Integrated miRNA-/mRNA-Seq of the Habenulo-Interpeduncular Circuit During Acute Nicotine Withdrawal

Abstract Tobacco use is the leading preventable cause of mortality in the world. The limited number of smoking cessation aids currently available are minimally effective, highlighting the need for novel therapeutic interventions. We describe a genome-wide approach to identify potential candidates fo...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Alison P. Casserly, Junko Tsuji, Rubing Zhao-Shea, Ciearra B. Smith, Susanna Molas, Andrew R. Tapper, Zhiping Weng, Paul D. Gardner
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/34279bfdabda45b49d57ee942945b463
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:34279bfdabda45b49d57ee942945b463
record_format	dspace
spelling	oai:doaj.org-article:34279bfdabda45b49d57ee942945b4632021-12-02T15:23:48ZIntegrated miRNA-/mRNA-Seq of the Habenulo-Interpeduncular Circuit During Acute Nicotine Withdrawal10.1038/s41598-020-57907-w2045-2322https://doaj.org/article/34279bfdabda45b49d57ee942945b4632020-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-57907-whttps://doaj.org/toc/2045-2322Abstract Tobacco use is the leading preventable cause of mortality in the world. The limited number of smoking cessation aids currently available are minimally effective, highlighting the need for novel therapeutic interventions. We describe a genome-wide approach to identify potential candidates for such interventions. Next-generation sequencing was performed using RNA isolated from the habenulo-interpeduncular circuit of male mice withdrawn from chronic nicotine treatment. This circuit plays a central role in the nicotine withdrawal response. Differentially expressed miRNAs and mRNAs were validated using RT-qPCR. Many of the differentially expressed mRNAs are predicted targets of reciprocally expressed miRNAs. We illustrate the utility of the dataset by demonstrating that knockdown in the interpeduncular nucleus of a differentially expressed mRNA, that encoding profilin 2, is sufficient to induce anxiety-related behavior. Importantly, profilin 2 knockdown in the ventral tegmental area did not affect anxiety behavior. Our data reveal wide-spread changes in gene expression within the habenulo-interpeduncular circuit during nicotine withdrawal. This dataset should prove to be a valuable resource leading to the identification of substrates for the design of innovative smoking cessation aids.Alison P. CasserlyJunko TsujiRubing Zhao-SheaCiearra B. SmithSusanna MolasAndrew R. TapperZhiping WengPaul D. GardnerNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-14 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Alison P. Casserly Junko Tsuji Rubing Zhao-Shea Ciearra B. Smith Susanna Molas Andrew R. Tapper Zhiping Weng Paul D. Gardner Integrated miRNA-/mRNA-Seq of the Habenulo-Interpeduncular Circuit During Acute Nicotine Withdrawal
description	Abstract Tobacco use is the leading preventable cause of mortality in the world. The limited number of smoking cessation aids currently available are minimally effective, highlighting the need for novel therapeutic interventions. We describe a genome-wide approach to identify potential candidates for such interventions. Next-generation sequencing was performed using RNA isolated from the habenulo-interpeduncular circuit of male mice withdrawn from chronic nicotine treatment. This circuit plays a central role in the nicotine withdrawal response. Differentially expressed miRNAs and mRNAs were validated using RT-qPCR. Many of the differentially expressed mRNAs are predicted targets of reciprocally expressed miRNAs. We illustrate the utility of the dataset by demonstrating that knockdown in the interpeduncular nucleus of a differentially expressed mRNA, that encoding profilin 2, is sufficient to induce anxiety-related behavior. Importantly, profilin 2 knockdown in the ventral tegmental area did not affect anxiety behavior. Our data reveal wide-spread changes in gene expression within the habenulo-interpeduncular circuit during nicotine withdrawal. This dataset should prove to be a valuable resource leading to the identification of substrates for the design of innovative smoking cessation aids.
format	article
author	Alison P. Casserly Junko Tsuji Rubing Zhao-Shea Ciearra B. Smith Susanna Molas Andrew R. Tapper Zhiping Weng Paul D. Gardner
author_facet	Alison P. Casserly Junko Tsuji Rubing Zhao-Shea Ciearra B. Smith Susanna Molas Andrew R. Tapper Zhiping Weng Paul D. Gardner
author_sort	Alison P. Casserly
title	Integrated miRNA-/mRNA-Seq of the Habenulo-Interpeduncular Circuit During Acute Nicotine Withdrawal
title_short	Integrated miRNA-/mRNA-Seq of the Habenulo-Interpeduncular Circuit During Acute Nicotine Withdrawal
title_full	Integrated miRNA-/mRNA-Seq of the Habenulo-Interpeduncular Circuit During Acute Nicotine Withdrawal
title_fullStr	Integrated miRNA-/mRNA-Seq of the Habenulo-Interpeduncular Circuit During Acute Nicotine Withdrawal
title_full_unstemmed	Integrated miRNA-/mRNA-Seq of the Habenulo-Interpeduncular Circuit During Acute Nicotine Withdrawal
title_sort	integrated mirna-/mrna-seq of the habenulo-interpeduncular circuit during acute nicotine withdrawal
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/34279bfdabda45b49d57ee942945b463
work_keys_str_mv	AT alisonpcasserly integratedmirnamrnaseqofthehabenulointerpeduncularcircuitduringacutenicotinewithdrawal AT junkotsuji integratedmirnamrnaseqofthehabenulointerpeduncularcircuitduringacutenicotinewithdrawal AT rubingzhaoshea integratedmirnamrnaseqofthehabenulointerpeduncularcircuitduringacutenicotinewithdrawal AT ciearrabsmith integratedmirnamrnaseqofthehabenulointerpeduncularcircuitduringacutenicotinewithdrawal AT susannamolas integratedmirnamrnaseqofthehabenulointerpeduncularcircuitduringacutenicotinewithdrawal AT andrewrtapper integratedmirnamrnaseqofthehabenulointerpeduncularcircuitduringacutenicotinewithdrawal AT zhipingweng integratedmirnamrnaseqofthehabenulointerpeduncularcircuitduringacutenicotinewithdrawal AT pauldgardner integratedmirnamrnaseqofthehabenulointerpeduncularcircuitduringacutenicotinewithdrawal
_version_	1718387255247110144

Integrated miRNA-/mRNA-Seq of the Habenulo-Interpeduncular Circuit During Acute Nicotine Withdrawal

Ejemplares similares