Alterations in cellular and organellar phospholipid compositions of HepG2 cells during cell growth

Abstract The human hepatoblastoma cell line, HepG2, has been used for investigating a wide variety of physiological and pathophysiological processes. However, less information is available about the phospholipid metabolism in HepG2 cells. In the present report, to clarify the relationship between ce...

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Autores principales:	Tokuji Tsuji, Shin-ya Morita, Yoshinobu Nakamura, Yoshito Ikeda, Taiho Kambe, Tomohiro Terada
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3417288326e148c886f1ec89bf80392d
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spelling	oai:doaj.org-article:3417288326e148c886f1ec89bf80392d2021-12-02T10:44:14ZAlterations in cellular and organellar phospholipid compositions of HepG2 cells during cell growth10.1038/s41598-021-81733-32045-2322https://doaj.org/article/3417288326e148c886f1ec89bf80392d2021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-81733-3https://doaj.org/toc/2045-2322Abstract The human hepatoblastoma cell line, HepG2, has been used for investigating a wide variety of physiological and pathophysiological processes. However, less information is available about the phospholipid metabolism in HepG2 cells. In the present report, to clarify the relationship between cell growth and phospholipid metabolism in HepG2 cells, we examined the phospholipid class compositions of the cells and their intracellular organelles by using enzymatic fluorometric methods. In HepG2 cells, the ratios of all phospholipid classes, but not the ratio of cholesterol, markedly changed with cell growth. Of note, depending on cell growth, the phosphatidic acid (PA) ratio increased and phosphatidylcholine (PC) ratio decreased in the nuclear membranes, the sphingomyelin (SM) ratio increased in the microsomal membranes, and the phosphatidylethanolamine (PE) ratio increased and the phosphatidylserine (PS) ratio decreased in the mitochondrial membranes. Moreover, the mRNA expression levels of enzymes related to PC, PE, PS, PA, SM and cardiolipin syntheses changed during cell growth. We suggest that the phospholipid class compositions of organellar membranes are tightly regulated by cell growth. These findings provide a basis for future investigations of cancer cell growth and lipid metabolism.Tokuji TsujiShin-ya MoritaYoshinobu NakamuraYoshito IkedaTaiho KambeTomohiro TeradaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tokuji Tsuji Shin-ya Morita Yoshinobu Nakamura Yoshito Ikeda Taiho Kambe Tomohiro Terada Alterations in cellular and organellar phospholipid compositions of HepG2 cells during cell growth
description	Abstract The human hepatoblastoma cell line, HepG2, has been used for investigating a wide variety of physiological and pathophysiological processes. However, less information is available about the phospholipid metabolism in HepG2 cells. In the present report, to clarify the relationship between cell growth and phospholipid metabolism in HepG2 cells, we examined the phospholipid class compositions of the cells and their intracellular organelles by using enzymatic fluorometric methods. In HepG2 cells, the ratios of all phospholipid classes, but not the ratio of cholesterol, markedly changed with cell growth. Of note, depending on cell growth, the phosphatidic acid (PA) ratio increased and phosphatidylcholine (PC) ratio decreased in the nuclear membranes, the sphingomyelin (SM) ratio increased in the microsomal membranes, and the phosphatidylethanolamine (PE) ratio increased and the phosphatidylserine (PS) ratio decreased in the mitochondrial membranes. Moreover, the mRNA expression levels of enzymes related to PC, PE, PS, PA, SM and cardiolipin syntheses changed during cell growth. We suggest that the phospholipid class compositions of organellar membranes are tightly regulated by cell growth. These findings provide a basis for future investigations of cancer cell growth and lipid metabolism.
format	article
author	Tokuji Tsuji Shin-ya Morita Yoshinobu Nakamura Yoshito Ikeda Taiho Kambe Tomohiro Terada
author_facet	Tokuji Tsuji Shin-ya Morita Yoshinobu Nakamura Yoshito Ikeda Taiho Kambe Tomohiro Terada
author_sort	Tokuji Tsuji
title	Alterations in cellular and organellar phospholipid compositions of HepG2 cells during cell growth
title_short	Alterations in cellular and organellar phospholipid compositions of HepG2 cells during cell growth
title_full	Alterations in cellular and organellar phospholipid compositions of HepG2 cells during cell growth
title_fullStr	Alterations in cellular and organellar phospholipid compositions of HepG2 cells during cell growth
title_full_unstemmed	Alterations in cellular and organellar phospholipid compositions of HepG2 cells during cell growth
title_sort	alterations in cellular and organellar phospholipid compositions of hepg2 cells during cell growth
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/3417288326e148c886f1ec89bf80392d
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_version_	1718396757080014848

Alterations in cellular and organellar phospholipid compositions of HepG2 cells during cell growth

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