Sites of blood collection and topical antiseptics associated with contaminated cultures: prospective observational study

Abstract We aimed to determine whether puncture sites for blood sampling and topical disinfectants are associated with rates of contaminated blood cultures in the emergency department (ED) of a single institution. This single-center, prospective observational study of 249 consecutive patients aged ≥...

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Autores principales:	Koshi Ota, Koji Oba, Keisuke Fukui, Yuri Ito, Emi Hamada, Naomi Mori, Masahiro Oka, Kanna Ota, Yuriko Shibata, Akira Takasu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/341207dc27514e4990611ae9bf62149f
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spelling	oai:doaj.org-article:341207dc27514e4990611ae9bf62149f2021-12-02T13:17:56ZSites of blood collection and topical antiseptics associated with contaminated cultures: prospective observational study10.1038/s41598-021-85614-72045-2322https://doaj.org/article/341207dc27514e4990611ae9bf62149f2021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-85614-7https://doaj.org/toc/2045-2322Abstract We aimed to determine whether puncture sites for blood sampling and topical disinfectants are associated with rates of contaminated blood cultures in the emergency department (ED) of a single institution. This single-center, prospective observational study of 249 consecutive patients aged ≥ 20 years proceeded in the ED of a university hospital in Japan during 6 months. Pairs of blood samples were collected for aerobic and anaerobic culture from all patients in the ED. Physicians selected puncture sites and topical disinfectants according to their personal preference. We found 50 (20.1%) patients with potentially contaminated blood cultures. Fifty-six (22.5%) patients were true bacteremia and 143 (57.4%) patients were true negatives. Multivariate analysis associated more frequent contamination when puncture sites were disinfected with povidone-iodine than with alcohol/chlorhexidine (adjusted risk difference, 12.9%; 95% confidence interval [CI] 8.8–16.9; P < 0.001). Sites of blood collection were also associated with contamination. Femoral and central venous with other sites were associated with contamination more frequently than venous sites (adjusted risk difference), 13.1% (95% CI 8.2–17.9; P < 0.001]) vs. 17.3% (95% CI 3.6–31.0; P = 0.013). Rates of contaminated blood cultures were significantly higher when blood was collected from femoral sites and when povidone-iodine was the topical antiseptic.Koshi OtaKoji ObaKeisuke FukuiYuri ItoEmi HamadaNaomi MoriMasahiro OkaKanna OtaYuriko ShibataAkira TakasuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-6 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Koshi Ota Koji Oba Keisuke Fukui Yuri Ito Emi Hamada Naomi Mori Masahiro Oka Kanna Ota Yuriko Shibata Akira Takasu Sites of blood collection and topical antiseptics associated with contaminated cultures: prospective observational study
description	Abstract We aimed to determine whether puncture sites for blood sampling and topical disinfectants are associated with rates of contaminated blood cultures in the emergency department (ED) of a single institution. This single-center, prospective observational study of 249 consecutive patients aged ≥ 20 years proceeded in the ED of a university hospital in Japan during 6 months. Pairs of blood samples were collected for aerobic and anaerobic culture from all patients in the ED. Physicians selected puncture sites and topical disinfectants according to their personal preference. We found 50 (20.1%) patients with potentially contaminated blood cultures. Fifty-six (22.5%) patients were true bacteremia and 143 (57.4%) patients were true negatives. Multivariate analysis associated more frequent contamination when puncture sites were disinfected with povidone-iodine than with alcohol/chlorhexidine (adjusted risk difference, 12.9%; 95% confidence interval [CI] 8.8–16.9; P < 0.001). Sites of blood collection were also associated with contamination. Femoral and central venous with other sites were associated with contamination more frequently than venous sites (adjusted risk difference), 13.1% (95% CI 8.2–17.9; P < 0.001]) vs. 17.3% (95% CI 3.6–31.0; P = 0.013). Rates of contaminated blood cultures were significantly higher when blood was collected from femoral sites and when povidone-iodine was the topical antiseptic.
format	article
author	Koshi Ota Koji Oba Keisuke Fukui Yuri Ito Emi Hamada Naomi Mori Masahiro Oka Kanna Ota Yuriko Shibata Akira Takasu
author_facet	Koshi Ota Koji Oba Keisuke Fukui Yuri Ito Emi Hamada Naomi Mori Masahiro Oka Kanna Ota Yuriko Shibata Akira Takasu
author_sort	Koshi Ota
title	Sites of blood collection and topical antiseptics associated with contaminated cultures: prospective observational study
title_short	Sites of blood collection and topical antiseptics associated with contaminated cultures: prospective observational study
title_full	Sites of blood collection and topical antiseptics associated with contaminated cultures: prospective observational study
title_fullStr	Sites of blood collection and topical antiseptics associated with contaminated cultures: prospective observational study
title_full_unstemmed	Sites of blood collection and topical antiseptics associated with contaminated cultures: prospective observational study
title_sort	sites of blood collection and topical antiseptics associated with contaminated cultures: prospective observational study
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/341207dc27514e4990611ae9bf62149f
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Sites of blood collection and topical antiseptics associated with contaminated cultures: prospective observational study

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