HPV genotyping and p16/Ki-67 test significantly improve detection rate of high-grade cervical squamous intraepithelial lesion

Introduction Liquid-based cytology allows to apply modern and specific analyses of hrHPV genotyping in p16/Ki-67 test. All of these together could raise accuracy ratio for high-grade squamous intraepithelial lesion above 90%. The purpose of this study was to evaluate the diagnostic accuracy of LBC,...

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Autores principales:	Piotr Lewitowicz, Anna Nasierowska-Guttmejer, Wojciech Rokita, Olga Adamczyk-Gruszka, Stanisław Gluszek, Magdalena Chrapek, Małgorzata Kolos, Agnieszka Wrona-Cyranowska, Marcin Misiek
Formato:	article
Lenguaje:	EN
Publicado:	Termedia Publishing House 2019
Materias:	cervical cancer cancer screening ki67/p16 liquid base cytology human papilomavirus Medicine R
Acceso en línea:	https://doaj.org/article/3420c44112d44f11bacaa265d1405e4d
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spelling	oai:doaj.org-article:3420c44112d44f11bacaa265d1405e4d2021-12-02T18:33:05ZHPV genotyping and p16/Ki-67 test significantly improve detection rate of high-grade cervical squamous intraepithelial lesion1734-19221896-915110.5114/aoms.2018.80697https://doaj.org/article/3420c44112d44f11bacaa265d1405e4d2019-12-01T00:00:00Zhttps://www.archivesofmedicalscience.com/HPV-genotyping-and-p16-Ki-67-test-significantly-improve-detection-rate-of-high-grade,99678,0,2.htmlhttps://doaj.org/toc/1734-1922https://doaj.org/toc/1896-9151Introduction Liquid-based cytology allows to apply modern and specific analyses of hrHPV genotyping in p16/Ki-67 test. All of these together could raise accuracy ratio for high-grade squamous intraepithelial lesion above 90%. The purpose of this study was to evaluate the diagnostic accuracy of LBC, hrHPV testing, and p16/Ki-67 testing in diagnosis of high-grade cervical intraepithelial lesions. Material and methods The study consisted of 176 women, out of which 50 presented with HSIL (CIN2) SCC (cervical intraepithelial lesion grade 2 squamous cell carcinoma). 126 women with a negative Pap test were pooled into the second group of the study. All patients were resampled for LBC, HPV genotyping, and for the p16/Ki-67 test. The research was carried out between May and December 2017, and second sampling were taken from 1 to 4 months. Results We reported a strong correlation between positive Pap test and hrHPV (p < 0.05) that met accuracy close to 90%. We noted correlations between a positive p16/Ki-67 with a positive Pap test: p < 0.001; 66% sensitivity (95% CI: 51.2–78.8%), 87.8% specificity (95% CI: 75.2–95.4%), 76.8% accuracy (95% CI: 67.2–84.7%), and OR 13.9 (95% CI: 4.9–39.2), especially HSIL and HPV16: p < 0.001; sensitivity (95% CI) 64.0, specificity (95% CI) 98.4, accuracy (95% CI) 88.6, OR (95% CI) 109.3. Conclusions The results of our study indicate hrHPV genotyping as a good biomarker for the triage of patients with an abnormal cytological report. In our opinion, the hrHPV test reaches the highest level of sensitivity, specificity, and accuracy, and should be considered as crucial diagnostic test in cervical screening.Piotr LewitowiczAnna Nasierowska-GuttmejerWojciech RokitaOlga Adamczyk-GruszkaStanisław GluszekMagdalena ChrapekMałgorzata KolosAgnieszka Wrona-CyranowskaMarcin MisiekTermedia Publishing Housearticlecervical cancercancer screeningki67/p16liquid base cytologyhuman papilomavirusMedicineRENArchives of Medical Science, Vol 16, Iss 1, Pp 87-93 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	cervical cancer cancer screening ki67/p16 liquid base cytology human papilomavirus Medicine R
