Overexpression of Pea DNA Helicase 45 (PDH45) imparts tolerance to multiple abiotic stresses in chili (Capsicum annuum L.)

Abstract Imparting tolerance to abiotic stresses is of global importance as they inflict significant yield losses in field as well as in vegetable crops. Transcriptional activators, including helicases are identified to play a pivotal role in stress mitigation. Helicases, also known as molecular mot...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Tagginahalli N. Shivakumara, Rohini Sreevathsa, Prasanta K. Dash, M. S. Sheshshayee, Pradeep K. Papolu, Uma Rao, Narendra Tuteja, M. UdayaKumar
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/34752b783749499cb49eccc58313405b
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:34752b783749499cb49eccc58313405b
record_format	dspace
spelling	oai:doaj.org-article:34752b783749499cb49eccc58313405b2021-12-02T12:32:54ZOverexpression of Pea DNA Helicase 45 (PDH45) imparts tolerance to multiple abiotic stresses in chili (Capsicum annuum L.)10.1038/s41598-017-02589-02045-2322https://doaj.org/article/34752b783749499cb49eccc58313405b2017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02589-0https://doaj.org/toc/2045-2322Abstract Imparting tolerance to abiotic stresses is of global importance as they inflict significant yield losses in field as well as in vegetable crops. Transcriptional activators, including helicases are identified to play a pivotal role in stress mitigation. Helicases, also known as molecular motors, are involved in myriad cellular processes that impart intrinsic tolerance to abiotic stresses in plants. Our study demonstrates the potential of a Pea DNA Helicase 45 (PDH45), in combating multiple abiotic stresses in chili. We harnessed Agrobacterium-mediated in planta transformation strategy for the generation of stable, single copy transgenic events. Precise molecular detection of the transgenes by sqRT-PCR coupled with genomic Southern analysis revealed variation in the integration of PDH45 at distinct loci in independent transgenic events. Characterization of five promising transgenic events showed both improved response to an array of simulated abiotic stresses and enhanced expression of several stress-responsive genes. While survival and recovery of transgenic events were significantly higher under gradual moisture stress conditions, under imposition of moderate stress, the transgenic events exhibited invigorated growth and productivity with concomitant improvement in water use efficiency (WUE). Thus, our study, unequivocally demonstrated the cardinal role of PDH45 in alleviating multiple abiotic stresses in chili.Tagginahalli N. ShivakumaraRohini SreevathsaPrasanta K. DashM. S. SheshshayeePradeep K. PapoluUma RaoNarendra TutejaM. UdayaKumarNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tagginahalli N. Shivakumara Rohini Sreevathsa Prasanta K. Dash M. S. Sheshshayee Pradeep K. Papolu Uma Rao Narendra Tuteja M. UdayaKumar Overexpression of Pea DNA Helicase 45 (PDH45) imparts tolerance to multiple abiotic stresses in chili (Capsicum annuum L.)
description	Abstract Imparting tolerance to abiotic stresses is of global importance as they inflict significant yield losses in field as well as in vegetable crops. Transcriptional activators, including helicases are identified to play a pivotal role in stress mitigation. Helicases, also known as molecular motors, are involved in myriad cellular processes that impart intrinsic tolerance to abiotic stresses in plants. Our study demonstrates the potential of a Pea DNA Helicase 45 (PDH45), in combating multiple abiotic stresses in chili. We harnessed Agrobacterium-mediated in planta transformation strategy for the generation of stable, single copy transgenic events. Precise molecular detection of the transgenes by sqRT-PCR coupled with genomic Southern analysis revealed variation in the integration of PDH45 at distinct loci in independent transgenic events. Characterization of five promising transgenic events showed both improved response to an array of simulated abiotic stresses and enhanced expression of several stress-responsive genes. While survival and recovery of transgenic events were significantly higher under gradual moisture stress conditions, under imposition of moderate stress, the transgenic events exhibited invigorated growth and productivity with concomitant improvement in water use efficiency (WUE). Thus, our study, unequivocally demonstrated the cardinal role of PDH45 in alleviating multiple abiotic stresses in chili.
format	article
author	Tagginahalli N. Shivakumara Rohini Sreevathsa Prasanta K. Dash M. S. Sheshshayee Pradeep K. Papolu Uma Rao Narendra Tuteja M. UdayaKumar
author_facet	Tagginahalli N. Shivakumara Rohini Sreevathsa Prasanta K. Dash M. S. Sheshshayee Pradeep K. Papolu Uma Rao Narendra Tuteja M. UdayaKumar
author_sort	Tagginahalli N. Shivakumara
title	Overexpression of Pea DNA Helicase 45 (PDH45) imparts tolerance to multiple abiotic stresses in chili (Capsicum annuum L.)
title_short	Overexpression of Pea DNA Helicase 45 (PDH45) imparts tolerance to multiple abiotic stresses in chili (Capsicum annuum L.)
title_full	Overexpression of Pea DNA Helicase 45 (PDH45) imparts tolerance to multiple abiotic stresses in chili (Capsicum annuum L.)
title_fullStr	Overexpression of Pea DNA Helicase 45 (PDH45) imparts tolerance to multiple abiotic stresses in chili (Capsicum annuum L.)
title_full_unstemmed	Overexpression of Pea DNA Helicase 45 (PDH45) imparts tolerance to multiple abiotic stresses in chili (Capsicum annuum L.)
title_sort	overexpression of pea dna helicase 45 (pdh45) imparts tolerance to multiple abiotic stresses in chili (capsicum annuum l.)
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/34752b783749499cb49eccc58313405b
work_keys_str_mv	AT tagginahallinshivakumara overexpressionofpeadnahelicase45pdh45impartstolerancetomultipleabioticstressesinchilicapsicumannuuml AT rohinisreevathsa overexpressionofpeadnahelicase45pdh45impartstolerancetomultipleabioticstressesinchilicapsicumannuuml AT prasantakdash overexpressionofpeadnahelicase45pdh45impartstolerancetomultipleabioticstressesinchilicapsicumannuuml AT mssheshshayee overexpressionofpeadnahelicase45pdh45impartstolerancetomultipleabioticstressesinchilicapsicumannuuml AT pradeepkpapolu overexpressionofpeadnahelicase45pdh45impartstolerancetomultipleabioticstressesinchilicapsicumannuuml AT umarao overexpressionofpeadnahelicase45pdh45impartstolerancetomultipleabioticstressesinchilicapsicumannuuml AT narendratuteja overexpressionofpeadnahelicase45pdh45impartstolerancetomultipleabioticstressesinchilicapsicumannuuml AT mudayakumar overexpressionofpeadnahelicase45pdh45impartstolerancetomultipleabioticstressesinchilicapsicumannuuml
_version_	1718393910632382464

Overexpression of Pea DNA Helicase 45 (PDH45) imparts tolerance to multiple abiotic stresses in chili (Capsicum annuum L.)

Ejemplares similares