Dual-barcoded shotgun expression library sequencing for high-throughput characterization of functional traits in bacteria

Gain of function methods based on gene overexpression are not easily applied to high-throughput screening across different experimental conditions. Here, the authors present Dub-seq, which separates overexpression library characterization from functional screening and uses random DNA barcodes to inc...

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Autores principales:	Vivek K. Mutalik, Pavel S. Novichkov, Morgan N. Price, Trenton K. Owens, Mark Callaghan, Sean Carim, Adam M. Deutschbauer, Adam P. Arkin
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/3444650c24824da0912c4a2da5a186b7
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spelling	oai:doaj.org-article:3444650c24824da0912c4a2da5a186b72021-12-02T16:57:08ZDual-barcoded shotgun expression library sequencing for high-throughput characterization of functional traits in bacteria10.1038/s41467-018-08177-82041-1723https://doaj.org/article/3444650c24824da0912c4a2da5a186b72019-01-01T00:00:00Zhttps://doi.org/10.1038/s41467-018-08177-8https://doaj.org/toc/2041-1723Gain of function methods based on gene overexpression are not easily applied to high-throughput screening across different experimental conditions. Here, the authors present Dub-seq, which separates overexpression library characterization from functional screening and uses random DNA barcodes to increase the experimental throughput.Vivek K. MutalikPavel S. NovichkovMorgan N. PriceTrenton K. OwensMark CallaghanSean CarimAdam M. DeutschbauerAdam P. ArkinNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-14 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Vivek K. Mutalik Pavel S. Novichkov Morgan N. Price Trenton K. Owens Mark Callaghan Sean Carim Adam M. Deutschbauer Adam P. Arkin Dual-barcoded shotgun expression library sequencing for high-throughput characterization of functional traits in bacteria
description	Gain of function methods based on gene overexpression are not easily applied to high-throughput screening across different experimental conditions. Here, the authors present Dub-seq, which separates overexpression library characterization from functional screening and uses random DNA barcodes to increase the experimental throughput.
format	article
author	Vivek K. Mutalik Pavel S. Novichkov Morgan N. Price Trenton K. Owens Mark Callaghan Sean Carim Adam M. Deutschbauer Adam P. Arkin
author_facet	Vivek K. Mutalik Pavel S. Novichkov Morgan N. Price Trenton K. Owens Mark Callaghan Sean Carim Adam M. Deutschbauer Adam P. Arkin
author_sort	Vivek K. Mutalik
title	Dual-barcoded shotgun expression library sequencing for high-throughput characterization of functional traits in bacteria
title_short	Dual-barcoded shotgun expression library sequencing for high-throughput characterization of functional traits in bacteria
title_full	Dual-barcoded shotgun expression library sequencing for high-throughput characterization of functional traits in bacteria
title_fullStr	Dual-barcoded shotgun expression library sequencing for high-throughput characterization of functional traits in bacteria
title_full_unstemmed	Dual-barcoded shotgun expression library sequencing for high-throughput characterization of functional traits in bacteria
title_sort	dual-barcoded shotgun expression library sequencing for high-throughput characterization of functional traits in bacteria
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/3444650c24824da0912c4a2da5a186b7
work_keys_str_mv	AT vivekkmutalik dualbarcodedshotgunexpressionlibrarysequencingforhighthroughputcharacterizationoffunctionaltraitsinbacteria AT pavelsnovichkov dualbarcodedshotgunexpressionlibrarysequencingforhighthroughputcharacterizationoffunctionaltraitsinbacteria AT morgannprice dualbarcodedshotgunexpressionlibrarysequencingforhighthroughputcharacterizationoffunctionaltraitsinbacteria AT trentonkowens dualbarcodedshotgunexpressionlibrarysequencingforhighthroughputcharacterizationoffunctionaltraitsinbacteria AT markcallaghan dualbarcodedshotgunexpressionlibrarysequencingforhighthroughputcharacterizationoffunctionaltraitsinbacteria AT seancarim dualbarcodedshotgunexpressionlibrarysequencingforhighthroughputcharacterizationoffunctionaltraitsinbacteria AT adammdeutschbauer dualbarcodedshotgunexpressionlibrarysequencingforhighthroughputcharacterizationoffunctionaltraitsinbacteria AT adamparkin dualbarcodedshotgunexpressionlibrarysequencingforhighthroughputcharacterizationoffunctionaltraitsinbacteria
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Dual-barcoded shotgun expression library sequencing for high-throughput characterization of functional traits in bacteria

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