Sperm-binding regions on bovine egg zona pellucida glycoprotein ZP4 studied in a solid supported form on plastic plate.

The zona pellucida (ZP) is a transparent envelope that surrounds the mammalian oocyte and mediates species-selective sperm-oocyte interactions. The bovine ZP consists of the glycoproteins ZP2, ZP3, and ZP4. Sperm-binding mechanisms of the bovine ZP are not yet fully elucidated. In a previous report,...

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Autores principales:	Kamila Dilimulati, Misaki Orita, Ganbat Undram, Naoto Yonezawa
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3471341580d240e4a53c8bab223ff46a
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spelling	oai:doaj.org-article:3471341580d240e4a53c8bab223ff46a2021-12-02T20:09:22ZSperm-binding regions on bovine egg zona pellucida glycoprotein ZP4 studied in a solid supported form on plastic plate.1932-620310.1371/journal.pone.0254234https://doaj.org/article/3471341580d240e4a53c8bab223ff46a2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0254234https://doaj.org/toc/1932-6203The zona pellucida (ZP) is a transparent envelope that surrounds the mammalian oocyte and mediates species-selective sperm-oocyte interactions. The bovine ZP consists of the glycoproteins ZP2, ZP3, and ZP4. Sperm-binding mechanisms of the bovine ZP are not yet fully elucidated. In a previous report, we established the expression system of bovine ZP glycoproteins using Sf9 insect cells and found that the ZP3/ZP4 heterocomplex inhibits the binding of sperm to the ZP in a competitive inhibition assay, while ZP2, ZP3, ZP4, the ZP2/ZP3 complex, and the ZP2/ZP4 complex do not exhibit this activity. Here, we show that bovine sperm binds to plastic plates coated with ZP4 in the absence of ZP3. We made a series of ZP4 deletion mutants to study the sperm-binding sites. The N-terminal region, Lys-25 to Asp-136, and the middle region, Ser-290 to Lys-340, of ZP4 exhibit sperm-binding activity. These results suggest that among the three components of bovine ZP glycoproteins, ZP4 contains the major potential sperm-binding sites, and the formation of a multivalent complex is necessary for the sperm-binding activity of ZP4.Kamila DilimulatiMisaki OritaGanbat UndramNaoto YonezawaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 7, p e0254234 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kamila Dilimulati Misaki Orita Ganbat Undram Naoto Yonezawa Sperm-binding regions on bovine egg zona pellucida glycoprotein ZP4 studied in a solid supported form on plastic plate.
description	The zona pellucida (ZP) is a transparent envelope that surrounds the mammalian oocyte and mediates species-selective sperm-oocyte interactions. The bovine ZP consists of the glycoproteins ZP2, ZP3, and ZP4. Sperm-binding mechanisms of the bovine ZP are not yet fully elucidated. In a previous report, we established the expression system of bovine ZP glycoproteins using Sf9 insect cells and found that the ZP3/ZP4 heterocomplex inhibits the binding of sperm to the ZP in a competitive inhibition assay, while ZP2, ZP3, ZP4, the ZP2/ZP3 complex, and the ZP2/ZP4 complex do not exhibit this activity. Here, we show that bovine sperm binds to plastic plates coated with ZP4 in the absence of ZP3. We made a series of ZP4 deletion mutants to study the sperm-binding sites. The N-terminal region, Lys-25 to Asp-136, and the middle region, Ser-290 to Lys-340, of ZP4 exhibit sperm-binding activity. These results suggest that among the three components of bovine ZP glycoproteins, ZP4 contains the major potential sperm-binding sites, and the formation of a multivalent complex is necessary for the sperm-binding activity of ZP4.
format	article
author	Kamila Dilimulati Misaki Orita Ganbat Undram Naoto Yonezawa
author_facet	Kamila Dilimulati Misaki Orita Ganbat Undram Naoto Yonezawa
author_sort	Kamila Dilimulati
title	Sperm-binding regions on bovine egg zona pellucida glycoprotein ZP4 studied in a solid supported form on plastic plate.
title_short	Sperm-binding regions on bovine egg zona pellucida glycoprotein ZP4 studied in a solid supported form on plastic plate.
title_full	Sperm-binding regions on bovine egg zona pellucida glycoprotein ZP4 studied in a solid supported form on plastic plate.
title_fullStr	Sperm-binding regions on bovine egg zona pellucida glycoprotein ZP4 studied in a solid supported form on plastic plate.
title_full_unstemmed	Sperm-binding regions on bovine egg zona pellucida glycoprotein ZP4 studied in a solid supported form on plastic plate.
title_sort	sperm-binding regions on bovine egg zona pellucida glycoprotein zp4 studied in a solid supported form on plastic plate.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/3471341580d240e4a53c8bab223ff46a
work_keys_str_mv	AT kamiladilimulati spermbindingregionsonbovineeggzonapellucidaglycoproteinzp4studiedinasolidsupportedformonplasticplate AT misakiorita spermbindingregionsonbovineeggzonapellucidaglycoproteinzp4studiedinasolidsupportedformonplasticplate AT ganbatundram spermbindingregionsonbovineeggzonapellucidaglycoproteinzp4studiedinasolidsupportedformonplasticplate AT naotoyonezawa spermbindingregionsonbovineeggzonapellucidaglycoproteinzp4studiedinasolidsupportedformonplasticplate
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Sperm-binding regions on bovine egg zona pellucida glycoprotein ZP4 studied in a solid supported form on plastic plate.

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