GlomSpheres as a 3D co-culture spheroid model of the kidney glomerulus for rapid drug-screening

Tuffin et al. demonstrate a method for culturing conditionally immortalized human podocytes and GEnCs in 3D by forming spheroid cultures termed GlomSpheres, which are found to segregate in an organized manner. These are shown to establish a GBM-like extracellular matrix with increased cell different...

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Autores principales:	Jack Tuffin, Musleeha Chesor, Valeryia Kuzmuk, Tim Johnson, Simon C. Satchell, Gavin I. Welsh, Moin A. Saleem
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/3454bab16428421b8cab931ece01a44c
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spelling	oai:doaj.org-article:3454bab16428421b8cab931ece01a44c2021-12-05T12:08:16ZGlomSpheres as a 3D co-culture spheroid model of the kidney glomerulus for rapid drug-screening10.1038/s42003-021-02868-72399-3642https://doaj.org/article/3454bab16428421b8cab931ece01a44c2021-12-01T00:00:00Zhttps://doi.org/10.1038/s42003-021-02868-7https://doaj.org/toc/2399-3642Tuffin et al. demonstrate a method for culturing conditionally immortalized human podocytes and GEnCs in 3D by forming spheroid cultures termed GlomSpheres, which are found to segregate in an organized manner. These are shown to establish a GBM-like extracellular matrix with increased cell differentiation and maturity when compared to 2D cultures and their use demonstrated in pharmaceutical screening using rapid imaging approaches.Jack TuffinMusleeha ChesorValeryia KuzmukTim JohnsonSimon C. SatchellGavin I. WelshMoin A. SaleemNature PortfolioarticleBiology (General)QH301-705.5ENCommunications Biology, Vol 4, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Jack Tuffin Musleeha Chesor Valeryia Kuzmuk Tim Johnson Simon C. Satchell Gavin I. Welsh Moin A. Saleem GlomSpheres as a 3D co-culture spheroid model of the kidney glomerulus for rapid drug-screening
description	Tuffin et al. demonstrate a method for culturing conditionally immortalized human podocytes and GEnCs in 3D by forming spheroid cultures termed GlomSpheres, which are found to segregate in an organized manner. These are shown to establish a GBM-like extracellular matrix with increased cell differentiation and maturity when compared to 2D cultures and their use demonstrated in pharmaceutical screening using rapid imaging approaches.
format	article
author	Jack Tuffin Musleeha Chesor Valeryia Kuzmuk Tim Johnson Simon C. Satchell Gavin I. Welsh Moin A. Saleem
author_facet	Jack Tuffin Musleeha Chesor Valeryia Kuzmuk Tim Johnson Simon C. Satchell Gavin I. Welsh Moin A. Saleem
author_sort	Jack Tuffin
title	GlomSpheres as a 3D co-culture spheroid model of the kidney glomerulus for rapid drug-screening
title_short	GlomSpheres as a 3D co-culture spheroid model of the kidney glomerulus for rapid drug-screening
title_full	GlomSpheres as a 3D co-culture spheroid model of the kidney glomerulus for rapid drug-screening
title_fullStr	GlomSpheres as a 3D co-culture spheroid model of the kidney glomerulus for rapid drug-screening
title_full_unstemmed	GlomSpheres as a 3D co-culture spheroid model of the kidney glomerulus for rapid drug-screening
title_sort	glomspheres as a 3d co-culture spheroid model of the kidney glomerulus for rapid drug-screening
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/3454bab16428421b8cab931ece01a44c
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GlomSpheres as a 3D co-culture spheroid model of the kidney glomerulus for rapid drug-screening

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