MicroRNA miR-30a inhibits cisplatin resistance in ovarian cancer cells through autophagy

In order to study whether microRNA miR-30a inhibits the autophagy through transforming growth factor (TGF)-β/Smad4 to generate cisplatin (DDP) resistance in ovarian cancer cells. The expression of miR-30a, Smad4 and TGF-β was detected in serum of ovarian cancer patients and DDP-resistant cell lines...

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Autores principales:	Yi Cai, Baiping An, Dejiao Yao, Hong Zhou, Jie Zhu
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	mir-30a sensitivity ovarian cancer cisplatin autophagy Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/34546c92f0ec402b8e48098e4fb237f6
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spelling	oai:doaj.org-article:34546c92f0ec402b8e48098e4fb237f62021-11-11T14:23:43ZMicroRNA miR-30a inhibits cisplatin resistance in ovarian cancer cells through autophagy2165-59792165-598710.1080/21655979.2021.2001989https://doaj.org/article/34546c92f0ec402b8e48098e4fb237f62021-11-01T00:00:00Zhttp://dx.doi.org/10.1080/21655979.2021.2001989https://doaj.org/toc/2165-5979https://doaj.org/toc/2165-5987In order to study whether microRNA miR-30a inhibits the autophagy through transforming growth factor (TGF)-β/Smad4 to generate cisplatin (DDP) resistance in ovarian cancer cells. The expression of miR-30a, Smad4 and TGF-β was detected in serum of ovarian cancer patients and DDP-resistant cell lines (A2780) by quantitative real-time polymerase chain reaction (qRT-PCR). Computational search, and western blot were used to demonstrate the downstream target of miR-30a in ovarian cancer cells. Cell viability was measured with CCK8 assay. Apoptosis and autophagy of ovarian cancer cells were analyzed by flow cytometry and transmission electron microscopy, and the expressions of Beclin1 and LC3\II protein were detected by western blot. Expression of miR-30a was significantly decreased while expressions of TGF-β and Smad4 mRNA were increased in serum of ovarian cancer patients after DDP chemotherapy as well as in DDP-resistant cells. Activation of autophagy contributed to DDP-resistance cells. Moreover, Bioinformatics software predicted Smad4 to be a target of miR-30a. Overexpression of miR-30a decreased the expression of Smad4 and TGF-β. Additionally, miR-30a-overexpressing inhibited DDP-induce autophagy and promoted DDP-resistant cells apoptosis. In conclusion, miR-30a mediates DDP resistance in ovarian cancer by inhibiting autophagy via the TGF-β/Smad4 pathway.Yi CaiBaiping AnDejiao YaoHong ZhouJie ZhuTaylor & Francis Grouparticlemir-30asensitivityovarian cancercisplatinautophagyBiotechnologyTP248.13-248.65ENBioengineered, Vol 0, Iss 0 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	mir-30a sensitivity ovarian cancer cisplatin autophagy Biotechnology TP248.13-248.65
spellingShingle	mir-30a sensitivity ovarian cancer cisplatin autophagy Biotechnology TP248.13-248.65 Yi Cai Baiping An Dejiao Yao Hong Zhou Jie Zhu MicroRNA miR-30a inhibits cisplatin resistance in ovarian cancer cells through autophagy
description	In order to study whether microRNA miR-30a inhibits the autophagy through transforming growth factor (TGF)-β/Smad4 to generate cisplatin (DDP) resistance in ovarian cancer cells. The expression of miR-30a, Smad4 and TGF-β was detected in serum of ovarian cancer patients and DDP-resistant cell lines (A2780) by quantitative real-time polymerase chain reaction (qRT-PCR). Computational search, and western blot were used to demonstrate the downstream target of miR-30a in ovarian cancer cells. Cell viability was measured with CCK8 assay. Apoptosis and autophagy of ovarian cancer cells were analyzed by flow cytometry and transmission electron microscopy, and the expressions of Beclin1 and LC3\II protein were detected by western blot. Expression of miR-30a was significantly decreased while expressions of TGF-β and Smad4 mRNA were increased in serum of ovarian cancer patients after DDP chemotherapy as well as in DDP-resistant cells. Activation of autophagy contributed to DDP-resistance cells. Moreover, Bioinformatics software predicted Smad4 to be a target of miR-30a. Overexpression of miR-30a decreased the expression of Smad4 and TGF-β. Additionally, miR-30a-overexpressing inhibited DDP-induce autophagy and promoted DDP-resistant cells apoptosis. In conclusion, miR-30a mediates DDP resistance in ovarian cancer by inhibiting autophagy via the TGF-β/Smad4 pathway.
format	article
author	Yi Cai Baiping An Dejiao Yao Hong Zhou Jie Zhu
author_facet	Yi Cai Baiping An Dejiao Yao Hong Zhou Jie Zhu
author_sort	Yi Cai
title	MicroRNA miR-30a inhibits cisplatin resistance in ovarian cancer cells through autophagy
title_short	MicroRNA miR-30a inhibits cisplatin resistance in ovarian cancer cells through autophagy
title_full	MicroRNA miR-30a inhibits cisplatin resistance in ovarian cancer cells through autophagy
title_fullStr	MicroRNA miR-30a inhibits cisplatin resistance in ovarian cancer cells through autophagy
title_full_unstemmed	MicroRNA miR-30a inhibits cisplatin resistance in ovarian cancer cells through autophagy
title_sort	microrna mir-30a inhibits cisplatin resistance in ovarian cancer cells through autophagy
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/34546c92f0ec402b8e48098e4fb237f6
work_keys_str_mv	AT yicai micrornamir30ainhibitscisplatinresistanceinovariancancercellsthroughautophagy AT baipingan micrornamir30ainhibitscisplatinresistanceinovariancancercellsthroughautophagy AT dejiaoyao micrornamir30ainhibitscisplatinresistanceinovariancancercellsthroughautophagy AT hongzhou micrornamir30ainhibitscisplatinresistanceinovariancancercellsthroughautophagy AT jiezhu micrornamir30ainhibitscisplatinresistanceinovariancancercellsthroughautophagy
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MicroRNA miR-30a inhibits cisplatin resistance in ovarian cancer cells through autophagy

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