Proteomic differences between focal and diffuse traumatic brain injury in human brain tissue

Abstract The early molecular response to severe traumatic brain injury (TBI) was evaluated using biopsies of structurally normal-appearing cortex, obtained at location for intracranial pressure (ICP) monitoring, from 16 severe TBI patients. Mass spectrometry (MS; label free and stable isotope dimeth...

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Autores principales:	Sami Abu Hamdeh, Ganna Shevchenko, Jia Mi, Sravani Musunuri, Jonas Bergquist, Niklas Marklund
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/346d2dc6689c4e9baa4edb88868b5204
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spelling	oai:doaj.org-article:346d2dc6689c4e9baa4edb88868b52042021-12-02T16:08:02ZProteomic differences between focal and diffuse traumatic brain injury in human brain tissue10.1038/s41598-018-25060-02045-2322https://doaj.org/article/346d2dc6689c4e9baa4edb88868b52042018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-25060-0https://doaj.org/toc/2045-2322Abstract The early molecular response to severe traumatic brain injury (TBI) was evaluated using biopsies of structurally normal-appearing cortex, obtained at location for intracranial pressure (ICP) monitoring, from 16 severe TBI patients. Mass spectrometry (MS; label free and stable isotope dimethyl labeling) quantitation proteomics showed a strikingly different molecular pattern in TBI in comparison to cortical biopsies from 11 idiopathic normal pressure hydrocephalus patients. Diffuse TBI showed increased expression of peptides related to neurodegeneration (Tau and Fascin, p < 0.05), reduced expression related to antioxidant defense (Glutathione S-transferase Mu 3, Peroxiredoxin-6, Thioredoxin-dependent peroxide reductase; p < 0.05) and increased expression of potential biomarkers (e.g. Neurogranin, Fatty acid-binding protein, heart p < 0.05) compared to focal TBI. Proteomics of human brain biopsies displayed considerable molecular heterogeneity among the different TBI subtypes with consequences for the pathophysiology and development of targeted treatments for TBI.Sami Abu HamdehGanna ShevchenkoJia MiSravani MusunuriJonas BergquistNiklas MarklundNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-15 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sami Abu Hamdeh Ganna Shevchenko Jia Mi Sravani Musunuri Jonas Bergquist Niklas Marklund Proteomic differences between focal and diffuse traumatic brain injury in human brain tissue
description	Abstract The early molecular response to severe traumatic brain injury (TBI) was evaluated using biopsies of structurally normal-appearing cortex, obtained at location for intracranial pressure (ICP) monitoring, from 16 severe TBI patients. Mass spectrometry (MS; label free and stable isotope dimethyl labeling) quantitation proteomics showed a strikingly different molecular pattern in TBI in comparison to cortical biopsies from 11 idiopathic normal pressure hydrocephalus patients. Diffuse TBI showed increased expression of peptides related to neurodegeneration (Tau and Fascin, p < 0.05), reduced expression related to antioxidant defense (Glutathione S-transferase Mu 3, Peroxiredoxin-6, Thioredoxin-dependent peroxide reductase; p < 0.05) and increased expression of potential biomarkers (e.g. Neurogranin, Fatty acid-binding protein, heart p < 0.05) compared to focal TBI. Proteomics of human brain biopsies displayed considerable molecular heterogeneity among the different TBI subtypes with consequences for the pathophysiology and development of targeted treatments for TBI.
format	article
author	Sami Abu Hamdeh Ganna Shevchenko Jia Mi Sravani Musunuri Jonas Bergquist Niklas Marklund
author_facet	Sami Abu Hamdeh Ganna Shevchenko Jia Mi Sravani Musunuri Jonas Bergquist Niklas Marklund
author_sort	Sami Abu Hamdeh
title	Proteomic differences between focal and diffuse traumatic brain injury in human brain tissue
title_short	Proteomic differences between focal and diffuse traumatic brain injury in human brain tissue
title_full	Proteomic differences between focal and diffuse traumatic brain injury in human brain tissue
title_fullStr	Proteomic differences between focal and diffuse traumatic brain injury in human brain tissue
title_full_unstemmed	Proteomic differences between focal and diffuse traumatic brain injury in human brain tissue
title_sort	proteomic differences between focal and diffuse traumatic brain injury in human brain tissue
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/346d2dc6689c4e9baa4edb88868b5204
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Proteomic differences between focal and diffuse traumatic brain injury in human brain tissue

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