The SCO1731 methyltransferase modulates actinorhodin production and morphological differentiation of Streptomyces coelicolor A3(2)

Abstract Streptomyces coelicolor is a Gram-positive microorganism often used as a model of physiological and morphological differentiation in streptomycetes, prolific producers of secondary metabolites with important biological activities. In the present study, we analysed Streptomyces coelicolor gr...

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Autores principales:	Annalisa Pisciotta, Angel Manteca, Rosa Alduina
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Actinorhodin Production Tn5 Mutant Strains Hypomethylating Agents Decitabine Cytosine Methylation Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3479baa351734cc1954db38c8f45515f
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spelling	oai:doaj.org-article:3479baa351734cc1954db38c8f45515f2021-12-02T15:08:10ZThe SCO1731 methyltransferase modulates actinorhodin production and morphological differentiation of Streptomyces coelicolor A3(2)10.1038/s41598-018-32027-82045-2322https://doaj.org/article/3479baa351734cc1954db38c8f45515f2018-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-32027-8https://doaj.org/toc/2045-2322Abstract Streptomyces coelicolor is a Gram-positive microorganism often used as a model of physiological and morphological differentiation in streptomycetes, prolific producers of secondary metabolites with important biological activities. In the present study, we analysed Streptomyces coelicolor growth and differentiation in the presence of the hypo-methylating agent 5′-aza-2′-deoxycytidine (5-aza-dC) in order to investigate whether cytosine methylation has a role in differentiation. We found that cytosine demethylation caused a delay in spore germination, aerial mycelium development, sporulation, as well as a massive impairment of actinorhodin production. Thus, we searched for putative DNA methyltransferase genes in the genome and constructed a mutant of the SCO1731 gene. The analysis of the SCO1731::Tn5062 mutant strain demonstrated that inactivation of SCO1731 leads to a strong decrease of cytosine methylation and almost to the same phenotype obtained after 5-aza-dC treatment. Altogether, our data demonstrate that cytosine methylation influences morphological differentiation and actinorhodin production in S. coelicolor and expand our knowledge on this model bacterial system.Annalisa PisciottaAngel MantecaRosa AlduinaNature PortfolioarticleActinorhodin ProductionTn5 Mutant StrainsHypomethylating AgentsDecitabineCytosine MethylationMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-11 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Actinorhodin Production Tn5 Mutant Strains Hypomethylating Agents Decitabine Cytosine Methylation Medicine R Science Q
spellingShingle	Actinorhodin Production Tn5 Mutant Strains Hypomethylating Agents Decitabine Cytosine Methylation Medicine R Science Q Annalisa Pisciotta Angel Manteca Rosa Alduina The SCO1731 methyltransferase modulates actinorhodin production and morphological differentiation of Streptomyces coelicolor A3(2)
description	Abstract Streptomyces coelicolor is a Gram-positive microorganism often used as a model of physiological and morphological differentiation in streptomycetes, prolific producers of secondary metabolites with important biological activities. In the present study, we analysed Streptomyces coelicolor growth and differentiation in the presence of the hypo-methylating agent 5′-aza-2′-deoxycytidine (5-aza-dC) in order to investigate whether cytosine methylation has a role in differentiation. We found that cytosine demethylation caused a delay in spore germination, aerial mycelium development, sporulation, as well as a massive impairment of actinorhodin production. Thus, we searched for putative DNA methyltransferase genes in the genome and constructed a mutant of the SCO1731 gene. The analysis of the SCO1731::Tn5062 mutant strain demonstrated that inactivation of SCO1731 leads to a strong decrease of cytosine methylation and almost to the same phenotype obtained after 5-aza-dC treatment. Altogether, our data demonstrate that cytosine methylation influences morphological differentiation and actinorhodin production in S. coelicolor and expand our knowledge on this model bacterial system.
format	article
author	Annalisa Pisciotta Angel Manteca Rosa Alduina
author_facet	Annalisa Pisciotta Angel Manteca Rosa Alduina
author_sort	Annalisa Pisciotta
title	The SCO1731 methyltransferase modulates actinorhodin production and morphological differentiation of Streptomyces coelicolor A3(2)
title_short	The SCO1731 methyltransferase modulates actinorhodin production and morphological differentiation of Streptomyces coelicolor A3(2)
title_full	The SCO1731 methyltransferase modulates actinorhodin production and morphological differentiation of Streptomyces coelicolor A3(2)
title_fullStr	The SCO1731 methyltransferase modulates actinorhodin production and morphological differentiation of Streptomyces coelicolor A3(2)
title_full_unstemmed	The SCO1731 methyltransferase modulates actinorhodin production and morphological differentiation of Streptomyces coelicolor A3(2)
title_sort	sco1731 methyltransferase modulates actinorhodin production and morphological differentiation of streptomyces coelicolor a3(2)
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/3479baa351734cc1954db38c8f45515f
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The SCO1731 methyltransferase modulates actinorhodin production and morphological differentiation of Streptomyces coelicolor A3(2)

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