spellingShingle	cervical cancer cancer screening ki67/p16 liquid base cytology human papilomavirus Medicine R Piotr Lewitowicz Anna Nasierowska-Guttmejer Wojciech Rokita Olga Adamczyk-Gruszka Stanisław Gluszek Magdalena Chrapek Małgorzata Kolos Agnieszka Wrona-Cyranowska Marcin Misiek HPV genotyping and p16/Ki-67 test significantly improve detection rate of high-grade cervical squamous intraepithelial lesion
description	Introduction Liquid-based cytology allows to apply modern and specific analyses of hrHPV genotyping in p16/Ki-67 test. All of these together could raise accuracy ratio for high-grade squamous intraepithelial lesion above 90%. The purpose of this study was to evaluate the diagnostic accuracy of LBC, hrHPV testing, and p16/Ki-67 testing in diagnosis of high-grade cervical intraepithelial lesions. Material and methods The study consisted of 176 women, out of which 50 presented with HSIL (CIN2) SCC (cervical intraepithelial lesion grade 2 squamous cell carcinoma). 126 women with a negative Pap test were pooled into the second group of the study. All patients were resampled for LBC, HPV genotyping, and for the p16/Ki-67 test. The research was carried out between May and December 2017, and second sampling were taken from 1 to 4 months. Results We reported a strong correlation between positive Pap test and hrHPV (p < 0.05) that met accuracy close to 90%. We noted correlations between a positive p16/Ki-67 with a positive Pap test: p < 0.001; 66% sensitivity (95% CI: 51.2–78.8%), 87.8% specificity (95% CI: 75.2–95.4%), 76.8% accuracy (95% CI: 67.2–84.7%), and OR 13.9 (95% CI: 4.9–39.2), especially HSIL and HPV16: p < 0.001; sensitivity (95% CI) 64.0, specificity (95% CI) 98.4, accuracy (95% CI) 88.6, OR (95% CI) 109.3. Conclusions The results of our study indicate hrHPV genotyping as a good biomarker for the triage of patients with an abnormal cytological report. In our opinion, the hrHPV test reaches the highest level of sensitivity, specificity, and accuracy, and should be considered as crucial diagnostic test in cervical screening.
format	article
author	Piotr Lewitowicz Anna Nasierowska-Guttmejer Wojciech Rokita Olga Adamczyk-Gruszka Stanisław Gluszek Magdalena Chrapek Małgorzata Kolos Agnieszka Wrona-Cyranowska Marcin Misiek
author_facet	Piotr Lewitowicz Anna Nasierowska-Guttmejer Wojciech Rokita Olga Adamczyk-Gruszka Stanisław Gluszek Magdalena Chrapek Małgorzata Kolos Agnieszka Wrona-Cyranowska Marcin Misiek
author_sort	Piotr Lewitowicz
title	HPV genotyping and p16/Ki-67 test significantly improve detection rate of high-grade cervical squamous intraepithelial lesion
title_short	HPV genotyping and p16/Ki-67 test significantly improve detection rate of high-grade cervical squamous intraepithelial lesion
title_full	HPV genotyping and p16/Ki-67 test significantly improve detection rate of high-grade cervical squamous intraepithelial lesion
title_fullStr	HPV genotyping and p16/Ki-67 test significantly improve detection rate of high-grade cervical squamous intraepithelial lesion
title_full_unstemmed	HPV genotyping and p16/Ki-67 test significantly improve detection rate of high-grade cervical squamous intraepithelial lesion
title_sort	hpv genotyping and p16/ki-67 test significantly improve detection rate of high-grade cervical squamous intraepithelial lesion
publisher	Termedia Publishing House
publishDate	2019
url	https://doaj.org/article/3420c44112d44f11bacaa265d1405e4d
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HPV genotyping and p16/Ki-67 test significantly improve detection rate of high-grade cervical squamous intraepithelial lesion

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