Freezing adversely affects measurement of vascular endothelial growth factor levels in human aqueous samples

Sankarathi Balaiya Sandeep Grover Ravi K Murthy Kakarla V ChalamDepartment of Ophthalmology, University of Florida College of Medicine, Jacksonville, FL, USAPurpose: Aqueous levels of vascular endothelial growth factor (VEGF) can be a surrogate marker of intraocular VEGF activity and a measure of ef...

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Autores principales:	Sankarathi Balaiya, Sandeep Grover, Ravi K Murthy, et al
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2011
Materias:	Ophthalmology RE1-994
Acceso en línea:	https://doaj.org/article/346c615c06b24816aa26263477b2b4ed
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spelling	oai:doaj.org-article:346c615c06b24816aa26263477b2b4ed2021-12-02T07:42:39ZFreezing adversely affects measurement of vascular endothelial growth factor levels in human aqueous samples1177-54671177-5483https://doaj.org/article/346c615c06b24816aa26263477b2b4ed2011-01-01T00:00:00Zhttp://www.dovepress.com/freezing-adversely-affects-measurement-of-vascular-endothelial-growth--a6038https://doaj.org/toc/1177-5467https://doaj.org/toc/1177-5483Sankarathi Balaiya Sandeep Grover Ravi K Murthy Kakarla V ChalamDepartment of Ophthalmology, University of Florida College of Medicine, Jacksonville, FL, USAPurpose: Aqueous levels of vascular endothelial growth factor (VEGF) can be a surrogate marker of intraocular VEGF activity and a measure of efficacy of anti-VEGF treatment in a variety of vasoproliferative retinal disorders, including diabetic retinopathy, age-related macular degeneration, and central retinal vein occlusion. Measurement of the VEGF level may be adversely affected by premeasurement variables, such as freezing and delay, in sample analysis. We aim to evaluate the effect of storage and delayed measurement of human aqueous VEGF levels in these conditions.Methods: Aqueous samples collected from patients receiving intravitreal injection of bevacizumab for various retinal diseases were divided into two groups. In Group 1, the VEGF levels were analyzed on the same day; in Group 2, the VEGF levels were analyzed after 21 days of freezer storage (-80&deg;C) using immunobead assay. Statistical comparison using a paired t-test was performed between the two groups.Results: Thirty-one aqueous humor samples were collected, and the VEGF concentration for fresh samples was 7.8 &plusmn; 5.9 pg/mL (mean &plusmn; SD) compared to 6.5 &plusmn; 6.0 pg/mL in frozen samples, resulting in a statistically significant difference (P = 0.03).Conclusions: Accurate measurement of the VEGF level is a vital component of clinical decision-making. Delayed analysis of VEGF levels in aqueous samples may result in significant sample degradation and lower levels of measured VEGF.Keywords: VEGF level, aqueous humor, immunobead assay, VEGF storage Sankarathi BalaiyaSandeep GroverRavi K Murthyet alDove Medical PressarticleOphthalmologyRE1-994ENClinical Ophthalmology, Vol 2011, Iss default, Pp 81-85 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Ophthalmology RE1-994
spellingShingle	Ophthalmology RE1-994 Sankarathi Balaiya Sandeep Grover Ravi K Murthy et al Freezing adversely affects measurement of vascular endothelial growth factor levels in human aqueous samples
description	Sankarathi Balaiya Sandeep Grover Ravi K Murthy Kakarla V ChalamDepartment of Ophthalmology, University of Florida College of Medicine, Jacksonville, FL, USAPurpose: Aqueous levels of vascular endothelial growth factor (VEGF) can be a surrogate marker of intraocular VEGF activity and a measure of efficacy of anti-VEGF treatment in a variety of vasoproliferative retinal disorders, including diabetic retinopathy, age-related macular degeneration, and central retinal vein occlusion. Measurement of the VEGF level may be adversely affected by premeasurement variables, such as freezing and delay, in sample analysis. We aim to evaluate the effect of storage and delayed measurement of human aqueous VEGF levels in these conditions.Methods: Aqueous samples collected from patients receiving intravitreal injection of bevacizumab for various retinal diseases were divided into two groups. In Group 1, the VEGF levels were analyzed on the same day; in Group 2, the VEGF levels were analyzed after 21 days of freezer storage (-80&deg;C) using immunobead assay. Statistical comparison using a paired t-test was performed between the two groups.Results: Thirty-one aqueous humor samples were collected, and the VEGF concentration for fresh samples was 7.8 &plusmn; 5.9 pg/mL (mean &plusmn; SD) compared to 6.5 &plusmn; 6.0 pg/mL in frozen samples, resulting in a statistically significant difference (P = 0.03).Conclusions: Accurate measurement of the VEGF level is a vital component of clinical decision-making. Delayed analysis of VEGF levels in aqueous samples may result in significant sample degradation and lower levels of measured VEGF.Keywords: VEGF level, aqueous humor, immunobead assay, VEGF storage
format	article
author	Sankarathi Balaiya Sandeep Grover Ravi K Murthy et al
author_facet	Sankarathi Balaiya Sandeep Grover Ravi K Murthy et al
author_sort	Sankarathi Balaiya
title	Freezing adversely affects measurement of vascular endothelial growth factor levels in human aqueous samples
title_short	Freezing adversely affects measurement of vascular endothelial growth factor levels in human aqueous samples
title_full	Freezing adversely affects measurement of vascular endothelial growth factor levels in human aqueous samples
title_fullStr	Freezing adversely affects measurement of vascular endothelial growth factor levels in human aqueous samples
title_full_unstemmed	Freezing adversely affects measurement of vascular endothelial growth factor levels in human aqueous samples
title_sort	freezing adversely affects measurement of vascular endothelial growth factor levels in human aqueous samples
publisher	Dove Medical Press
publishDate	2011
url	https://doaj.org/article/346c615c06b24816aa26263477b2b4ed
work_keys_str_mv	AT sankarathibalaiya freezingadverselyaffectsmeasurementofvascularendothelialgrowthfactorlevelsinhumanaqueoussamples AT sandeepgrover freezingadverselyaffectsmeasurementofvascularendothelialgrowthfactorlevelsinhumanaqueoussamples AT ravikmurthy freezingadverselyaffectsmeasurementofvascularendothelialgrowthfactorlevelsinhumanaqueoussamples AT etal freezingadverselyaffectsmeasurementofvascularendothelialgrowthfactorlevelsinhumanaqueoussamples
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Freezing adversely affects measurement of vascular endothelial growth factor levels in human aqueous samples

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