f674628fa4d7b958bba46b011b2142021-12-02T16:06:50ZThe Cannabinoid Receptor Interacting Proteins 1 of zebrafish are not required for morphological development, viability or fertility10.1038/s41598-017-05017-52045-2322https://doaj.org/article/340f674628fa4d7b958bba46b011b2142017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05017-5https://doaj.org/toc/2045-2322Abstract The Cannabinoid Receptor Interacting Protein 1 (Cnrip1) was discovered as an interactor with the intracellular region of Cannabinoid Receptor 1 (CB1R, also known as Cnr1 or CB1). Functional assays in mouse show cannabinoid sensitivity changes and Cnrip1 has recently been suggested to control eye development in Xenopus laevis. Two Cnrip1 genes are described in zebrafish, cnrip1a and cnrip1b. In situ mRNA hybridisation revealed accumulation of mRNA encoding each gene primarily in brain and spinal cord, but also elsewhere. For example, cnrip1b is expressed in forming skeletal muscle. CRISPR/Cas9 genome editing generated predicted null mutations in cnrip1a and cnrip1b. Each mutation triggered nonsense-mediated decay of the respective mRNA transcript. No morphological or behavioural phenotype was observed in either mutant. Moreover, fish lacking both Cnrip1a and Cnrip1b both maternally and zygotically are viable and fertile and no phenotype has so far been detected despite strong evolutionary conservation over at least 400 Myr.Laura FinGiorgia BergaminRoberto A. SteinerSimon M. HughesNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Laura Fin Giorgia Bergamin Roberto A. Steiner Simon M. Hughes The Cannabinoid Receptor Interacting Proteins 1 of zebrafish are not required for morphological development, viability or fertility
description	Abstract The Cannabinoid Receptor Interacting Protein 1 (Cnrip1) was discovered as an interactor with the intracellular region of Cannabinoid Receptor 1 (CB1R, also known as Cnr1 or CB1). Functional assays in mouse show cannabinoid sensitivity changes and Cnrip1 has recently been suggested to control eye development in Xenopus laevis. Two Cnrip1 genes are described in zebrafish, cnrip1a and cnrip1b. In situ mRNA hybridisation revealed accumulation of mRNA encoding each gene primarily in brain and spinal cord, but also elsewhere. For example, cnrip1b is expressed in forming skeletal muscle. CRISPR/Cas9 genome editing generated predicted null mutations in cnrip1a and cnrip1b. Each mutation triggered nonsense-mediated decay of the respective mRNA transcript. No morphological or behavioural phenotype was observed in either mutant. Moreover, fish lacking both Cnrip1a and Cnrip1b both maternally and zygotically are viable and fertile and no phenotype has so far been detected despite strong evolutionary conservation over at least 400 Myr.
format	article
author	Laura Fin Giorgia Bergamin Roberto A. Steiner Simon M. Hughes
author_facet	Laura Fin Giorgia Bergamin Roberto A. Steiner Simon M. Hughes
author_sort	Laura Fin
title	The Cannabinoid Receptor Interacting Proteins 1 of zebrafish are not required for morphological development, viability or fertility
title_short	The Cannabinoid Receptor Interacting Proteins 1 of zebrafish are not required for morphological development, viability or fertility
title_full	The Cannabinoid Receptor Interacting Proteins 1 of zebrafish are not required for morphological development, viability or fertility
title_fullStr	The Cannabinoid Receptor Interacting Proteins 1 of zebrafish are not required for morphological development, viability or fertility
title_full_unstemmed	The Cannabinoid Receptor Interacting Proteins 1 of zebrafish are not required for morphological development, viability or fertility
title_sort	cannabinoid receptor interacting proteins 1 of zebrafish are not required for morphological development, viability or fertility
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/340f674628fa4d7b958bba46b011b214
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The Cannabinoid Receptor Interacting Proteins 1 of zebrafish are not required for morphological development, viability or fertility

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