Multiple Compact Camera Fluorescence Detector for Real-Time PCR Devices

The polymerase chain reaction is an important technique in biological research because it tests for diseases with a small amount of DNA. However, this process is time consuming and can lead to sample contamination. Recently, real-time PCR techniques have emerged which make it possible to monitor the...

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Autores principales:	Seul-Bit-Na Koo, Hyeon-Gyu Chi, Jong-Dae Kim, Yu-Seop Kim, Ji-Sung Park, Chan-Young Park, Deuk-Ju Lee
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	real-time PCR fluorescence detection open platform image processing Chemical technology TP1-1185
Acceso en línea:	https://doaj.org/article/3485a57b4dc24d898d7f4df42ddf4ab6
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id	oai:doaj.org-article:3485a57b4dc24d898d7f4df42ddf4ab6
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spelling	oai:doaj.org-article:3485a57b4dc24d898d7f4df42ddf4ab62021-11-11T19:03:03ZMultiple Compact Camera Fluorescence Detector for Real-Time PCR Devices10.3390/s212170131424-8220https://doaj.org/article/3485a57b4dc24d898d7f4df42ddf4ab62021-10-01T00:00:00Zhttps://www.mdpi.com/1424-8220/21/21/7013https://doaj.org/toc/1424-8220The polymerase chain reaction is an important technique in biological research because it tests for diseases with a small amount of DNA. However, this process is time consuming and can lead to sample contamination. Recently, real-time PCR techniques have emerged which make it possible to monitor the amplification process for each cycle in real time. Existing camera-based systems that measure fluorescence after DNA amplification simultaneously process fluorescence excitation and emission for dozens of tubes. Therefore, there is a limit to the size, cost, and assembly of the optical element. In recent years, imaging devices for high-performance, open platforms have benefitted from significant innovations. In this paper, we propose a fluorescence detector for real-time PCR devices using an open platform camera. This system can reduce the cost, and can be miniaturized. To simplify the optical system, four low-cost, compact cameras were used. In addition, the field of view of the entire tube was minimized by dividing it into quadrants. An effective image processing method was used to compensate for the reduction in the signal-to-noise ratio. Using a reference fluorescence material, it was confirmed that the proposed system enables stable fluorescence detection according to the amount of DNA.Seul-Bit-Na KooHyeon-Gyu ChiJong-Dae KimYu-Seop KimJi-Sung ParkChan-Young ParkDeuk-Ju LeeMDPI AGarticlereal-time PCRfluorescence detectionopen platformimage processingChemical technologyTP1-1185ENSensors, Vol 21, Iss 7013, p 7013 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	real-time PCR fluorescence detection open platform image processing Chemical technology TP1-1185
spellingShingle	real-time PCR fluorescence detection open platform image processing Chemical technology TP1-1185 Seul-Bit-Na Koo Hyeon-Gyu Chi Jong-Dae Kim Yu-Seop Kim Ji-Sung Park Chan-Young Park Deuk-Ju Lee Multiple Compact Camera Fluorescence Detector for Real-Time PCR Devices
description	The polymerase chain reaction is an important technique in biological research because it tests for diseases with a small amount of DNA. However, this process is time consuming and can lead to sample contamination. Recently, real-time PCR techniques have emerged which make it possible to monitor the amplification process for each cycle in real time. Existing camera-based systems that measure fluorescence after DNA amplification simultaneously process fluorescence excitation and emission for dozens of tubes. Therefore, there is a limit to the size, cost, and assembly of the optical element. In recent years, imaging devices for high-performance, open platforms have benefitted from significant innovations. In this paper, we propose a fluorescence detector for real-time PCR devices using an open platform camera. This system can reduce the cost, and can be miniaturized. To simplify the optical system, four low-cost, compact cameras were used. In addition, the field of view of the entire tube was minimized by dividing it into quadrants. An effective image processing method was used to compensate for the reduction in the signal-to-noise ratio. Using a reference fluorescence material, it was confirmed that the proposed system enables stable fluorescence detection according to the amount of DNA.
format	article
author	Seul-Bit-Na Koo Hyeon-Gyu Chi Jong-Dae Kim Yu-Seop Kim Ji-Sung Park Chan-Young Park Deuk-Ju Lee
author_facet	Seul-Bit-Na Koo Hyeon-Gyu Chi Jong-Dae Kim Yu-Seop Kim Ji-Sung Park Chan-Young Park Deuk-Ju Lee
author_sort	Seul-Bit-Na Koo
title	Multiple Compact Camera Fluorescence Detector for Real-Time PCR Devices
title_short	Multiple Compact Camera Fluorescence Detector for Real-Time PCR Devices
title_full	Multiple Compact Camera Fluorescence Detector for Real-Time PCR Devices
title_fullStr	Multiple Compact Camera Fluorescence Detector for Real-Time PCR Devices
title_full_unstemmed	Multiple Compact Camera Fluorescence Detector for Real-Time PCR Devices
title_sort	multiple compact camera fluorescence detector for real-time pcr devices
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/3485a57b4dc24d898d7f4df42ddf4ab6
work_keys_str_mv	AT seulbitnakoo multiplecompactcamerafluorescencedetectorforrealtimepcrdevices AT hyeongyuchi multiplecompactcamerafluorescencedetectorforrealtimepcrdevices AT jongdaekim multiplecompactcamerafluorescencedetectorforrealtimepcrdevices AT yuseopkim multiplecompactcamerafluorescencedetectorforrealtimepcrdevices AT jisungpark multiplecompactcamerafluorescencedetectorforrealtimepcrdevices AT chanyoungpark multiplecompactcamerafluorescencedetectorforrealtimepcrdevices AT deukjulee multiplecompactcamerafluorescencedetectorforrealtimepcrdevices
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Multiple Compact Camera Fluorescence Detector for Real-Time PCR Devices

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