Diagnosis of Q Fever and Brucellosis in Aborted Ovine Fetuses by Microbiological, Pathological and Immunohistochemical Methods

Brucellosis and Q fever, two zoonoses, are important causes of abortion in ruminants, as well as economically significant diseases caused by a gram-negative bacterium. Determination of these diseases is therefore of great importance. In this study, the organs of 35 naturally infected and aborted ovi...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Simten Yesilmen, Turan Yaman, Hakan Sağsöz, Servet Bademkiran
Formato:	article
Lenguaje:	EN
Publicado:	Sciendo 2018
Materias:	q fever brucella melitensis immunohistochemistry abortus Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/346d0219ba9243fb9a0bb0b5353123c3
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:346d0219ba9243fb9a0bb0b5353123c3
record_format	dspace
spelling	oai:doaj.org-article:346d0219ba9243fb9a0bb0b5353123c32021-11-17T21:27:52ZDiagnosis of Q Fever and Brucellosis in Aborted Ovine Fetuses by Microbiological, Pathological and Immunohistochemical Methods1820-744810.2478/acve-2018-0013https://doaj.org/article/346d0219ba9243fb9a0bb0b5353123c32018-06-01T00:00:00Zhttps://doi.org/10.2478/acve-2018-0013https://doaj.org/toc/1820-7448Brucellosis and Q fever, two zoonoses, are important causes of abortion in ruminants, as well as economically significant diseases caused by a gram-negative bacterium. Determination of these diseases is therefore of great importance. In this study, the organs of 35 naturally infected and aborted ovine fetuses were examined for the presence of changes resulting from infections by Brucella melitensis and Coxiella burnetii, according to macroscopic, bacteriological, histopathological and immunohistochemical methods. B. melitensis was observed in 21 cases, and C. burnetii was observed in 8 cases of the aborted ovine fetuses, and these were determined with immunohistochemical methods. Brucellosis was observed in 18 of the aborted ovine fetuses, and this was determined by microbiological methods. Negative (-) results were found for all of the other fetuses. The Brucella antigen was determined to be localized as intracytoplasmic in mainly alveolar macrophages, bronchi, bronchioles, glandular epithelial cells around bronchial glands, neutrophils, hepatocytes and Kupffer cells. The Coxiella antigen was found to be localized in the alveolar macrophages in the lungs, bronchi, bronchioles and alveolus, and in the cytoplasms of bronchial gland epithelial cells, and in the cytoplasms of hepatocytes and Kupffer cells in the liver. Immunohistochemical and microbiological diagnoses of brucellosis and coxiellosis were compared; it was concluded that immunohistochemical methods were more safely applied than microbiological methods.Simten YesilmenTuran YamanHakan SağsözServet BademkiranSciendoarticleq feverbrucella melitensisimmunohistochemistryabortusVeterinary medicineSF600-1100ENActa Veterinaria, Vol 68, Iss 2, Pp 168-177 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	q fever brucella melitensis immunohistochemistry abortus Veterinary medicine SF600-1100
spellingShingle	q fever brucella melitensis immunohistochemistry abortus Veterinary medicine SF600-1100 Simten Yesilmen Turan Yaman Hakan Sağsöz Servet Bademkiran Diagnosis of Q Fever and Brucellosis in Aborted Ovine Fetuses by Microbiological, Pathological and Immunohistochemical Methods
description	Brucellosis and Q fever, two zoonoses, are important causes of abortion in ruminants, as well as economically significant diseases caused by a gram-negative bacterium. Determination of these diseases is therefore of great importance. In this study, the organs of 35 naturally infected and aborted ovine fetuses were examined for the presence of changes resulting from infections by Brucella melitensis and Coxiella burnetii, according to macroscopic, bacteriological, histopathological and immunohistochemical methods. B. melitensis was observed in 21 cases, and C. burnetii was observed in 8 cases of the aborted ovine fetuses, and these were determined with immunohistochemical methods. Brucellosis was observed in 18 of the aborted ovine fetuses, and this was determined by microbiological methods. Negative (-) results were found for all of the other fetuses. The Brucella antigen was determined to be localized as intracytoplasmic in mainly alveolar macrophages, bronchi, bronchioles, glandular epithelial cells around bronchial glands, neutrophils, hepatocytes and Kupffer cells. The Coxiella antigen was found to be localized in the alveolar macrophages in the lungs, bronchi, bronchioles and alveolus, and in the cytoplasms of bronchial gland epithelial cells, and in the cytoplasms of hepatocytes and Kupffer cells in the liver. Immunohistochemical and microbiological diagnoses of brucellosis and coxiellosis were compared; it was concluded that immunohistochemical methods were more safely applied than microbiological methods.
format	article
author	Simten Yesilmen Turan Yaman Hakan Sağsöz Servet Bademkiran
author_facet	Simten Yesilmen Turan Yaman Hakan Sağsöz Servet Bademkiran
author_sort	Simten Yesilmen
title	Diagnosis of Q Fever and Brucellosis in Aborted Ovine Fetuses by Microbiological, Pathological and Immunohistochemical Methods
title_short	Diagnosis of Q Fever and Brucellosis in Aborted Ovine Fetuses by Microbiological, Pathological and Immunohistochemical Methods
title_full	Diagnosis of Q Fever and Brucellosis in Aborted Ovine Fetuses by Microbiological, Pathological and Immunohistochemical Methods
title_fullStr	Diagnosis of Q Fever and Brucellosis in Aborted Ovine Fetuses by Microbiological, Pathological and Immunohistochemical Methods
title_full_unstemmed	Diagnosis of Q Fever and Brucellosis in Aborted Ovine Fetuses by Microbiological, Pathological and Immunohistochemical Methods
title_sort	diagnosis of q fever and brucellosis in aborted ovine fetuses by microbiological, pathological and immunohistochemical methods
publisher	Sciendo
publishDate	2018
url	https://doaj.org/article/346d0219ba9243fb9a0bb0b5353123c3
work_keys_str_mv	AT simtenyesilmen diagnosisofqfeverandbrucellosisinabortedovinefetusesbymicrobiologicalpathologicalandimmunohistochemicalmethods AT turanyaman diagnosisofqfeverandbrucellosisinabortedovinefetusesbymicrobiologicalpathologicalandimmunohistochemicalmethods AT hakansagsoz diagnosisofqfeverandbrucellosisinabortedovinefetusesbymicrobiologicalpathologicalandimmunohistochemicalmethods AT servetbademkiran diagnosisofqfeverandbrucellosisinabortedovinefetusesbymicrobiologicalpathologicalandimmunohistochemicalmethods
_version_	1718425276477603840

Diagnosis of Q Fever and Brucellosis in Aborted Ovine Fetuses by Microbiological, Pathological and Immunohistochemical Methods

Ejemplares similares