Antibiotic-Loaded MMT/PLL-Based Coating on the Surface of Endosseous Implants to Suppress Bacterial Infections

Xingfang Yu,1,* Xin Liao,2,* Hongwei Chen1 1Department of Orthopedics, The Affiliated Yiwu Hospital of Wenzhou Medical University, Yiwu, Zhejiang, 322000, People’s Republic of China; 2Department of Orthopedics, The Second Affiliated Hospital and Yuying Children’s Hospital of Wenz...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Yu X, Liao X, Chen H
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	bone infections multilayer films microenvironment layer-by-layer antibacterial Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/348066111d5148cf908fdc0e7ff519a7
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:348066111d5148cf908fdc0e7ff519a7
record_format	dspace
spelling	oai:doaj.org-article:348066111d5148cf908fdc0e7ff519a72021-12-02T15:39:46ZAntibiotic-Loaded MMT/PLL-Based Coating on the Surface of Endosseous Implants to Suppress Bacterial Infections1178-2013https://doaj.org/article/348066111d5148cf908fdc0e7ff519a72021-04-01T00:00:00Zhttps://www.dovepress.com/antibiotic-loaded-mmtpll-based-coating-on-the-surface-of-endosseous-im-peer-reviewed-fulltext-article-IJNhttps://doaj.org/toc/1178-2013Xingfang Yu,1,* Xin Liao,2,* Hongwei Chen1 1Department of Orthopedics, The Affiliated Yiwu Hospital of Wenzhou Medical University, Yiwu, Zhejiang, 322000, People’s Republic of China; 2Department of Orthopedics, The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, 325000, People’s Republic of China*These authors contributed equally to this workCorrespondence: Hongwei ChenDepartment of Orthopedics, The Affiliated Yiwu Hospital of Wenzhou Medical University, Yiwu, Zhejiang, 322000, People’s Republic of ChinaTel +86-13506896988Email sssaaa18@163.comBackground: Bone infections remain one of the most common and serious complications of orthopedic surgery, posing a tremendous economic burden to society and patients. This is because bacteria colonize and multiply on the surface of the implant. The (MMT/PLL)8 multilayer films have been shown to effectively release antibiotics depending on the changes in the microenvironment. Here, vancomycin was loaded into the (MMT/PLL)8 multilayer films, which were prepared to be used as a local delivery system for the treatment of bone infections.Methods: We used the layer-by-layer self-assembly method to prepare VA-loaded coatings (MMT/PLL-VA)8 consisting of montmorillonite (MMT), poly-L-lysine (PLL), and VA. The thickness and surface morphology of coatings were characterized using spectroscopic ellipsometry and scanning electron microscopy (SEM). In order to evaluate the drug release behavior from coatings in different media, we measured the size of the zone of inhibition. Additionally, in vitro antibacterial activity was assessed using the shake-flask culture method and SEM images, while that of in vivo was evaluated by establishing an animal model of bone infection.Results: Our findings revealed that small-molecule antibiotics were successfully loaded into the (MMT/PLL-VA)8 multilayer film structure during the hierarchical self-assembly process and subsequently the multilayer film structure depicted linear growth behavior. The PLL in the multilayer films was progressively degraded which triggered the VA release when contacted with CMS or bacterial infections. The release of VA from multilayer film structure depends on the concentration changes of CMS. Notably, the multilayer films presented great in vitro cell compatibility. Moreover, the prepared antibacterial multilayer films showed excellent antibacterial property by killing more than 99.99% of S. aureus in 24 h. More importantly, we found that multilayer film exhibits good sterilization effect and biocompatibility under the stimulation of bacterial liquid both in vitro and in vivo antibacterial ability tests.Conclusion: Altogether, this study shows that (MMT/PLL-VA)8 multilayer films containing CMS and bacteria-responsive drug release properties posess high bactericidal activity and good biocompatibility. This finding provides a novel strategy for the treatment of bone infections.Keywords: bone infections, multilayer films, microenvironment, layer-by-layer, antibacterialYu XLiao XChen HDove Medical Pressarticlebone infectionsmultilayer filmsmicroenvironmentlayer-by-layerantibacterialMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 16, Pp 2983-2994 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	bone infections multilayer films microenvironment layer-by-layer antibacterial Medicine (General) R5-920
