Genotyping Brucella canis isolates using a highly discriminatory multilocus variable-number tandem-repeat analysis (MLVA) assay

Abstract Differentiation of Brucella canis from other Brucella species are mainly performed through PCR-based methods and multilocus variable-number tandem-repeat (VNTR) analysis (MLVA) procedures. Both PCR-based and MLVA methods are limited in discriminating B. canis strains. A new MLVA-13Bc method...

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Autores principales:	Yi Yang, Yin Wang, Elizabeth Poulsen, Russell Ransburgh, Xuming Liu, Baoyan An, Nanyan Lu, Gary Anderson, Chengming Wang, Jianfa Bai
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3461f6765e614c1387ad6e2d2a35190b
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spelling	oai:doaj.org-article:3461f6765e614c1387ad6e2d2a35190b2021-12-02T15:05:28ZGenotyping Brucella canis isolates using a highly discriminatory multilocus variable-number tandem-repeat analysis (MLVA) assay10.1038/s41598-017-01114-72045-2322https://doaj.org/article/3461f6765e614c1387ad6e2d2a35190b2017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01114-7https://doaj.org/toc/2045-2322Abstract Differentiation of Brucella canis from other Brucella species are mainly performed through PCR-based methods and multilocus variable-number tandem-repeat (VNTR) analysis (MLVA) procedures. Both PCR-based and MLVA methods are limited in discriminating B. canis strains. A new MLVA-13Bc method for B. canis genotyping was established by combining eight newly-developed VNTRs with five published ones. During 2010 and 2016, 377 B. canis PCR-positives were identified from 6,844 canine blood samples from 22 U.S. states, resulting in 229 B. canis isolates. The MLVA-13Bc method was able to differentiate each of these 229 isolates. The Hunter-Gaston Discriminatory Index of the individual VNTR loci ranged from 0.516 to 0.934 and the combined discriminatory index reached 1.000. Three major clusters (A, B and C) and 10 genotype groups were identified from the 229 B. canis isolates. Cluster A mainly contains genotype groups 1 and 2, and a few group 3 isolates; nearly all Cluster B isolates were from group 6; other genotype groups were classified into Cluster C. Our newly developed MLVA-13Bc assay is a highly discriminatory assay for B. canis genotyping, and can serve as a useful molecular epidemiological tool, especially for tracing the source of contamination in an event of a B. canis outbreak.Yi YangYin WangElizabeth PoulsenRussell RansburghXuming LiuBaoyan AnNanyan LuGary AndersonChengming WangJianfa BaiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yi Yang Yin Wang Elizabeth Poulsen Russell Ransburgh Xuming Liu Baoyan An Nanyan Lu Gary Anderson Chengming Wang Jianfa Bai Genotyping Brucella canis isolates using a highly discriminatory multilocus variable-number tandem-repeat analysis (MLVA) assay
description	Abstract Differentiation of Brucella canis from other Brucella species are mainly performed through PCR-based methods and multilocus variable-number tandem-repeat (VNTR) analysis (MLVA) procedures. Both PCR-based and MLVA methods are limited in discriminating B. canis strains. A new MLVA-13Bc method for B. canis genotyping was established by combining eight newly-developed VNTRs with five published ones. During 2010 and 2016, 377 B. canis PCR-positives were identified from 6,844 canine blood samples from 22 U.S. states, resulting in 229 B. canis isolates. The MLVA-13Bc method was able to differentiate each of these 229 isolates. The Hunter-Gaston Discriminatory Index of the individual VNTR loci ranged from 0.516 to 0.934 and the combined discriminatory index reached 1.000. Three major clusters (A, B and C) and 10 genotype groups were identified from the 229 B. canis isolates. Cluster A mainly contains genotype groups 1 and 2, and a few group 3 isolates; nearly all Cluster B isolates were from group 6; other genotype groups were classified into Cluster C. Our newly developed MLVA-13Bc assay is a highly discriminatory assay for B. canis genotyping, and can serve as a useful molecular epidemiological tool, especially for tracing the source of contamination in an event of a B. canis outbreak.
format	article
author	Yi Yang Yin Wang Elizabeth Poulsen Russell Ransburgh Xuming Liu Baoyan An Nanyan Lu Gary Anderson Chengming Wang Jianfa Bai
author_facet	Yi Yang Yin Wang Elizabeth Poulsen Russell Ransburgh Xuming Liu Baoyan An Nanyan Lu Gary Anderson Chengming Wang Jianfa Bai
author_sort	Yi Yang
title	Genotyping Brucella canis isolates using a highly discriminatory multilocus variable-number tandem-repeat analysis (MLVA) assay
title_short	Genotyping Brucella canis isolates using a highly discriminatory multilocus variable-number tandem-repeat analysis (MLVA) assay
title_full	Genotyping Brucella canis isolates using a highly discriminatory multilocus variable-number tandem-repeat analysis (MLVA) assay
title_fullStr	Genotyping Brucella canis isolates using a highly discriminatory multilocus variable-number tandem-repeat analysis (MLVA) assay
title_full_unstemmed	Genotyping Brucella canis isolates using a highly discriminatory multilocus variable-number tandem-repeat analysis (MLVA) assay
title_sort	genotyping brucella canis isolates using a highly discriminatory multilocus variable-number tandem-repeat analysis (mlva) assay
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/3461f6765e614c1387ad6e2d2a35190b
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Genotyping Brucella canis isolates using a highly discriminatory multilocus variable-number tandem-repeat analysis (MLVA) assay

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