Highly Stabilized Core-Satellite Gold Nanoassemblies in Vivo: DNA-Directed Self-Assembly, PEG Modification and Cell Imaging

Abstract Au nanoparticles (NPs) have important applications in bioimaging, clinical diagnosis and even therapy due to its water-solubility, easy modification and drug-loaded capability, however, easy aggregation of Au NPs in normal saline and serum greatly limits its applications. In this work, high...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Liangfeng Tang, Guiping Yu, Lishan Tan, Min Li, Xiulong Deng, Jianyu Liu, Aiqing Li, Xuandi Lai, Jianqiang Hu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3481241e0e0c4a6692cc8a3f74ae68a9
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:3481241e0e0c4a6692cc8a3f74ae68a9
record_format	dspace
spelling	oai:doaj.org-article:3481241e0e0c4a6692cc8a3f74ae68a92021-12-02T11:52:59ZHighly Stabilized Core-Satellite Gold Nanoassemblies in Vivo: DNA-Directed Self-Assembly, PEG Modification and Cell Imaging10.1038/s41598-017-08903-02045-2322https://doaj.org/article/3481241e0e0c4a6692cc8a3f74ae68a92017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08903-0https://doaj.org/toc/2045-2322Abstract Au nanoparticles (NPs) have important applications in bioimaging, clinical diagnosis and even therapy due to its water-solubility, easy modification and drug-loaded capability, however, easy aggregation of Au NPs in normal saline and serum greatly limits its applications. In this work, highly stabilized core-satellite Au nanoassemblies (CSAuNAs) were constructed by a hierarchical DNA-directed self-assembly strategy, in which satellite Au NPs number could be effectively tuned through varying the ratios of core-AuNPs-ssDNA and satellite-AuNPs-ssDNAc. It was especially interesting that PEG-functionalized CSAuNAs (PEG-CSAuNAs) could not only bear saline solution but also resist the enzymatic degradation in fetal calf serum. Moreover, cell targeting and imaging indicated that the PEG-CSAuNAs had promising biotargeting and bioimaging capability. Finally, fluorescence imaging in vivo revealed that PEG-CSAuNAs modified with N-acetylation chitosan (CSNA) could be selectively accumulate in the kidneys with satisfactory renal retention capability. Therefore, the highly stabilized PEG-CSAuNAs open a new avenue for its applications in vivo.Liangfeng TangGuiping YuLishan TanMin LiXiulong DengJianyu LiuAiqing LiXuandi LaiJianqiang HuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Liangfeng Tang Guiping Yu Lishan Tan Min Li Xiulong Deng Jianyu Liu Aiqing Li Xuandi Lai Jianqiang Hu Highly Stabilized Core-Satellite Gold Nanoassemblies in Vivo: DNA-Directed Self-Assembly, PEG Modification and Cell Imaging
description	Abstract Au nanoparticles (NPs) have important applications in bioimaging, clinical diagnosis and even therapy due to its water-solubility, easy modification and drug-loaded capability, however, easy aggregation of Au NPs in normal saline and serum greatly limits its applications. In this work, highly stabilized core-satellite Au nanoassemblies (CSAuNAs) were constructed by a hierarchical DNA-directed self-assembly strategy, in which satellite Au NPs number could be effectively tuned through varying the ratios of core-AuNPs-ssDNA and satellite-AuNPs-ssDNAc. It was especially interesting that PEG-functionalized CSAuNAs (PEG-CSAuNAs) could not only bear saline solution but also resist the enzymatic degradation in fetal calf serum. Moreover, cell targeting and imaging indicated that the PEG-CSAuNAs had promising biotargeting and bioimaging capability. Finally, fluorescence imaging in vivo revealed that PEG-CSAuNAs modified with N-acetylation chitosan (CSNA) could be selectively accumulate in the kidneys with satisfactory renal retention capability. Therefore, the highly stabilized PEG-CSAuNAs open a new avenue for its applications in vivo.
format	article
author	Liangfeng Tang Guiping Yu Lishan Tan Min Li Xiulong Deng Jianyu Liu Aiqing Li Xuandi Lai Jianqiang Hu
author_facet	Liangfeng Tang Guiping Yu Lishan Tan Min Li Xiulong Deng Jianyu Liu Aiqing Li Xuandi Lai Jianqiang Hu
author_sort	Liangfeng Tang
title	Highly Stabilized Core-Satellite Gold Nanoassemblies in Vivo: DNA-Directed Self-Assembly, PEG Modification and Cell Imaging
title_short	Highly Stabilized Core-Satellite Gold Nanoassemblies in Vivo: DNA-Directed Self-Assembly, PEG Modification and Cell Imaging
title_full	Highly Stabilized Core-Satellite Gold Nanoassemblies in Vivo: DNA-Directed Self-Assembly, PEG Modification and Cell Imaging
title_fullStr	Highly Stabilized Core-Satellite Gold Nanoassemblies in Vivo: DNA-Directed Self-Assembly, PEG Modification and Cell Imaging
title_full_unstemmed	Highly Stabilized Core-Satellite Gold Nanoassemblies in Vivo: DNA-Directed Self-Assembly, PEG Modification and Cell Imaging
title_sort	highly stabilized core-satellite gold nanoassemblies in vivo: dna-directed self-assembly, peg modification and cell imaging
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/3481241e0e0c4a6692cc8a3f74ae68a9
work_keys_str_mv	AT liangfengtang highlystabilizedcoresatellitegoldnanoassembliesinvivodnadirectedselfassemblypegmodificationandcellimaging AT guipingyu highlystabilizedcoresatellitegoldnanoassembliesinvivodnadirectedselfassemblypegmodificationandcellimaging AT lishantan highlystabilizedcoresatellitegoldnanoassembliesinvivodnadirectedselfassemblypegmodificationandcellimaging AT minli highlystabilizedcoresatellitegoldnanoassembliesinvivodnadirectedselfassemblypegmodificationandcellimaging AT xiulongdeng highlystabilizedcoresatellitegoldnanoassembliesinvivodnadirectedselfassemblypegmodificationandcellimaging AT jianyuliu highlystabilizedcoresatellitegoldnanoassembliesinvivodnadirectedselfassemblypegmodificationandcellimaging AT aiqingli highlystabilizedcoresatellitegoldnanoassembliesinvivodnadirectedselfassemblypegmodificationandcellimaging AT xuandilai highlystabilizedcoresatellitegoldnanoassembliesinvivodnadirectedselfassemblypegmodificationandcellimaging AT jianqianghu highlystabilizedcoresatellitegoldnanoassembliesinvivodnadirectedselfassemblypegmodificationandcellimaging
_version_	1718394917186699264

Highly Stabilized Core-Satellite Gold Nanoassemblies in Vivo: DNA-Directed Self-Assembly, PEG Modification and Cell Imaging

Ejemplares similares