spellingShingle	bone infections multilayer films microenvironment layer-by-layer antibacterial Medicine (General) R5-920 Yu X Liao X Chen H Antibiotic-Loaded MMT/PLL-Based Coating on the Surface of Endosseous Implants to Suppress Bacterial Infections
description	Xingfang Yu,1,* Xin Liao,2,* Hongwei Chen1 1Department of Orthopedics, The Affiliated Yiwu Hospital of Wenzhou Medical University, Yiwu, Zhejiang, 322000, People’s Republic of China; 2Department of Orthopedics, The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, 325000, People’s Republic of China*These authors contributed equally to this workCorrespondence: Hongwei ChenDepartment of Orthopedics, The Affiliated Yiwu Hospital of Wenzhou Medical University, Yiwu, Zhejiang, 322000, People’s Republic of ChinaTel +86-13506896988Email sssaaa18@163.comBackground: Bone infections remain one of the most common and serious complications of orthopedic surgery, posing a tremendous economic burden to society and patients. This is because bacteria colonize and multiply on the surface of the implant. The (MMT/PLL)8 multilayer films have been shown to effectively release antibiotics depending on the changes in the microenvironment. Here, vancomycin was loaded into the (MMT/PLL)8 multilayer films, which were prepared to be used as a local delivery system for the treatment of bone infections.Methods: We used the layer-by-layer self-assembly method to prepare VA-loaded coatings (MMT/PLL-VA)8 consisting of montmorillonite (MMT), poly-L-lysine (PLL), and VA. The thickness and surface morphology of coatings were characterized using spectroscopic ellipsometry and scanning electron microscopy (SEM). In order to evaluate the drug release behavior from coatings in different media, we measured the size of the zone of inhibition. Additionally, in vitro antibacterial activity was assessed using the shake-flask culture method and SEM images, while that of in vivo was evaluated by establishing an animal model of bone infection.Results: Our findings revealed that small-molecule antibiotics were successfully loaded into the (MMT/PLL-VA)8 multilayer film structure during the hierarchical self-assembly process and subsequently the multilayer film structure depicted linear growth behavior. The PLL in the multilayer films was progressively degraded which triggered the VA release when contacted with CMS or bacterial infections. The release of VA from multilayer film structure depends on the concentration changes of CMS. Notably, the multilayer films presented great in vitro cell compatibility. Moreover, the prepared antibacterial multilayer films showed excellent antibacterial property by killing more than 99.99% of S. aureus in 24 h. More importantly, we found that multilayer film exhibits good sterilization effect and biocompatibility under the stimulation of bacterial liquid both in vitro and in vivo antibacterial ability tests.Conclusion: Altogether, this study shows that (MMT/PLL-VA)8 multilayer films containing CMS and bacteria-responsive drug release properties posess high bactericidal activity and good biocompatibility. This finding provides a novel strategy for the treatment of bone infections.Keywords: bone infections, multilayer films, microenvironment, layer-by-layer, antibacterial
format	article
author	Yu X Liao X Chen H
author_facet	Yu X Liao X Chen H
author_sort	Yu X
title	Antibiotic-Loaded MMT/PLL-Based Coating on the Surface of Endosseous Implants to Suppress Bacterial Infections
title_short	Antibiotic-Loaded MMT/PLL-Based Coating on the Surface of Endosseous Implants to Suppress Bacterial Infections
title_full	Antibiotic-Loaded MMT/PLL-Based Coating on the Surface of Endosseous Implants to Suppress Bacterial Infections
title_fullStr	Antibiotic-Loaded MMT/PLL-Based Coating on the Surface of Endosseous Implants to Suppress Bacterial Infections
title_full_unstemmed	Antibiotic-Loaded MMT/PLL-Based Coating on the Surface of Endosseous Implants to Suppress Bacterial Infections
title_sort	antibiotic-loaded mmt/pll-based coating on the surface of endosseous implants to suppress bacterial infections
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/348066111d5148cf908fdc0e7ff519a7
work_keys_str_mv	AT yux antibioticloadedmmtpllbasedcoatingonthesurfaceofendosseousimplantstosuppressbacterialinfections AT liaox antibioticloadedmmtpllbasedcoatingonthesurfaceofendosseousimplantstosuppressbacterialinfections AT chenh antibioticloadedmmtpllbasedcoatingonthesurfaceofendosseousimplantstosuppressbacterialinfections
_version_	1718385864501886976

Antibiotic-Loaded MMT/PLL-Based Coating on the Surface of Endosseous Implants to Suppress Bacterial Infections

Ejemplares similares